Matches in SemOpenAlex for { <https://semopenalex.org/work/W2563616287> ?p ?o ?g. }
- W2563616287 abstract "N-methyl-d-aspartate (NMDA) is expressed in sensory neurons and plays important roles in peripheral pain mechanisms. The aim of this study was to examine the effects and molecular mechanisms of NMDA on C2C12 myoblast proliferation and differentiation.Cytotoxicity and differentiation were examined by the MTT assay, reverse transcription-polymerase chain reaction, and immunofluorescence.NMDA had no cytotoxicity (10-500 μM) and inhibited myoblastic differentiation of C2C12 cells, as assessed by F-actin immunofluorescence and levels of mRNAs encoding myogenic markers such as myogenin and myosin heavy-chain 2. It inhibited phosphorylation of mammalian target of rapamycin (mTOR) by inactivating mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38. It induced reactive oxygen species production. Furthermore, NMDA-suppressed expression of F-actin was reversed by adding the antioxidant N-acetylcysteine.Collectively, these results indicate that NMDA impairs myogenesis or myogenic differentiation in C2C12 cells through the mTOR/MAPK signaling pathways and may lead to skeletal muscle degeneration. Muscle Nerve 56: 510-518, 2017." @default.
- W2563616287 created "2017-01-06" @default.
- W2563616287 creator A5000243896 @default.
- W2563616287 creator A5009992885 @default.
- W2563616287 creator A5023558955 @default.
- W2563616287 creator A5059946880 @default.
- W2563616287 creator A5071993898 @default.
- W2563616287 creator A5074145073 @default.
- W2563616287 creator A5079783667 @default.
- W2563616287 creator A5083909587 @default.
- W2563616287 date "2017-03-10" @default.
- W2563616287 modified "2023-09-23" @default.
- W2563616287 title "N-methyl-D-aspartate (NMDA) impairs myogenesis in C2C12 cells" @default.
- W2563616287 cites W1579420474 @default.
- W2563616287 cites W1855149220 @default.
- W2563616287 cites W1965393437 @default.
- W2563616287 cites W1972880994 @default.
- W2563616287 cites W1976017029 @default.
- W2563616287 cites W1992120609 @default.
- W2563616287 cites W1997642684 @default.
- W2563616287 cites W1999388027 @default.
- W2563616287 cites W2001889965 @default.
- W2563616287 cites W2005101201 @default.
- W2563616287 cites W2010125977 @default.
- W2563616287 cites W2012785149 @default.
- W2563616287 cites W2013381376 @default.
- W2563616287 cites W2017429680 @default.
- W2563616287 cites W2020527205 @default.
- W2563616287 cites W2036909935 @default.
- W2563616287 cites W2037858774 @default.
- W2563616287 cites W2045093346 @default.
- W2563616287 cites W2049342497 @default.
- W2563616287 cites W2051031919 @default.
- W2563616287 cites W2055162922 @default.
- W2563616287 cites W2059328349 @default.
- W2563616287 cites W2069826620 @default.
- W2563616287 cites W2070262598 @default.
- W2563616287 cites W2074214146 @default.
- W2563616287 cites W2075619117 @default.
- W2563616287 cites W2080542307 @default.
- W2563616287 cites W2081070804 @default.
- W2563616287 cites W2093870387 @default.
- W2563616287 cites W2097693778 @default.
- W2563616287 cites W2103671788 @default.
- W2563616287 cites W2108596837 @default.
- W2563616287 cites W2115129249 @default.
- W2563616287 cites W2118256368 @default.
- W2563616287 cites W2122857747 @default.
- W2563616287 cites W2123774200 @default.
- W2563616287 cites W2135789331 @default.
- W2563616287 cites W2136788290 @default.
- W2563616287 cites W2145265436 @default.
- W2563616287 cites W2159524128 @default.
- W2563616287 cites W2163819745 @default.
- W2563616287 cites W2165015386 @default.
- W2563616287 cites W2171262890 @default.
- W2563616287 cites W2171700358 @default.
- W2563616287 cites W2177035195 @default.
- W2563616287 cites W2209257886 @default.
- W2563616287 cites W2411270039 @default.
- W2563616287 cites W4235314908 @default.
- W2563616287 doi "https://doi.org/10.1002/mus.25511" @default.
- W2563616287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27977864" @default.
- W2563616287 hasPublicationYear "2017" @default.
- W2563616287 type Work @default.
- W2563616287 sameAs 2563616287 @default.
- W2563616287 citedByCount "2" @default.
- W2563616287 countsByYear W25636162872017 @default.
- W2563616287 countsByYear W25636162872020 @default.
- W2563616287 crossrefType "journal-article" @default.
- W2563616287 hasAuthorship W2563616287A5000243896 @default.
- W2563616287 hasAuthorship W2563616287A5009992885 @default.
- W2563616287 hasAuthorship W2563616287A5023558955 @default.
- W2563616287 hasAuthorship W2563616287A5059946880 @default.
- W2563616287 hasAuthorship W2563616287A5071993898 @default.
- W2563616287 hasAuthorship W2563616287A5074145073 @default.
- W2563616287 hasAuthorship W2563616287A5079783667 @default.
- W2563616287 hasAuthorship W2563616287A5083909587 @default.
- W2563616287 hasConcept C153911025 @default.
- W2563616287 hasConcept C170493617 @default.
- W2563616287 hasConcept C184235292 @default.
- W2563616287 hasConcept C185592680 @default.
- W2563616287 hasConcept C207200792 @default.
- W2563616287 hasConcept C2778106681 @default.
- W2563616287 hasConcept C3763915 @default.
- W2563616287 hasConcept C55493867 @default.
- W2563616287 hasConcept C60278653 @default.
- W2563616287 hasConcept C67018056 @default.
- W2563616287 hasConcept C6997183 @default.
- W2563616287 hasConcept C86803240 @default.
- W2563616287 hasConcept C95444343 @default.
- W2563616287 hasConcept C97816823 @default.
- W2563616287 hasConceptScore W2563616287C153911025 @default.
- W2563616287 hasConceptScore W2563616287C170493617 @default.
- W2563616287 hasConceptScore W2563616287C184235292 @default.
- W2563616287 hasConceptScore W2563616287C185592680 @default.
- W2563616287 hasConceptScore W2563616287C207200792 @default.
- W2563616287 hasConceptScore W2563616287C2778106681 @default.
- W2563616287 hasConceptScore W2563616287C3763915 @default.
- W2563616287 hasConceptScore W2563616287C55493867 @default.