FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2563323280> ?p ?o ?g. }

• W2563323280 endingPage "484" @default.
• W2563323280 startingPage "466" @default.
• W2563323280 abstract "The vitamin B 6 salvage pathway, involving pyridoxine 5′‐phosphate oxidase ( PNPO x) and pyridoxal kinase ( PLK ), recycles B 6 vitamers from nutrients and protein turnover to produce pyridoxal 5′‐phosphate ( PLP ), the catalytically active form of the vitamin. Regulation of this pathway, widespread in living organisms including humans and many bacteria, is very important to vitamin B 6 homeostasis but poorly understood. Although some information is available on the enzymatic regulation of PNPO x and PLK , little is known on their regulation at the transcriptional level. In the present work, we identified a new MocR‐like regulator, PtsJ from Salmonella typhimurium , which controls the expression of the pdxK gene encoding one of the two PLK s expressed in this organism ( PLK 1). Analysis of pdxK expression in a ptsJ knockout strain demonstrated that PtsJ acts as a transcriptional repressor. This is the first case of a MocR‐like regulator acting as repressor of its target gene. Expression and purification of PtsJ allowed a detailed characterisation of its effector and DNA ‐binding properties. PLP is the only B 6 vitamer acting as effector molecule for PtsJ. A DNA ‐binding region composed of four repeated nucleotide sequences is responsible for binding of PtsJ to its target promoter. Analysis of binding stoichiometry revealed that protein subunits/ DNA molar ratio varies from 4 : 1 to 2 : 1, depending on the presence or absence of PLP . Structural characteristics of DNA transcriptional factor‐binding sites suggest that PtsJ binds DNA according to a different model with respect to other characterised members of the MocR subgroup." @default.
• W2563323280 created "2017-01-06" @default.
• W2563323280 creator A5015890126 @default.
• W2563323280 creator A5068344981 @default.
• W2563323280 creator A5075305488 @default.
• W2563323280 creator A5078917080 @default.
• W2563323280 creator A5079664131 @default.
• W2563323280 creator A5086546981 @default.
• W2563323280 date "2017-01-09" @default.
• W2563323280 modified "2023-10-16" @default.
• W2563323280 title "<i>Salmonella typhimurium</i>PtsJ is a novel MocR‐like transcriptional repressor involved in regulating the vitamin B₆salvage pathway" @default.
• W2563323280 cites W112985791 @default.
• W2563323280 cites W1489523425 @default.
• W2563323280 cites W1499158185 @default.
• W2563323280 cites W1504513968 @default.
• W2563323280 cites W1549195789 @default.
• W2563323280 cites W1579756873 @default.
• W2563323280 cites W1800383487 @default.
• W2563323280 cites W1951096158 @default.
• W2563323280 cites W1963544263 @default.
• W2563323280 cites W1974089947 @default.
• W2563323280 cites W1976576122 @default.
• W2563323280 cites W1995661119 @default.
• W2563323280 cites W1996741413 @default.
• W2563323280 cites W1999728411 @default.
• W2563323280 cites W2004187867 @default.
• W2563323280 cites W2004408082 @default.
• W2563323280 cites W2017545024 @default.
• W2563323280 cites W2018933730 @default.
• W2563323280 cites W2019284852 @default.
• W2563323280 cites W2028584028 @default.
• W2563323280 cites W2051897400 @default.
• W2563323280 cites W2055016234 @default.
• W2563323280 cites W2060350133 @default.
• W2563323280 cites W2065283382 @default.
• W2563323280 cites W2071679309 @default.
• W2563323280 cites W2088861401 @default.
• W2563323280 cites W2088879573 @default.
• W2563323280 cites W2089528382 @default.
• W2563323280 cites W2107522712 @default.
• W2563323280 cites W2107611103 @default.
• W2563323280 cites W2114605822 @default.
• W2563323280 cites W2115958650 @default.
• W2563323280 cites W2116137883 @default.
• W2563323280 cites W2120772351 @default.
• W2563323280 cites W2121151853 @default.
• W2563323280 cites W2122462087 @default.
• W2563323280 cites W2122581494 @default.
• W2563323280 cites W2127322768 @default.
• W2563323280 cites W2129043348 @default.
• W2563323280 cites W2129890743 @default.
• W2563323280 cites W2131837243 @default.
• W2563323280 cites W2139927751 @default.
• W2563323280 cites W2142284828 @default.
• W2563323280 cites W2145233296 @default.
• W2563323280 cites W2146660498 @default.
• W2563323280 cites W2151956762 @default.
• W2563323280 cites W2157009395 @default.
• W2563323280 cites W2158649512 @default.
• W2563323280 cites W2161425107 @default.
• W2563323280 cites W2164250060 @default.
• W2563323280 cites W2168786813 @default.
• W2563323280 cites W2168909138 @default.
• W2563323280 cites W2168974130 @default.
• W2563323280 cites W2171740941 @default.
• W2563323280 cites W2227516629 @default.
• W2563323280 cites W2328430051 @default.
• W2563323280 cites W2468285161 @default.
• W2563323280 cites W2486133097 @default.
• W2563323280 cites W2508155194 @default.
• W2563323280 cites W2520210297 @default.
• W2563323280 cites W31529086 @default.
• W2563323280 cites W36629840 @default.
• W2563323280 cites W395106337 @default.
• W2563323280 cites W4214672937 @default.
• W2563323280 cites W4247554111 @default.
• W2563323280 cites W4247927165 @default.
• W2563323280 doi "https://doi.org/10.1111/febs.13994" @default.
• W2563323280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27987384" @default.
• W2563323280 hasPublicationYear "2017" @default.
• W2563323280 type Work @default.
• W2563323280 sameAs 2563323280 @default.
• W2563323280 citedByCount "14" @default.
• W2563323280 countsByYear W25633232802017 @default.
• W2563323280 countsByYear W25633232802018 @default.
• W2563323280 countsByYear W25633232802019 @default.
• W2563323280 countsByYear W25633232802020 @default.
• W2563323280 countsByYear W25633232802021 @default.
• W2563323280 countsByYear W25633232802022 @default.
• W2563323280 countsByYear W25633232802023 @default.
• W2563323280 crossrefType "journal-article" @default.
• W2563323280 hasAuthorship W2563323280A5015890126 @default.
• W2563323280 hasAuthorship W2563323280A5068344981 @default.
• W2563323280 hasAuthorship W2563323280A5075305488 @default.
• W2563323280 hasAuthorship W2563323280A5078917080 @default.
• W2563323280 hasAuthorship W2563323280A5079664131 @default.
• W2563323280 hasAuthorship W2563323280A5086546981 @default.
• W2563323280 hasBestOaLocation W25633232801 @default.

• next