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- W2563311312 abstract "Immunochromatography and enzyme-linked immunosorbent assay (ELISA) represent selective and sensitive procedures based on solid-phases for separation/detection and quantification of anthropogenic pollutants in the aquatic environment. In contrast with batch-wise procedures, such as microplate-based platforms, automated methods reduce manual handling of reagents, thus increasing overall precision and decreasing time-to-result. Microparticles have been shown to be an adequate support for carrying out immunoassays in meso and microfluidic systems. They offer a wide range of coupling sites for biomolecules such as antibodies, combined with specialised anti-fouling surfaces to prevent non-specific binding and high compressibility for optimum fluidics.In this work we investigated the protein-coupling behaviour of two commercially available microsphere supports (Tentagel® polystyrene-PEG-COOH and PolyAn® PMMA beads with 3D antifouling surface) using DCC/EDC and NHS/S-NHS activation chemistry. The study of coupling conditions (pH, proportion of reagents and type of buffering system) was addressed. The success of the biomodification of the supports was demonstrated by using self-prepared fluorophore-protein conjugates (Fig. 1). Laser-scanning microscopy and flow cytometry were applied for further characterization of the functionalized particles. The applicability of the developed particles will be demonstrated through the design of suspension multiplex assays for the detection, quantification and preconcentration of bioactive substances such as caffeine and carbamazepine, using Lab-on-valve (LOV) platforms." @default.
- W2563311312 created "2017-01-06" @default.
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- W2563311312 date "2016-01-01" @default.
- W2563311312 modified "2023-09-26" @default.
- W2563311312 title "Microsphere biofunctionalization for meso/microfluidic-based automated immunoassays" @default.
- W2563311312 hasPublicationYear "2016" @default.
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