Matches in SemOpenAlex for { <https://semopenalex.org/work/W2562537731> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2562537731 abstract "MicroRNAs (miRNA/miRs) play a crucial role in a variety of biological processes including stem cell differentiation and function. Foetal femur-derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity, indicating excellent potential as candidates for tissue engineering applications. This thesis has identified and characterised subpopulations of skeletal stem cells found within the foetal femur. Cells isolated from the epiphyseal region of the foetal femur expressed higher levels of genes associated with chondrogenesis while cells from the diaphyseal region expressed higher levels of genes associated with osteogenic differentiation. In addition to the difference in osteogenic and chondrogenic gene expression, epiphyseal and diaphyseal cell populations displayed distinct miRs expression profiles. To examine the role of miRNA during skeletogenesis, a robust cell culture model containing differentiating SSCs and an effective transfection protocol was developed. Spermine-pullulan complex, a potential delivery system for miRNA-based gene therapy, was shown to be able to transfect SSCs with miRNA mimics and inhibitors but with lower efficacy compared to liposome base transfection reagent. Through miRNA gene expression profiling and mRNA targets analysis, miR-146a was found to be expressed by diaphyseal cell populations at a significantly enhanced level compared to epiphyseal populations and was predicted to target various components of the TGF-? pathway. Examination of miR-146a function in foetal femur cells confirmed regulation of protein translation of SMAD2 and SMAD3 following transient overexpression in epiphyseal cells. The down-regulation of SMAD2 and SMAD3 following overexpression of miR-146a resulted in an up-regulation of the osteogenesis-related gene RUNX2 and down-regulation of the chondrogenesis-related gene SOX9.In conclusion, this thesis has identified subpopulations of skeletal stem cells with enhanced osteogenic and chondrogenic potential and has explored new miRNA targets involved in skeletogenesis in the attempt to develop novel treatments for patients requiring reparation of the skeletal system." @default.
- W2562537731 created "2017-01-06" @default.
- W2562537731 creator A5050640527 @default.
- W2562537731 date "2015-09-01" @default.
- W2562537731 modified "2023-09-23" @default.
- W2562537731 title "Examination of MicroRNAs in skeletal stem cell differentiation" @default.
- W2562537731 hasPublicationYear "2015" @default.
- W2562537731 type Work @default.
- W2562537731 sameAs 2562537731 @default.
- W2562537731 citedByCount "0" @default.
- W2562537731 crossrefType "dissertation" @default.
- W2562537731 hasAuthorship W2562537731A5050640527 @default.
- W2562537731 hasConcept C104317684 @default.
- W2562537731 hasConcept C145059251 @default.
- W2562537731 hasConcept C148738053 @default.
- W2562537731 hasConcept C150194340 @default.
- W2562537731 hasConcept C2776363554 @default.
- W2562537731 hasConcept C28328180 @default.
- W2562537731 hasConcept C35496256 @default.
- W2562537731 hasConcept C54009773 @default.
- W2562537731 hasConcept C54355233 @default.
- W2562537731 hasConcept C81885089 @default.
- W2562537731 hasConcept C86803240 @default.
- W2562537731 hasConcept C95444343 @default.
- W2562537731 hasConceptScore W2562537731C104317684 @default.
- W2562537731 hasConceptScore W2562537731C145059251 @default.
- W2562537731 hasConceptScore W2562537731C148738053 @default.
- W2562537731 hasConceptScore W2562537731C150194340 @default.
- W2562537731 hasConceptScore W2562537731C2776363554 @default.
- W2562537731 hasConceptScore W2562537731C28328180 @default.
- W2562537731 hasConceptScore W2562537731C35496256 @default.
- W2562537731 hasConceptScore W2562537731C54009773 @default.
- W2562537731 hasConceptScore W2562537731C54355233 @default.
- W2562537731 hasConceptScore W2562537731C81885089 @default.
- W2562537731 hasConceptScore W2562537731C86803240 @default.
- W2562537731 hasConceptScore W2562537731C95444343 @default.
- W2562537731 hasLocation W25625377311 @default.
- W2562537731 hasOpenAccess W2562537731 @default.
- W2562537731 hasPrimaryLocation W25625377311 @default.
- W2562537731 hasRelatedWork W1917037591 @default.
- W2562537731 hasRelatedWork W1939946462 @default.
- W2562537731 hasRelatedWork W1979121282 @default.
- W2562537731 hasRelatedWork W1981052020 @default.
- W2562537731 hasRelatedWork W1988510529 @default.
- W2562537731 hasRelatedWork W2098687127 @default.
- W2562537731 hasRelatedWork W2127686290 @default.
- W2562537731 hasRelatedWork W2232740985 @default.
- W2562537731 hasRelatedWork W2334884500 @default.
- W2562537731 hasRelatedWork W2379280353 @default.
- W2562537731 hasRelatedWork W2592879677 @default.
- W2562537731 hasRelatedWork W2729392910 @default.
- W2562537731 hasRelatedWork W2770552652 @default.
- W2562537731 hasRelatedWork W2982423780 @default.
- W2562537731 hasRelatedWork W2999141169 @default.
- W2562537731 hasRelatedWork W3026201920 @default.
- W2562537731 hasRelatedWork W3047432161 @default.
- W2562537731 hasRelatedWork W3091947008 @default.
- W2562537731 hasRelatedWork W3194243476 @default.
- W2562537731 hasRelatedWork W962052387 @default.
- W2562537731 isParatext "false" @default.
- W2562537731 isRetracted "false" @default.
- W2562537731 magId "2562537731" @default.
- W2562537731 workType "dissertation" @default.