Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564759735> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2564759735 abstract "Mesenchymal stem cells (MSCs) have the potential to migrate to damaged tissues where they participate in tissue repair. Previous studies suggest that intravenous or intracortical injections of MSCs results in the emergence of binucleated cerebellar Purkinje cells (PCs) containing a MSC-derived marker protein in mice suggestive of a fusion event. However, transdifferentiation of MSCs into PCs or transfer of a marker protein from a MSC to a PC cannot be completely ruled-out. Here we unequivocally showed a fusion of human fetal MSCs (hMSCs) with mouse PCs by combination of tetracycline-regulated system with Cre-dependent genetic inversion switch (flip-excision; FLEx). We performed intra-cerebellar injection of adeno-associated viral vectors expressing tetracycline transactivator (tTA) and Cre recombinase into either non-symptomatic (4 week-old) or clearly symptomatic (6 – 8 month-old) spinocerebellar ataxia type 1 (SCA1) mice. Two weeks after the treatment, the mice received injection of 50,000 geneticially-engineered hMSCs that expressed GFP only in the presence of Cre recombinase and tTA, which restricted GFP expression exclusively to cerebellar cells fused with hMSCs. We observed GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice as early as 2 weeks after the MSC injection. The frequency of GFP-expressing PCs did not increase when analysed at longer time points (≈23 weeks post hMSC grafting), suggesting that most hMSC fusion events occurred within 2 weeks of delivery. Using this model, we show conclusively that hMSC display a tendency to fuse with degenerating PC and interneurons, and not with normal neurons." @default.
- W2564759735 created "2017-01-06" @default.
- W2564759735 creator A5001341469 @default.
- W2564759735 creator A5021310965 @default.
- W2564759735 creator A5026730026 @default.
- W2564759735 creator A5030659181 @default.
- W2564759735 creator A5039095558 @default.
- W2564759735 creator A5051911822 @default.
- W2564759735 creator A5073680568 @default.
- W2564759735 date "2015-05-01" @default.
- W2564759735 modified "2023-09-23" @default.
- W2564759735 title "598. Fusion of Mesenchymal Stem Cells Exclusively with “Degenerating” Cerebellar Neurons in Spinocerebellar Ataxia Type 1 Model Mice" @default.
- W2564759735 doi "https://doi.org/10.1016/s1525-0016(16)34207-1" @default.
- W2564759735 hasPublicationYear "2015" @default.
- W2564759735 type Work @default.
- W2564759735 sameAs 2564759735 @default.
- W2564759735 citedByCount "0" @default.
- W2564759735 crossrefType "journal-article" @default.
- W2564759735 hasAuthorship W2564759735A5001341469 @default.
- W2564759735 hasAuthorship W2564759735A5021310965 @default.
- W2564759735 hasAuthorship W2564759735A5026730026 @default.
- W2564759735 hasAuthorship W2564759735A5030659181 @default.
- W2564759735 hasAuthorship W2564759735A5039095558 @default.
- W2564759735 hasAuthorship W2564759735A5051911822 @default.
- W2564759735 hasAuthorship W2564759735A5073680568 @default.
- W2564759735 hasBestOaLocation W25647597351 @default.
- W2564759735 hasConcept C102230213 @default.
- W2564759735 hasConcept C104317684 @default.
- W2564759735 hasConcept C125929170 @default.
- W2564759735 hasConcept C141035611 @default.
- W2564759735 hasConcept C142613039 @default.
- W2564759735 hasConcept C142724271 @default.
- W2564759735 hasConcept C153911025 @default.
- W2564759735 hasConcept C169760540 @default.
- W2564759735 hasConcept C198826908 @default.
- W2564759735 hasConcept C2778107364 @default.
- W2564759735 hasConcept C2779652256 @default.
- W2564759735 hasConcept C28328180 @default.
- W2564759735 hasConcept C40767141 @default.
- W2564759735 hasConcept C54355233 @default.
- W2564759735 hasConcept C71924100 @default.
- W2564759735 hasConcept C86803240 @default.
- W2564759735 hasConcept C92087593 @default.
- W2564759735 hasConcept C95444343 @default.
- W2564759735 hasConceptScore W2564759735C102230213 @default.
- W2564759735 hasConceptScore W2564759735C104317684 @default.
- W2564759735 hasConceptScore W2564759735C125929170 @default.
- W2564759735 hasConceptScore W2564759735C141035611 @default.
- W2564759735 hasConceptScore W2564759735C142613039 @default.
- W2564759735 hasConceptScore W2564759735C142724271 @default.
- W2564759735 hasConceptScore W2564759735C153911025 @default.
- W2564759735 hasConceptScore W2564759735C169760540 @default.
- W2564759735 hasConceptScore W2564759735C198826908 @default.
- W2564759735 hasConceptScore W2564759735C2778107364 @default.
- W2564759735 hasConceptScore W2564759735C2779652256 @default.
- W2564759735 hasConceptScore W2564759735C28328180 @default.
- W2564759735 hasConceptScore W2564759735C40767141 @default.
- W2564759735 hasConceptScore W2564759735C54355233 @default.
- W2564759735 hasConceptScore W2564759735C71924100 @default.
- W2564759735 hasConceptScore W2564759735C86803240 @default.
- W2564759735 hasConceptScore W2564759735C92087593 @default.
- W2564759735 hasConceptScore W2564759735C95444343 @default.
- W2564759735 hasLocation W25647597351 @default.
- W2564759735 hasOpenAccess W2564759735 @default.
- W2564759735 hasPrimaryLocation W25647597351 @default.
- W2564759735 hasRelatedWork W1529040770 @default.
- W2564759735 hasRelatedWork W1964764355 @default.
- W2564759735 hasRelatedWork W1966009759 @default.
- W2564759735 hasRelatedWork W2003486896 @default.
- W2564759735 hasRelatedWork W2010482207 @default.
- W2564759735 hasRelatedWork W2056507657 @default.
- W2564759735 hasRelatedWork W2071731425 @default.
- W2564759735 hasRelatedWork W2088291566 @default.
- W2564759735 hasRelatedWork W2141178897 @default.
- W2564759735 hasRelatedWork W2354136247 @default.
- W2564759735 hasRelatedWork W2367085719 @default.
- W2564759735 hasRelatedWork W2374225092 @default.
- W2564759735 hasRelatedWork W2375490413 @default.
- W2564759735 hasRelatedWork W2378437130 @default.
- W2564759735 hasRelatedWork W2468114364 @default.
- W2564759735 hasRelatedWork W2536510277 @default.
- W2564759735 hasRelatedWork W2547137890 @default.
- W2564759735 hasRelatedWork W2589504032 @default.
- W2564759735 hasRelatedWork W797144263 @default.
- W2564759735 hasRelatedWork W2563069598 @default.
- W2564759735 isParatext "false" @default.
- W2564759735 isRetracted "false" @default.
- W2564759735 magId "2564759735" @default.
- W2564759735 workType "article" @default.