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- W2564155246 abstract "The application of molecular biology to the study of animal growth has provided many important insights regarding the nature of regulatory proteins and their molecular sequence, structure, interactions, tissue expression, and physiologic regulation during growth. In many cases, the isolation of a growth-related gene precedes any knowledge of protein sequence or structure. In fact, protein sequences are now commonly determined by isolating (cloning) and sequencing the corresponding nuclear gene or messenger RNA(s) (complementary DNA). A variety of strategies and techniques are described for cloning and sequencing growth-related genes of interest These include use of antibodies, synthetic oligodeoxyribonucleotides, binding ligands, and restriction-fragment length polymorphisms for purposes of screening bacteriophage and plasmid clone libraries containing animal DNA molecules. Recombinant DNA technology is continually evolving, as evidenced by the impact of novel polymerase chain reaction methods for rapidly cloning and sequencing DNA. Elucidation of the molecular biology of somatic and cellular growth and differentiation is of fundamental importance. In addition, the continued isolation of growth genes from human and livestock genomes will provide the foundation for the application of biotechnology to enhance animal growth, development, and survival." @default.
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- W2564155246 date "1991-01-01" @default.
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- W2564155246 title "Strategies for identifying, isolating, and sequencing genes of importance in growth biology" @default.
- W2564155246 doi "https://doi.org/10.2527/1991.69suppl_324x" @default.
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