FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564011643> ?p ?o ?g. }

• W2564011643 endingPage "128" @default.
• W2564011643 startingPage "121" @default.
• W2564011643 abstract "Single nucleotide polymorphism (SNP) genotyping is an important aspect in understanding genetic variations. Here, we discriminate SNPs using toe-hold mediated displacement reactions. The biological target is an 80 nucleotide long double-stranded–DNA from the mtDNA HV1 region, associated with maternal ancestry. This target has been specially designed with a pendant toehold and a cationic fluorophore, ATTO 647N, as a reporter, produced in a polymerase chain reaction. Rates of reaction for the toehold-polymerase chain reaction products (TPPs) with their corresponding complementary displacing sequences, labelled with a Black Hole Quencher 1, followed the order TPP–Cytosine > TPP–Thymine > TPP–Adenine ≥ TPP–Guanine. Non-complementary rates were the slowest with mismatches involving cytosine. These reactions, operating in a static/or contact mode, gave averaged readouts between SNPs within 15 min (with 80–90% quenching), compared to 25–30 min in previous studies involving fluorescence resonance energy transfer. Addition of an intercalating agent, ethidium bromide, retarded the rate of reaction in which cytosine was involved, presumably through stabilization of the base pairing, which resulted in markedly improved discrimination of cytosine containing SNPs." @default.
• W2564011643 created "2017-01-06" @default.
• W2564011643 creator A5009672574 @default.
• W2564011643 creator A5018568646 @default.
• W2564011643 creator A5071138731 @default.
• W2564011643 date "2017-02-01" @default.
• W2564011643 modified "2023-09-27" @default.
• W2564011643 title "Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator" @default.
• W2564011643 cites W1596194860 @default.
• W2564011643 cites W1973038490 @default.
• W2564011643 cites W1980066896 @default.
• W2564011643 cites W1980301976 @default.
• W2564011643 cites W1991198187 @default.
• W2564011643 cites W1997649027 @default.
• W2564011643 cites W2005366093 @default.
• W2564011643 cites W2005615084 @default.
• W2564011643 cites W2013676609 @default.
• W2564011643 cites W2013815807 @default.
• W2564011643 cites W2022257660 @default.
• W2564011643 cites W2029880387 @default.
• W2564011643 cites W2033172935 @default.
• W2564011643 cites W2042932259 @default.
• W2564011643 cites W2047759589 @default.
• W2564011643 cites W2076051033 @default.
• W2564011643 cites W2077433900 @default.
• W2564011643 cites W2087657020 @default.
• W2564011643 cites W2100404753 @default.
• W2564011643 cites W2105213087 @default.
• W2564011643 cites W2116791963 @default.
• W2564011643 cites W2125181285 @default.
• W2564011643 cites W2125882871 @default.
• W2564011643 cites W2143797817 @default.
• W2564011643 cites W2148550663 @default.
• W2564011643 cites W2157035721 @default.
• W2564011643 cites W2316952090 @default.
• W2564011643 cites W2329380962 @default.
• W2564011643 cites W4242425750 @default.
• W2564011643 doi "https://doi.org/10.1016/j.aca.2016.12.011" @default.
• W2564011643 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28081806" @default.
• W2564011643 hasPublicationYear "2017" @default.
• W2564011643 type Work @default.
• W2564011643 sameAs 2564011643 @default.
• W2564011643 citedByCount "4" @default.
• W2564011643 countsByYear W25640116432017 @default.
• W2564011643 countsByYear W25640116432019 @default.
• W2564011643 countsByYear W25640116432023 @default.
• W2564011643 crossrefType "journal-article" @default.
• W2564011643 hasAuthorship W2564011643A5009672574 @default.
• W2564011643 hasAuthorship W2564011643A5018568646 @default.
• W2564011643 hasAuthorship W2564011643A5071138731 @default.
• W2564011643 hasConcept C104317684 @default.
• W2564011643 hasConcept C12554922 @default.
• W2564011643 hasConcept C129312508 @default.
• W2564011643 hasConcept C135763542 @default.
• W2564011643 hasConcept C153209595 @default.
• W2564011643 hasConcept C185592680 @default.
• W2564011643 hasConcept C2776023528 @default.
• W2564011643 hasConcept C2776858809 @default.
• W2564011643 hasConcept C2776907736 @default.
• W2564011643 hasConcept C2778301229 @default.
• W2564011643 hasConcept C512185932 @default.
• W2564011643 hasConcept C552990157 @default.
• W2564011643 hasConcept C55493867 @default.
• W2564011643 hasConcept C71240020 @default.
• W2564011643 hasConcept C86803240 @default.
• W2564011643 hasConceptScore W2564011643C104317684 @default.
• W2564011643 hasConceptScore W2564011643C12554922 @default.
• W2564011643 hasConceptScore W2564011643C129312508 @default.
• W2564011643 hasConceptScore W2564011643C135763542 @default.
• W2564011643 hasConceptScore W2564011643C153209595 @default.
• W2564011643 hasConceptScore W2564011643C185592680 @default.
• W2564011643 hasConceptScore W2564011643C2776023528 @default.
• W2564011643 hasConceptScore W2564011643C2776858809 @default.
• W2564011643 hasConceptScore W2564011643C2776907736 @default.
• W2564011643 hasConceptScore W2564011643C2778301229 @default.
• W2564011643 hasConceptScore W2564011643C512185932 @default.
• W2564011643 hasConceptScore W2564011643C552990157 @default.
• W2564011643 hasConceptScore W2564011643C55493867 @default.
• W2564011643 hasConceptScore W2564011643C71240020 @default.
• W2564011643 hasConceptScore W2564011643C86803240 @default.
• W2564011643 hasFunder F4320334704 @default.
• W2564011643 hasLocation W25640116431 @default.
• W2564011643 hasLocation W25640116432 @default.
• W2564011643 hasOpenAccess W2564011643 @default.
• W2564011643 hasPrimaryLocation W25640116431 @default.
• W2564011643 hasRelatedWork W1974371829 @default.
• W2564011643 hasRelatedWork W1998997455 @default.
• W2564011643 hasRelatedWork W2009628452 @default.
• W2564011643 hasRelatedWork W2025570948 @default.
• W2564011643 hasRelatedWork W2099637298 @default.
• W2564011643 hasRelatedWork W2335318587 @default.
• W2564011643 hasRelatedWork W2550836624 @default.
• W2564011643 hasRelatedWork W3208480667 @default.
• W2564011643 hasRelatedWork W3209860468 @default.
• W2564011643 hasRelatedWork W4243087761 @default.
• W2564011643 hasVolume "954" @default.
• W2564011643 isParatext "false" @default.
• W2564011643 isRetracted "false" @default.

• next