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• W2564183407 abstract "We presented an antibody-free electrochemical technique for the determination of human chorionic gonadotropin (hCG) using silver nanoparticles (AgNPs) as the redox reporters and a hCG-specific binding peptide as the receptor. Peptide-induced AgNPs assembly was achieved on the electrode surface that was modified with the same sequence of peptide (recognition element) used in the AgNPs aggregation. As a result, a well-defined linear-sweep voltammetry (LSV) peak was observed. The attachment of hCG on the electrode surface made the peptide probe lose its ability to trigger the in situ formation of the AgNPs-based network architecture on the electrode surface, thus leading to a much attenuated LSV current. The current decreased with the increase of hCG concentration ranging from 1 mIU/mL to 0.2 IU/mL. Consequently, a detection limit of 0.4 mIU/mL was achieved. To demonstrate the amenability of our method, the contents of hCG in human serum/urine samples were determined. The results were consistent with those obtained by the enzyme linked immunosorbent assay (ELISA). Based on the well-defined and amplified electrochemical signal of the AgNPs-based network architecture, our work would also be valuable for the design of novel electrochemical sensors by marrying specific receptors." @default.
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• W2564183407 date "2017-05-01" @default.
• W2564183407 modified "2023-10-13" @default.
• W2564183407 title "Peptide aptamer-based biosensor for the detection of human chorionic gonadotropin by converting silver nanoparticles-based colorimetric assay into sensitive electrochemical analysis" @default.
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• W2564183407 doi "https://doi.org/10.1016/j.snb.2016.12.066" @default.
• W2564183407 hasPublicationYear "2017" @default.
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