Matches in SemOpenAlex for { <https://semopenalex.org/work/W2562425248> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2562425248 abstract "Abstract Abstract 2377 FANCD2 is a key player in FA pathway. It has been shown that FANCD2 can interact with PCNA and with Rad18, the ubiquitin ligase responsible for PCNA mono-ubiquitination. The mono-ubiquitination of PCNA is very important for its function in translesion synthesis. We found that in response to DNA damage agent hyxdroxyurea (HU) the interaction of FANCD2 with Rad18 or Rad51, and the interaction of Rad51 with Rad18 or PCNA was enhanced. FANCD2 is required for increased interaction between Rad51 and Rad18 indicating that FANCD2, Rad51 and Rad18 form a complex in response to HU. Rad18 was required for PCNA mono-ubiquitination in response to HU. FANCD2 deficient cells failed to enhance the interaction between Rad18 and Rad51. Furthermore, PCNA mono-ubiquitination was impaired in FANCD2 deficient cells in response to HU. FANCD2 mono-ubiquitination deficient mutant partially rescued PCNA mono-ubiquitination. The partial mono-ubiquitination of PCNA in response to HU in FANCA deficient mutant confirmed the role of non-ubiquitinated FANCD2 in PCNA mono-ubiquitination. The normal mono-ubiquitination of PCNA in FANCJ deficient mutant confirmed that the effect of FANCD2 in PCNA mon-ubiquitination is not due to FA pathway deficiency. Rad51 was also involved in regulating PCNA mono-ubiquitination in response to HU. Rad51 siRNA knock down cells showed decreased PCNA mono-ubiquitination in response to HU. The role of Rad51 in regulating PCNA mono-ubiquitination did not require BRCA1, indicating that this function is independent of HR. More importantly FANCD2 deficient cells were hypersensitive to HU, whereas FANCD2 mono-ubiquitination deficient mutant cells, FANCD2 corrected cells, FANCA deficient cells and FANCJ deficient cells were not hypersensitive to HU. Our data indicate that FANCD2 plays an important role in PCNA mono-ubiquitination and translesion synthesis partially in a mono-ubiquitination independent manner. Rad51 also plays an important role in PCNA mono-ubiquitination and translesion synthesis in a HR independent fashion. Disclosures: No relevant conflicts of interest to declare." @default.
- W2562425248 created "2017-01-06" @default.
- W2562425248 creator A5022759903 @default.
- W2562425248 creator A5079834643 @default.
- W2562425248 date "2012-11-16" @default.
- W2562425248 modified "2023-09-27" @default.
- W2562425248 title "Regulation of PCNA Mono-Ubiqutination by FANCD2 and Rad51 in Response to Hydroxyurea." @default.
- W2562425248 doi "https://doi.org/10.1182/blood.v120.21.2377.2377" @default.
- W2562425248 hasPublicationYear "2012" @default.
- W2562425248 type Work @default.
- W2562425248 sameAs 2562425248 @default.
- W2562425248 citedByCount "0" @default.
- W2562425248 crossrefType "journal-article" @default.
- W2562425248 hasAuthorship W2562425248A5022759903 @default.
- W2562425248 hasAuthorship W2562425248A5079834643 @default.
- W2562425248 hasConcept C104317684 @default.
- W2562425248 hasConcept C134459356 @default.
- W2562425248 hasConcept C134935766 @default.
- W2562425248 hasConcept C143425029 @default.
- W2562425248 hasConcept C153911025 @default.
- W2562425248 hasConcept C185592680 @default.
- W2562425248 hasConcept C25602115 @default.
- W2562425248 hasConcept C2776653068 @default.
- W2562425248 hasConcept C2778124228 @default.
- W2562425248 hasConcept C2778993383 @default.
- W2562425248 hasConcept C2779012473 @default.
- W2562425248 hasConcept C34688535 @default.
- W2562425248 hasConcept C552990157 @default.
- W2562425248 hasConcept C55493867 @default.
- W2562425248 hasConcept C86803240 @default.
- W2562425248 hasConcept C95444343 @default.
- W2562425248 hasConceptScore W2562425248C104317684 @default.
- W2562425248 hasConceptScore W2562425248C134459356 @default.
- W2562425248 hasConceptScore W2562425248C134935766 @default.
- W2562425248 hasConceptScore W2562425248C143425029 @default.
- W2562425248 hasConceptScore W2562425248C153911025 @default.
- W2562425248 hasConceptScore W2562425248C185592680 @default.
- W2562425248 hasConceptScore W2562425248C25602115 @default.
- W2562425248 hasConceptScore W2562425248C2776653068 @default.
- W2562425248 hasConceptScore W2562425248C2778124228 @default.
- W2562425248 hasConceptScore W2562425248C2778993383 @default.
- W2562425248 hasConceptScore W2562425248C2779012473 @default.
- W2562425248 hasConceptScore W2562425248C34688535 @default.
- W2562425248 hasConceptScore W2562425248C552990157 @default.
- W2562425248 hasConceptScore W2562425248C55493867 @default.
- W2562425248 hasConceptScore W2562425248C86803240 @default.
- W2562425248 hasConceptScore W2562425248C95444343 @default.
- W2562425248 hasLocation W25624252481 @default.
- W2562425248 hasOpenAccess W2562425248 @default.
- W2562425248 hasPrimaryLocation W25624252481 @default.
- W2562425248 hasRelatedWork W1966240299 @default.
- W2562425248 hasRelatedWork W1971580036 @default.
- W2562425248 hasRelatedWork W1989575399 @default.
- W2562425248 hasRelatedWork W2012485321 @default.
- W2562425248 hasRelatedWork W2017329539 @default.
- W2562425248 hasRelatedWork W2017896723 @default.
- W2562425248 hasRelatedWork W2023489614 @default.
- W2562425248 hasRelatedWork W2046962987 @default.
- W2562425248 hasRelatedWork W2088614788 @default.
- W2562425248 hasRelatedWork W2094849013 @default.
- W2562425248 hasRelatedWork W2095687961 @default.
- W2562425248 hasRelatedWork W2105513499 @default.
- W2562425248 hasRelatedWork W2156894557 @default.
- W2562425248 hasRelatedWork W2165721639 @default.
- W2562425248 hasRelatedWork W2489904994 @default.
- W2562425248 hasRelatedWork W2552320475 @default.
- W2562425248 hasRelatedWork W2606601579 @default.
- W2562425248 hasRelatedWork W2980084371 @default.
- W2562425248 hasRelatedWork W3125738453 @default.
- W2562425248 hasRelatedWork W3184890740 @default.
- W2562425248 isParatext "false" @default.
- W2562425248 isRetracted "false" @default.
- W2562425248 magId "2562425248" @default.
- W2562425248 workType "article" @default.