FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2562947536> ?p ?o ?g. }

• W2562947536 endingPage "3426" @default.
• W2562947536 startingPage "3420" @default.
• W2562947536 abstract "Abstract BACKGROUND d ‐Allulose is a novel and low‐calorie rare monosaccharide that is a C‐3 epimer of d ‐fructose. Because of its excellent physiological properties and commercial potential, d ‐allulose has attracted researchers' interests. Based on the Izumoring strategy, d ‐allulose is converted from d ‐fructose by d ‐psicose 3‐epimerase ( DPEase ), while d ‐fructose is converted from d ‐glucose by d ‐glucose isomerase ( GIase ). In this study, we created a cellular system capable of converting d ‐glucose to d ‐allulose in a one‐step process that co‐expressed the GIase from Acidothermus cellulolyticus and the DPEase from Dorea sp. CAG . RESULTS The co‐expression plasmid pETDuet ‐Dosp‐ DPE /Acce‐ GI was generated and transformed into Escherichia coli BL21 ( DE3 ) cells. The recombinant co‐expression cells exhibited maximum catalytic activity at pH 6.5 and 75 °C. These cells were thermostable at less than 60 °C. The addition of Co 2+ significantly increased the catalytic activity by 10.8‐fold. When the reaction equilibrium was reached, the ratio of d ‐glucose, d ‐fructose and d ‐allulose was approximately 6.5:7:3, respectively. CONCLUSION A recombinant co‐expression strain that catalysed the bioconversion of d ‐allulose from d ‐glucose in a one‐step process was created and characterised. When adding 500 g L −1 d ‐glucose as a substrate, 204.3 g L −1 d ‐fructose and 89.1 g L −1 d ‐allulose were produced. © 2016 Society of Chemical Industry" @default.
• W2562947536 created "2017-01-06" @default.
• W2562947536 creator A5000963442 @default.
• W2562947536 creator A5004392744 @default.
• W2562947536 creator A5019560977 @default.
• W2562947536 creator A5035701638 @default.
• W2562947536 creator A5085774449 @default.
• W2562947536 date "2017-01-17" @default.
• W2562947536 modified "2023-10-17" @default.
• W2562947536 title "Production of <scp>d</scp>‐allulose from <scp>d</scp>‐glucose by <i>Escherichia coli</i> transformant cells co‐expressing <scp>d</scp>‐glucose isomerase and <scp>d</scp>‐psicose 3‐epimerase genes" @default.
• W2562947536 cites W1151175600 @default.
• W2562947536 cites W1586372951 @default.
• W2562947536 cites W1811673022 @default.
• W2562947536 cites W1968782729 @default.
• W2562947536 cites W1970780806 @default.
• W2562947536 cites W1979591684 @default.
• W2562947536 cites W1989703301 @default.
• W2562947536 cites W1993907164 @default.
• W2562947536 cites W1994272480 @default.
• W2562947536 cites W2007828444 @default.
• W2562947536 cites W2009694869 @default.
• W2562947536 cites W2037936279 @default.
• W2562947536 cites W2044183683 @default.
• W2562947536 cites W2046981822 @default.
• W2562947536 cites W2047374426 @default.
• W2562947536 cites W2061275659 @default.
• W2562947536 cites W2089295327 @default.
• W2562947536 cites W2094233192 @default.
• W2562947536 cites W2107405889 @default.
• W2562947536 cites W2114156535 @default.
• W2562947536 cites W2158226129 @default.
• W2562947536 cites W2170182584 @default.
• W2562947536 cites W2325721067 @default.
• W2562947536 cites W2342006594 @default.
• W2562947536 cites W639297711 @default.
• W2562947536 cites W744818514 @default.
• W2562947536 doi "https://doi.org/10.1002/jsfa.8193" @default.
• W2562947536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28009059" @default.
• W2562947536 hasPublicationYear "2017" @default.
• W2562947536 type Work @default.
• W2562947536 sameAs 2562947536 @default.
• W2562947536 citedByCount "20" @default.
• W2562947536 countsByYear W25629475362018 @default.
• W2562947536 countsByYear W25629475362019 @default.
• W2562947536 countsByYear W25629475362020 @default.
• W2562947536 countsByYear W25629475362021 @default.
• W2562947536 countsByYear W25629475362022 @default.
• W2562947536 countsByYear W25629475362023 @default.
• W2562947536 crossrefType "journal-article" @default.
• W2562947536 hasAuthorship W2562947536A5000963442 @default.
• W2562947536 hasAuthorship W2562947536A5004392744 @default.
• W2562947536 hasAuthorship W2562947536A5019560977 @default.
• W2562947536 hasAuthorship W2562947536A5035701638 @default.
• W2562947536 hasAuthorship W2562947536A5085774449 @default.
• W2562947536 hasConcept C100544194 @default.
• W2562947536 hasConcept C104317684 @default.
• W2562947536 hasConcept C118859902 @default.
• W2562947536 hasConcept C181199279 @default.
• W2562947536 hasConcept C185592680 @default.
• W2562947536 hasConcept C18903297 @default.
• W2562947536 hasConcept C203130289 @default.
• W2562947536 hasConcept C2776970464 @default.
• W2562947536 hasConcept C2777289219 @default.
• W2562947536 hasConcept C2780074830 @default.
• W2562947536 hasConcept C2781264208 @default.
• W2562947536 hasConcept C40767141 @default.
• W2562947536 hasConcept C547475151 @default.
• W2562947536 hasConcept C55493867 @default.
• W2562947536 hasConcept C71240020 @default.
• W2562947536 hasConcept C86803240 @default.
• W2562947536 hasConcept C88189869 @default.
• W2562947536 hasConceptScore W2562947536C100544194 @default.
• W2562947536 hasConceptScore W2562947536C104317684 @default.
• W2562947536 hasConceptScore W2562947536C118859902 @default.
• W2562947536 hasConceptScore W2562947536C181199279 @default.
• W2562947536 hasConceptScore W2562947536C185592680 @default.
• W2562947536 hasConceptScore W2562947536C18903297 @default.
• W2562947536 hasConceptScore W2562947536C203130289 @default.
• W2562947536 hasConceptScore W2562947536C2776970464 @default.
• W2562947536 hasConceptScore W2562947536C2777289219 @default.
• W2562947536 hasConceptScore W2562947536C2780074830 @default.
• W2562947536 hasConceptScore W2562947536C2781264208 @default.
• W2562947536 hasConceptScore W2562947536C40767141 @default.
• W2562947536 hasConceptScore W2562947536C547475151 @default.
• W2562947536 hasConceptScore W2562947536C55493867 @default.
• W2562947536 hasConceptScore W2562947536C71240020 @default.
• W2562947536 hasConceptScore W2562947536C86803240 @default.
• W2562947536 hasConceptScore W2562947536C88189869 @default.
• W2562947536 hasFunder F4320321605 @default.
• W2562947536 hasIssue "10" @default.
• W2562947536 hasLocation W25629475361 @default.
• W2562947536 hasLocation W25629475362 @default.
• W2562947536 hasOpenAccess W2562947536 @default.
• W2562947536 hasPrimaryLocation W25629475361 @default.
• W2562947536 hasRelatedWork W10753211 @default.
• W2562947536 hasRelatedWork W1540686090 @default.
• W2562947536 hasRelatedWork W1987475478 @default.
• W2562947536 hasRelatedWork W1996815989 @default.

• next