FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564652743> ?p ?o ?g. }

• W2564652743 endingPage "6" @default.
• W2564652743 startingPage "6" @default.
• W2564652743 abstract "In spite of the enormous biological and clinical significance of penicillin-binding protein (PBP)/β-lactamase (βL), few of their many homologs (PBP)/βLs homologs) have been studied crystallographically, and have known functions. Herein, X-ray crystallographic study of a PBP/βL homolog (Rp46) from Ruegeria pomeroyi is described. Multiple sequence alignments indicate that Rp46 has a conserved serine residue within the S70-X-X-K73 motif (Motif I), acting as the catalytic nucleophile. Moreover, an invariant tyrosine residue (Tyr185) and a Trp365-X-Gly motif (Motif III) were also identified. The recombinant Rp46 protein was expressed in Escherichia coli and purified to homogeneity judging from the SDS-PAGE analysis. Rp46 was crystallized using a solution consisting of 20% (w/v) PEG 3000, 0.1 M Tris-HCl, pH 7.0, 0.2 M calcium acetate, and the X-ray diffraction data were collected to a resolution of 1.90 Å with an Rmerge of 7.4%. The crystals of Rp46 belong to the space group I422, with unit cell parameters a = b = 141.26 Å, and c = 119.75. The structure determination and biochemical characterization are in progress. (Synopsis: A penicillin-binding protein/β-lactamase homolog (Rp46) from Ruegeria pomeroyi was identified and crystallized in the space group I4, and the diffraction data were collected to a resolution of 1.90 Å.)" @default.
• W2564652743 created "2017-01-06" @default.
• W2564652743 creator A5004589858 @default.
• W2564652743 creator A5005904390 @default.
• W2564652743 creator A5006021360 @default.
• W2564652743 creator A5063595248 @default.
• W2564652743 creator A5064522116 @default.
• W2564652743 date "2016-12-29" @default.
• W2564652743 modified "2023-09-29" @default.
• W2564652743 title "Identification and Crystallization of Penicillin-Binding Protein/β-Lactamase Homolog (Rp46) from Ruegeria Pomeroyi" @default.
• W2564652743 cites W1253615 @default.
• W2564652743 cites W1539796472 @default.
• W2564652743 cites W1610239570 @default.
• W2564652743 cites W1967591830 @default.
• W2564652743 cites W1967937567 @default.
• W2564652743 cites W1972854297 @default.
• W2564652743 cites W1985563130 @default.
• W2564652743 cites W1995808589 @default.
• W2564652743 cites W2004589024 @default.
• W2564652743 cites W2008632423 @default.
• W2564652743 cites W2010424100 @default.
• W2564652743 cites W2011374678 @default.
• W2564652743 cites W2014694459 @default.
• W2564652743 cites W2014927296 @default.
• W2564652743 cites W2015123447 @default.
• W2564652743 cites W2017772288 @default.
• W2564652743 cites W2018112187 @default.
• W2564652743 cites W2035800315 @default.
• W2564652743 cites W2040662359 @default.
• W2564652743 cites W2042693531 @default.
• W2564652743 cites W2042998381 @default.
• W2564652743 cites W2047905338 @default.
• W2564652743 cites W2052314588 @default.
• W2564652743 cites W2058638812 @default.
• W2564652743 cites W2059313716 @default.
• W2564652743 cites W2081293932 @default.
• W2564652743 cites W2084292396 @default.
• W2564652743 cites W2095220511 @default.
• W2564652743 cites W2105999876 @default.
• W2564652743 cites W2111804766 @default.
• W2564652743 cites W2127322768 @default.
• W2564652743 cites W2148903381 @default.
• W2564652743 cites W2155250313 @default.
• W2564652743 cites W2162786209 @default.
• W2564652743 cites W2180229411 @default.
• W2564652743 cites W2402945284 @default.
• W2564652743 cites W2557567907 @default.
• W2564652743 doi "https://doi.org/10.3390/cryst7010006" @default.
• W2564652743 hasPublicationYear "2016" @default.
• W2564652743 type Work @default.
• W2564652743 sameAs 2564652743 @default.
• W2564652743 citedByCount "3" @default.
• W2564652743 countsByYear W25646527432018 @default.
• W2564652743 countsByYear W25646527432019 @default.
• W2564652743 crossrefType "journal-article" @default.
• W2564652743 hasAuthorship W2564652743A5004589858 @default.
• W2564652743 hasAuthorship W2564652743A5005904390 @default.
• W2564652743 hasAuthorship W2564652743A5006021360 @default.
• W2564652743 hasAuthorship W2564652743A5063595248 @default.
• W2564652743 hasAuthorship W2564652743A5064522116 @default.
• W2564652743 hasBestOaLocation W25646527431 @default.
• W2564652743 hasConcept C104317684 @default.
• W2564652743 hasConcept C153911025 @default.
• W2564652743 hasConcept C174802600 @default.
• W2564652743 hasConcept C185592680 @default.
• W2564652743 hasConcept C2776653525 @default.
• W2564652743 hasConcept C501593827 @default.
• W2564652743 hasConcept C547475151 @default.
• W2564652743 hasConcept C55493867 @default.
• W2564652743 hasConcept C71240020 @default.
• W2564652743 hasConcept C8010536 @default.
• W2564652743 hasConcept C86803240 @default.
• W2564652743 hasConceptScore W2564652743C104317684 @default.
• W2564652743 hasConceptScore W2564652743C153911025 @default.
• W2564652743 hasConceptScore W2564652743C174802600 @default.
• W2564652743 hasConceptScore W2564652743C185592680 @default.
• W2564652743 hasConceptScore W2564652743C2776653525 @default.
• W2564652743 hasConceptScore W2564652743C501593827 @default.
• W2564652743 hasConceptScore W2564652743C547475151 @default.
• W2564652743 hasConceptScore W2564652743C55493867 @default.
• W2564652743 hasConceptScore W2564652743C71240020 @default.
• W2564652743 hasConceptScore W2564652743C8010536 @default.
• W2564652743 hasConceptScore W2564652743C86803240 @default.
• W2564652743 hasIssue "1" @default.
• W2564652743 hasLocation W25646527431 @default.
• W2564652743 hasLocation W25646527432 @default.
• W2564652743 hasOpenAccess W2564652743 @default.
• W2564652743 hasPrimaryLocation W25646527431 @default.
• W2564652743 hasRelatedWork W153595000 @default.
• W2564652743 hasRelatedWork W1933451099 @default.
• W2564652743 hasRelatedWork W1983880628 @default.
• W2564652743 hasRelatedWork W2059114757 @default.
• W2564652743 hasRelatedWork W2079484876 @default.
• W2564652743 hasRelatedWork W2106024571 @default.
• W2564652743 hasRelatedWork W2114362836 @default.
• W2564652743 hasRelatedWork W2119152808 @default.
• W2564652743 hasRelatedWork W2315403443 @default.
• W2564652743 hasRelatedWork W4361213549 @default.

• next