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- W2564517901 abstract "Autographa californica multinucleopolyhedrovirus (AcMNPV) is the best-characterized baculovirus and the platform for well established recombinant protein and vaccine production technologies. In addition to countless examples of recombinant protein production, two commercially available vaccines (Cervarix, and Provenge) are produced via AcMNPV based expression technologies. More recently, Baculovirus based vectors have also garnered attention as gene delivery vectors, including for use in human gene therapy, because of several key characteristics. Baculoviruses can transduce cells of human origin, albeit at MOIs of 100-200, can accommodate large gene insertions (>38 kb), allowing for the inclusion of multiple genes, large promoters, and regulatory elements, are non-replicative in mammalian cells, do not integrate into mammalian chromosomes, and humans lack pre-existing immunity to baculoviruses. In vivo, Baculoviruses have been used to successfully transduce a wide variety of organs from mammalian species, such as mice, rats and rabbit. Despite these reports there is little information on the overall tissue distribution of in vivo delivered baculovirus. Here we test the transduction efficiency of wildtype and pseudotyped baculovirus vectors in various cell lines, and define the biodistribution of these vectors in C57BL/6 mice via intravenous, intrahepatic, and intranasal installation. In vitro, wildtype virus demonstrated good transduction of HEK293 cells and moderate to low transduction of cells originating from the lung and liver. Amongst the pseudotypes tested, the greatest transduction achieved across all cell types (in vitro) was a recombinant displaying a cell-penetrating-peptide-GP64 fusion protein (CPP-GP64), which demonstrated high levels of transduction across all cell lines tested. In vivo, overall transduction by wildtype virus was restricted to the kidneys and liver, and only at moderate to low levels. Similar to the in vitro results, inclusion of a CPP-GP64 greatly enhanced the transduction levels as well as expanded the tissue distribution of in vivo delivered vector." @default.
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- W2564517901 date "2016-05-01" @default.
- W2564517901 modified "2023-10-18" @default.
- W2564517901 title "108. Pseudotyping Baculovirus Based Vectors for Enhanced In Vitro and In Vivo Delivery" @default.
- W2564517901 doi "https://doi.org/10.1016/s1525-0016(16)32917-3" @default.
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