FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564454911> ?p ?o ?g. }

• W2564454911 endingPage "e0168572" @default.
• W2564454911 startingPage "e0168572" @default.
• W2564454911 abstract "Background Obstructive sleep apnea (OSA) is associated with the progression of non-alcoholic fatty liver disease (NAFLD) to steatohepatitis and fibrosis. This progression correlates with the severity of OSA-associated hypoxia. In mice with diet induced obesity, hepatic steatosis leads to liver tissue hypoxia, which worsens with exposure to intermittent hypoxia. Emerging data has implicated hepatocyte cell signaling as an important factor in hepatic fibrogenesis. We hypothesized that hepatocyte specific knockout of the oxygen sensing α subunit of hypoxia inducible factor-1 (HIF-1), a master regulator of the global response to hypoxia, may be protective against the development of liver fibrosis.Methods Wild-type mice and mice with hepatocyte-specific HIF-1α knockout (Hif1a-/-hep) were fed a high trans-fat diet for six months, as a model of NAFLD. Hepatic fibrosis was evaluated by Sirius red stain and hydroxyproline assay. Liver enzymes, fasting insulin, and hepatic triglyceride content were also assessed. Hepatocytes were isolated from Hif1a-/-hep mice and wild-type controls and were exposed to sustained hypoxia (1% O2) or normoxia (16% O2) for 24 hours. The culture media was used to reconstitute type I collagen and the resulting matrices were examined for collagen cross-linking.Results Wild-type mice on a high trans-fat diet had 80% more hepatic collagen than Hif1a-/-hep mice (2.21 μg collagen/mg liver tissue, versus 1.23 μg collagen/mg liver tissue, p = 0.03), which was confirmed by Sirius red staining. Body weight, liver weight, mean hepatic triglyceride content, and fasting insulin were similar between groups. Culture media from wild-type mouse hepatocytes exposed to hypoxia allowed for avid collagen cross-linking, but very little cross-linking was seen when hepatocytes were exposed to normoxia, or when hepatocytes from Hif1a-/-hep mice were used in hypoxia or normoxia.Conclusions Hepatocyte HIF-1 mediates an increase in liver fibrosis in a mouse model of NAFLD, perhaps due to liver tissue hypoxia in hepatic steatosis. HIF-1 is necessary for collagen cross-linking in an in vitro model of fibrosis." @default.
• W2564454911 created "2017-01-06" @default.
• W2564454911 creator A5024240048 @default.
• W2564454911 creator A5043417465 @default.
• W2564454911 creator A5056537119 @default.
• W2564454911 creator A5070103781 @default.
• W2564454911 creator A5071172750 @default.
• W2564454911 creator A5076527848 @default.
• W2564454911 date "2016-12-28" @default.
• W2564454911 modified "2023-10-12" @default.
• W2564454911 title "Hepatocyte Hypoxia Inducible Factor-1 Mediates the Development of Liver Fibrosis in a Mouse Model of Nonalcoholic Fatty Liver Disease" @default.
• W2564454911 cites W1488575885 @default.
• W2564454911 cites W1964572117 @default.
• W2564454911 cites W1969660985 @default.
• W2564454911 cites W1975812656 @default.
• W2564454911 cites W1976439025 @default.
• W2564454911 cites W1982513877 @default.
• W2564454911 cites W1985396726 @default.
• W2564454911 cites W1985464718 @default.
• W2564454911 cites W199046047 @default.
• W2564454911 cites W1995856733 @default.
• W2564454911 cites W1999346088 @default.
• W2564454911 cites W2002355437 @default.
• W2564454911 cites W2014718725 @default.
• W2564454911 cites W2020505247 @default.
• W2564454911 cites W2020960905 @default.
• W2564454911 cites W2026677462 @default.
• W2564454911 cites W2026729272 @default.
• W2564454911 cites W2027358194 @default.
• W2564454911 cites W2028841855 @default.
• W2564454911 cites W2033363278 @default.
• W2564454911 cites W2041745240 @default.
• W2564454911 cites W2042592767 @default.
• W2564454911 cites W2048485019 @default.
• W2564454911 cites W2050830271 @default.
• W2564454911 cites W2065239370 @default.
• W2564454911 cites W2075559743 @default.
• W2564454911 cites W2081685920 @default.
• W2564454911 cites W2083748040 @default.
• W2564454911 cites W2095793094 @default.
• W2564454911 cites W2095894499 @default.
• W2564454911 cites W2097662203 @default.
• W2564454911 cites W2098555833 @default.
• W2564454911 cites W2100044667 @default.
• W2564454911 cites W2142310260 @default.
• W2564454911 cites W2144625075 @default.
• W2564454911 cites W2152864160 @default.
• W2564454911 cites W2159583302 @default.
• W2564454911 cites W2163200614 @default.
• W2564454911 cites W2164556013 @default.
• W2564454911 cites W2167904671 @default.
• W2564454911 cites W2333343699 @default.
• W2564454911 cites W4302804107 @default.
• W2564454911 cites W654668309 @default.
• W2564454911 doi "https://doi.org/10.1371/journal.pone.0168572" @default.
• W2564454911 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5193414" @default.
• W2564454911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28030556" @default.
• W2564454911 hasPublicationYear "2016" @default.
• W2564454911 type Work @default.
• W2564454911 sameAs 2564454911 @default.
• W2564454911 citedByCount "77" @default.
• W2564454911 countsByYear W25644549112018 @default.
• W2564454911 countsByYear W25644549112019 @default.
• W2564454911 countsByYear W25644549112020 @default.
• W2564454911 countsByYear W25644549112021 @default.
• W2564454911 countsByYear W25644549112022 @default.
• W2564454911 countsByYear W25644549112023 @default.
• W2564454911 crossrefType "journal-article" @default.
• W2564454911 hasAuthorship W2564454911A5024240048 @default.
• W2564454911 hasAuthorship W2564454911A5043417465 @default.
• W2564454911 hasAuthorship W2564454911A5056537119 @default.
• W2564454911 hasAuthorship W2564454911A5070103781 @default.
• W2564454911 hasAuthorship W2564454911A5071172750 @default.
• W2564454911 hasAuthorship W2564454911A5076527848 @default.
• W2564454911 hasBestOaLocation W25644549111 @default.
• W2564454911 hasConcept C104317684 @default.
• W2564454911 hasConcept C126322002 @default.
• W2564454911 hasConcept C142724271 @default.
• W2564454911 hasConcept C160450060 @default.
• W2564454911 hasConcept C170493617 @default.
• W2564454911 hasConcept C178790620 @default.
• W2564454911 hasConcept C185592680 @default.
• W2564454911 hasConcept C202751555 @default.
• W2564454911 hasConcept C2776200302 @default.
• W2564454911 hasConcept C2776954865 @default.
• W2564454911 hasConcept C2776996007 @default.
• W2564454911 hasConcept C2778772119 @default.
• W2564454911 hasConcept C2779134260 @default.
• W2564454911 hasConcept C2780559512 @default.
• W2564454911 hasConcept C540031477 @default.
• W2564454911 hasConcept C55493867 @default.
• W2564454911 hasConcept C63932345 @default.
• W2564454911 hasConcept C71924100 @default.
• W2564454911 hasConcept C72699923 @default.
• W2564454911 hasConcept C7836513 @default.
• W2564454911 hasConcept C86339819 @default.
• W2564454911 hasConcept C86803240 @default.
• W2564454911 hasConceptScore W2564454911C104317684 @default.

• next