FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564071352> ?p ?o ?g. }

• W2564071352 endingPage "305" @default.
• W2564071352 startingPage "297" @default.
• W2564071352 abstract "Introduction To investigate the roles of semaphorin 4D (Sema4D)/plexin-B1 signaling on the angiogenic potential and osteo-/odontogenic differentiation of human dental pulp stem cells (DPSCs) and to uncover the corresponding molecular mechanisms. Methods DPSCs were treated with Sema4D (10 μg/mL) for different time durations. Osteo-/odontogenic differentiation was assessed by quantifying alkaline phosphatase activity, mineralized nodule formation, and osteo-/odontogenic gene (ALP, Col1A1, BSP, RUNX2, and DSPP) and protein (Col1A1 and DSPP) expression. Involvement of the Sema4D/plexin-B1 signaling pathway was analyzed by Western blot analysis. Additionally, angiogenic gene and protein expression was assessed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. In vitro endothelial tube formation assay on Matrigel (BD Biosciences, San Jose, CA) was performed to evaluate the angiogenic inductive potential of the Sema4D-treated DPSCs conditioned medium. Results were analyzed using 1-way analysis of variance and the Student t test. Results Sema4D significantly inhibited ALP activity and mineralized nodule formation of DPSCs. Furthermore, Sema4D-treated DPSCs displayed marked down-regulation in the expression of osteo-/odontogenic genes (ALP, Col1A1, BSP, RUNX2, and DSPP) as well as proteins (Col1A1 and DSPP). Elevated levels of plexin-B1 and downstream RhoA protein expression together with phosphorylated plexin-B1 confirmed the involvement of Sema4D/plexin-B1 signaling. Protein expression of ErbB2 was up-regulated, and Met was slightly down-regulated. Furthermore, Sema4D-treated DPSCs exhibited enhanced expression of vascular endothelial growth factor at both the messenger RNA and protein level. Accordingly, the conditioned medium of Sema4D-treated DPSCs promoted the formation of vessel-like structures as shown by the Matrigel assay. Conclusions Sema4D markedly enhances the angiogenic potential but suppresses osteo-/odontogenic differentiation of DPSCs. Sema4D/plexin-B signaling was activated via the RhoA-mediated pathway." @default.
• W2564071352 created "2017-01-06" @default.
• W2564071352 creator A5006772443 @default.
• W2564071352 creator A5006901857 @default.
• W2564071352 creator A5013616504 @default.
• W2564071352 creator A5019795468 @default.
• W2564071352 creator A5021989292 @default.
• W2564071352 creator A5070741329 @default.
• W2564071352 creator A5082238378 @default.
• W2564071352 date "2017-02-01" @default.
• W2564071352 modified "2023-10-01" @default.
• W2564071352 title "Semaphorin 4D Enhances Angiogenic Potential and Suppresses Osteo-/Odontogenic Differentiation of Human Dental Pulp Stem Cells" @default.
• W2564071352 cites W1243134829 @default.
• W2564071352 cites W1963907322 @default.
• W2564071352 cites W1974771129 @default.
• W2564071352 cites W1983256377 @default.
• W2564071352 cites W2023664549 @default.
• W2564071352 cites W2034807185 @default.
• W2564071352 cites W2041700814 @default.
• W2564071352 cites W2043898271 @default.
• W2564071352 cites W2050312736 @default.
• W2564071352 cites W2057507744 @default.
• W2564071352 cites W2059968898 @default.
• W2564071352 cites W2069221741 @default.
• W2564071352 cites W2070417609 @default.
• W2564071352 cites W2071877754 @default.
• W2564071352 cites W2073676627 @default.
• W2564071352 cites W2078278058 @default.
• W2564071352 cites W2083451205 @default.
• W2564071352 cites W2086792707 @default.
• W2564071352 cites W2093498435 @default.
• W2564071352 cites W2104964652 @default.
• W2564071352 cites W2116864112 @default.
• W2564071352 cites W2130516299 @default.
• W2564071352 cites W2134794992 @default.
• W2564071352 cites W2145040826 @default.
• W2564071352 cites W2153095556 @default.
• W2564071352 cites W2157222066 @default.
• W2564071352 cites W2158556013 @default.
• W2564071352 cites W2171537081 @default.
• W2564071352 cites W2172035295 @default.
• W2564071352 cites W2264005066 @default.
• W2564071352 doi "https://doi.org/10.1016/j.joen.2016.10.019" @default.
• W2564071352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28027822" @default.
• W2564071352 hasPublicationYear "2017" @default.
• W2564071352 type Work @default.
• W2564071352 sameAs 2564071352 @default.
• W2564071352 citedByCount "15" @default.
• W2564071352 countsByYear W25640713522018 @default.
• W2564071352 countsByYear W25640713522019 @default.
• W2564071352 countsByYear W25640713522020 @default.
• W2564071352 countsByYear W25640713522021 @default.
• W2564071352 countsByYear W25640713522022 @default.
• W2564071352 countsByYear W25640713522023 @default.
• W2564071352 crossrefType "journal-article" @default.
• W2564071352 hasAuthorship W2564071352A5006772443 @default.
• W2564071352 hasAuthorship W2564071352A5006901857 @default.
• W2564071352 hasAuthorship W2564071352A5013616504 @default.
• W2564071352 hasAuthorship W2564071352A5019795468 @default.
• W2564071352 hasAuthorship W2564071352A5021989292 @default.
• W2564071352 hasAuthorship W2564071352A5070741329 @default.
• W2564071352 hasAuthorship W2564071352A5082238378 @default.
• W2564071352 hasConcept C104317684 @default.
• W2564071352 hasConcept C150194340 @default.
• W2564071352 hasConcept C153911025 @default.
• W2564071352 hasConcept C2776363554 @default.
• W2564071352 hasConcept C2777093181 @default.
• W2564071352 hasConcept C2777400515 @default.
• W2564071352 hasConcept C2780394083 @default.
• W2564071352 hasConcept C28328180 @default.
• W2564071352 hasConcept C502942594 @default.
• W2564071352 hasConcept C54355233 @default.
• W2564071352 hasConcept C62478195 @default.
• W2564071352 hasConcept C86803240 @default.
• W2564071352 hasConcept C95444343 @default.
• W2564071352 hasConceptScore W2564071352C104317684 @default.
• W2564071352 hasConceptScore W2564071352C150194340 @default.
• W2564071352 hasConceptScore W2564071352C153911025 @default.
• W2564071352 hasConceptScore W2564071352C2776363554 @default.
• W2564071352 hasConceptScore W2564071352C2777093181 @default.
• W2564071352 hasConceptScore W2564071352C2777400515 @default.
• W2564071352 hasConceptScore W2564071352C2780394083 @default.
• W2564071352 hasConceptScore W2564071352C28328180 @default.
• W2564071352 hasConceptScore W2564071352C502942594 @default.
• W2564071352 hasConceptScore W2564071352C54355233 @default.
• W2564071352 hasConceptScore W2564071352C62478195 @default.
• W2564071352 hasConceptScore W2564071352C86803240 @default.
• W2564071352 hasConceptScore W2564071352C95444343 @default.
• W2564071352 hasIssue "2" @default.
• W2564071352 hasLocation W25640713521 @default.
• W2564071352 hasLocation W25640713522 @default.
• W2564071352 hasOpenAccess W2564071352 @default.
• W2564071352 hasPrimaryLocation W25640713521 @default.
• W2564071352 hasRelatedWork W2044899507 @default.
• W2564071352 hasRelatedWork W2068872259 @default.
• W2564071352 hasRelatedWork W2084133967 @default.
• W2564071352 hasRelatedWork W2090372890 @default.
• W2564071352 hasRelatedWork W2142985865 @default.

• next