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- W2562702078 abstract "The aim of this work consists of development of a biosensor for urea based on amidase inhibition since urea is a powerful active site inhibitor of this enzyme which catalyses the hydrolysis of amides such as acetamide producing ammonia and the corresponding organic acid. Cell-free extract from Pseudomonas aeruginosa was the source of amidase (acylamide hydrolase EC 3.5.1.4) which was immobilized in polyethersulfone (PES) membrane in the presence glutaraldehyde and ion-selective electrode (ISE) for ammonium ions was used for biosensor development. ANOVA methodology was used for optimization of the biosensor response and hence 30 μL of cell-free extract, 2 μL glutaraldehyde (5%) and 10 μL gelatin 15% (p/v) exhibited the highest response. This biosensor exhibited a linear response in the range of 4.0 – 10.0 M of urea, a detection limit of 2.0 M for urea, a response time of 20 s, a sensitivity of 58.245 %.M -1 of urea concentration and a storage stability of over two months. It was successfully used for quantification of urea in real samples such as wine and recovery experiments were carried out which revealed an average substrate recovery of 93.1%. This biosensor is cheap since cell-free extract of P. aeruginosa can be used as source of amidase activity. The selectivity of this biosensor was investigated towards urea analogues such as hydroxyurea, methylurea and thiourea which revealed that they inhibited amidase activity about 90%, 10% and 0%, respectively compared with urea inhibition." @default.
- W2562702078 created "2017-01-06" @default.
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- W2562702078 date "2010-01-01" @default.
- W2562702078 modified "2023-09-23" @default.
- W2562702078 title "Development of a biosensor for urea assay in wines by ion selective electrode based on amidase inhibition" @default.
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