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- W2564306597 abstract "Abstract Aflatoxins are type of mycotoxins mainly produced by Aspergillus flavus and a common contaminant of food and grain, posing a serious economic and health problem worldwide. In order to find efficient bacteria to remove or detoxify these mycotoxins, a bacterial strain capable of degrading aflatoxin B1 (AFB1) was isolated from soil samples using a culture medium containing coumarin as the sole carbon source. Based on 16S rRNA gene sequence analysis, this isolate was identified as Bacillus subtilis JSW-1; its further characterization showed that it could inhibit the growth of A. flavus with an inhibition ratio of 58.3% and could degrade AFB1 by 67.2% after incubation at 30 °C for 72 h. The aflatoxin B1-degrading activity of isolate JSW-1 was predominantly attributed to the cell-free supernatant and this activity was found to be heat stable but sensitive to proteinase K treatment, indicating that the extracellular proteins or enzymes are responsible for the AFB1 degradation. In addition, no degradation products of AFB1 could be detected by liquid chromatography-mass spectrometry (LC-MS) analysis, indicating that the parent AFB1 might be biotransformed to compounds with chemical properties different from that of AFB1." @default.
- W2564306597 created "2017-01-06" @default.
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- W2564306597 date "2017-05-01" @default.
- W2564306597 modified "2023-10-18" @default.
- W2564306597 title "Isolation and characterization of a Bacillus subtilis strain with aflatoxin B 1 biodegradation capability" @default.
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- W2564306597 doi "https://doi.org/10.1016/j.foodcont.2016.12.036" @default.
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