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- W2563194245 abstract "目的探讨RNA干扰p38基因对人肾癌细胞株786-O增殖、侵袭、细胞周期和细胞对舒尼替尼敏感性的影响。 方法构建针对p38的siRNA531和siRNA659两条siRNA，分别将其转染至肾癌786-O细胞株，即为siRNA531组和siRNA659组，同时设置转染无义siRNA的阴性对照组和仅加转染试剂的空白对照组。应用RT-PCR技术检测786-O细胞p38 mRNA的表达，蛋白质印迹法检测p38蛋白的表达。CCK-8法检测细胞的增殖情况和对舒尼替尼的敏感性，流式细胞术检测细胞的周期改变情况，Transwell实验检测细胞的侵袭能力。 结果RT-PCR及蛋白质印迹法检测发现siRNA转染后786-O细胞p38 mRNA及蛋白的表达均降低。与空白对照组和阴性对照组相比，siRNA531组和siRNA659组786-O细胞在转染后3~5 d时的增殖率均降低（P < 0.05，P < 0.01），细胞对舒尼替尼的敏感性增加，两组对舒尼替尼的IC50值均低于阴性对照组[（3.2±0.3）、（1.4±0.1）μmol/mL vs（5.4±0.2）μmol/mL，P < 0.05]。siRNA531组、siRNA659组G1期细胞数量明显多于对照组，且两组786-O细胞出现G0/G1阻滞。转染24 h后，两组的穿膜细胞数分别为56.43±6.02、34.00±8.12，与阴性对照组（76.27±5.08）相比，两组细胞的侵袭能力均下降（P < 0. 01）。 结论通过转染p38特异性siRNA可以成功沉默肾癌细胞株786-O的p38基因的表达，抑制肾癌细胞株786-O的增殖、侵袭能力，增加其对舒尼替尼的敏感性，为后续研究肾癌治疗及靶向耐药奠定基础。" @default.
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- W2563194245 date "2016-07-25" @default.
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- W2563194245 title "RNA干扰 p 38基因对肾癌786-O细胞生物学特性的影响及对舒尼替尼的增敏作用" @default.
- W2563194245 doi "https://doi.org/10.16781/j.0258-879x.2016.07.0799" @default.
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