FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2564863154> ?p ?o ?g. }

• W2564863154 endingPage "1097" @default.
• W2564863154 startingPage "en.2016" @default.
• W2564863154 abstract "Uterine fibroids, or leiomyoma, are the most common benign tumors in women of reproductive age. In this work, the effect of silencing the mediator complex subunit 12 (Med12) gene in human uterine fibroid cells was evaluated. The role of Med12 in the modulation of Wnt/β-catenin and cell proliferation–associated signaling was evaluated in human uterine fibroid cells. Med12 was silenced in the immortalized human uterine fibroid cell line (HuLM) using a lentivirus-based Med12 gene–specific RNA interference strategy. HuLM cells were infected with lentiviruses carrying Med12-specific short hairpin RNA (shRNA) sequences or a nonfunctional shRNA scrambled control with green fluorescence protein. Stable cells that expressed low levels of Med12 protein were characterized. Wnt/β-catenin signaling, sex steroid receptor signaling, cell cycle–associated, and fibrosis-associated proteins were measured. Med12 knockdown cells showed significantly (P < 0.05) reduced levels of Wnt4 and β-catenin proteins as well as cell proliferation, as compared with scrambled control cells. Med12 knockdown cells also showed reduced levels of cell cycle–associated cyclin D1, Cdk1, and Cdk2 proteins as well as reduced activation of p-extracellular signal-regulated kinase, p-protein kinase B, and transforming growth factor (TGF)-β signaling pathways as compared with scrambled control cells. Moreover, TGF-β–regulated fibrosis-related proteins such as fibronectin, collagen type 1, and plasminogen activator inhibitor-1 were significantly (P < 0.05) reduced in Med12 knockdown cells as compared with scrambled control cells. Together, these results suggest that Med12 plays a key role in the regulation of HuLM cell proliferation through the modulation of Wnt/β-catenin, cell cycle–associated, and fibrosis-associated protein expression." @default.
• W2564863154 created "2017-01-06" @default.
• W2564863154 creator A5003343672 @default.
• W2564863154 creator A5005306051 @default.
• W2564863154 creator A5021004025 @default.
• W2564863154 creator A5027563231 @default.
• W2564863154 creator A5075577872 @default.
• W2564863154 creator A5091203148 @default.
• W2564863154 date "2016-12-14" @default.
• W2564863154 modified "2023-10-12" @default.
• W2564863154 title "Silencing Med12 Gene Reduces Proliferation of Human Leiomyoma Cells Mediated via Wnt/β-Catenin Signaling Pathway" @default.
• W2564863154 cites W1503498484 @default.
• W2564863154 cites W1526840795 @default.
• W2564863154 cites W1656358829 @default.
• W2564863154 cites W1879728393 @default.
• W2564863154 cites W1949181897 @default.
• W2564863154 cites W1984189354 @default.
• W2564863154 cites W1998401995 @default.
• W2564863154 cites W2000693350 @default.
• W2564863154 cites W2002697796 @default.
• W2564863154 cites W2008389846 @default.
• W2564863154 cites W2012248317 @default.
• W2564863154 cites W2014994159 @default.
• W2564863154 cites W2018529796 @default.
• W2564863154 cites W2024093804 @default.
• W2564863154 cites W2028151326 @default.
• W2564863154 cites W2033887719 @default.
• W2564863154 cites W2047839777 @default.
• W2564863154 cites W2049311981 @default.
• W2564863154 cites W2058174458 @default.
• W2564863154 cites W2066888185 @default.
• W2564863154 cites W2068249243 @default.
• W2564863154 cites W2083276959 @default.
• W2564863154 cites W2085357207 @default.
• W2564863154 cites W2086189048 @default.
• W2564863154 cites W2086692663 @default.
• W2564863154 cites W2091156943 @default.
• W2564863154 cites W2091455517 @default.
• W2564863154 cites W2091700826 @default.
• W2564863154 cites W2093612217 @default.
• W2564863154 cites W2100187878 @default.
• W2564863154 cites W2101222238 @default.
• W2564863154 cites W2102252943 @default.
• W2564863154 cites W2105594404 @default.
• W2564863154 cites W2107648723 @default.
• W2564863154 cites W2108124310 @default.
• W2564863154 cites W2125336329 @default.
• W2564863154 cites W2125649458 @default.
• W2564863154 cites W2127721080 @default.
• W2564863154 cites W2130383496 @default.
• W2564863154 cites W2131444472 @default.
• W2564863154 cites W2132152558 @default.
• W2564863154 cites W2133260209 @default.
• W2564863154 cites W2136644349 @default.
• W2564863154 cites W2137300752 @default.
• W2564863154 cites W2145992682 @default.
• W2564863154 cites W2146206232 @default.
• W2564863154 cites W2151583120 @default.
• W2564863154 cites W2157568141 @default.
• W2564863154 cites W2157600736 @default.
• W2564863154 cites W2157680871 @default.
• W2564863154 cites W2162081542 @default.
• W2564863154 cites W2327117722 @default.
• W2564863154 cites W2406630756 @default.
• W2564863154 cites W4240037437 @default.
• W2564863154 doi "https://doi.org/10.1210/en.2016-1097" @default.
• W2564863154 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5460776" @default.
• W2564863154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27967206" @default.
• W2564863154 hasPublicationYear "2016" @default.
• W2564863154 type Work @default.
• W2564863154 sameAs 2564863154 @default.
• W2564863154 citedByCount "38" @default.
• W2564863154 countsByYear W25648631542017 @default.
• W2564863154 countsByYear W25648631542018 @default.
• W2564863154 countsByYear W25648631542019 @default.
• W2564863154 countsByYear W25648631542020 @default.
• W2564863154 countsByYear W25648631542021 @default.
• W2564863154 countsByYear W25648631542022 @default.
• W2564863154 countsByYear W25648631542023 @default.
• W2564863154 crossrefType "journal-article" @default.
• W2564863154 hasAuthorship W2564863154A5003343672 @default.
• W2564863154 hasAuthorship W2564863154A5005306051 @default.
• W2564863154 hasAuthorship W2564863154A5021004025 @default.
• W2564863154 hasAuthorship W2564863154A5027563231 @default.
• W2564863154 hasAuthorship W2564863154A5075577872 @default.
• W2564863154 hasAuthorship W2564863154A5091203148 @default.
• W2564863154 hasBestOaLocation W25648631541 @default.
• W2564863154 hasConcept C137620995 @default.
• W2564863154 hasConcept C153911025 @default.
• W2564863154 hasConcept C173396325 @default.
• W2564863154 hasConcept C185592680 @default.
• W2564863154 hasConcept C190232843 @default.
• W2564863154 hasConcept C502942594 @default.
• W2564863154 hasConcept C54355233 @default.
• W2564863154 hasConcept C62112901 @default.
• W2564863154 hasConcept C62478195 @default.
• W2564863154 hasConcept C81885089 @default.
• W2564863154 hasConcept C86803240 @default.

• next