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- W2563330552 abstract "The use of next-generation sequencing (NGS) techniques to analyze the genomes of cancer cells has identified numerous genomic alterations, including single-base substitutions, small insertions and deletions, amplification, recombination, and epigenetic modifications. NGS contributes to the clinical management of patients as well as new discoveries that identify the mechanisms of tumorigenesis. Moreover, analysis of gene panels targeting actionable mutations enhances efforts to optimize the selection of chemotherapeutic regimens. However, whole genome sequencing takes several days and costs at least $10,000, depending on sequence coverage. Therefore, laboratories with relatively limited resources must employ a more economical approach. For this purpose, we conducted an integrated nucleotide sequence analysis of a panel of 409-cancer related genes (409-CRG) combined with whole exome sequencing (WES). Analysis of the 409-CRG panel detected low-frequency variants with high sensitivity, and WES identified moderate and high frequency somatic variants as well as germline variants." @default.
- W2563330552 created "2017-01-06" @default.
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- W2563330552 date "2016-01-01" @default.
- W2563330552 modified "2023-10-05" @default.
- W2563330552 title "<b>Integrated next-generation sequencing analysis of whole exome and 409 cancer-related </b><b>genes </b>" @default.
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- W2563330552 doi "https://doi.org/10.2220/biomedres.37.367" @default.
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