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• W2563103460 abstract "Abstract Delivery and expression of recombinant genes, a key methodology for many applications in biological research, remains a challenge especially for mature neurons. Here, we report easy, highly efficient and well tolerated transduction of adult peripheral and central neuronal populations of diverse species in culture using VSV-G pseudo-typed, recombinant baculovirus (BacMam). Transduction rates of up to 80% were reliably achieved at high multiplicity of infection without apparent neuro-cytopathic effects. Neurons could be transduced either shortly after plating or after several days in culture. Co-incubation with two different baculoviruses attained near complete co-localization of fluorescent protein expression, indicating multigene delivery. Finally, evidence for functional protein expression is provided by means of cre-mediated genetic recombination and neurite outgrowth assays. Recombinant protein was already detected within hours after transduction, thereby enabling functional readouts even in relatively short-lived neuronal cultures. Altogether, these results substantiate the usefulness of baculovirus-mediated transduction of mature neurons for future research in neuroscience." @default.
• W2563103460 created "2017-01-06" @default.
• W2563103460 creator A5000518705 @default.
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• W2563103460 date "2016-12-13" @default.
• W2563103460 modified "2023-10-18" @default.
• W2563103460 title "Highly efficient transduction of primary adult CNS and PNS neurons" @default.
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• W2563103460 doi "https://doi.org/10.1038/srep38928" @default.
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