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- W2565065629 abstract "Rationale: Direct conversion or reprogramming of human postnatal cells into endothelial cells (ECs), bypassing stem or progenitor cell status, is crucial for regenerative medicine, cell therapy, and pathophysiological investigation but has remained largely unexplored. Objective: We sought to directly reprogram human postnatal dermal fibroblasts to ECs with vasculogenic and endothelial transcription factors and determine their vascularizing and therapeutic potential. Methods and Results: We utilized various combinations of 7 EC transcription factors to transduce human postnatal dermal fibroblasts and found that ER71/ETV2 (ETS variant 2) alone best induced endothelial features. KDR + (kinase insert domain receptor) cells sorted at day 7 from ER71/ETV2-transduced human postnatal dermal fibroblasts showed less mature but enriched endothelial characteristics and thus were referred to as early reprogrammed ECs (rECs), and did not undergo maturation by further culture. After a period of several weeks’ transgene-free culture followed by transient reinduction of ER71/ETV2, early rECs matured during 3 months of culture and showed reduced ETV2 expression, reaching a mature phenotype similar to postnatal human ECs. These were termed late rECs. While early rECs exhibited an immature phenotype, their implantation into ischemic hindlimbs induced enhanced recovery from ischemia. These 2 rECs showed clear capacity for contributing to new vessel formation through direct vascular incorporation in vivo. Paracrine or proangiogenic effects of implanted early rECs played a significant role in repairing hindlimb ischemia. Conclusions: This study for the first time demonstrates that ER71/ETV2 alone can directly reprogram human postnatal cells to functional, mature ECs after an intervening transgene-free period. These rECs could be valuable for cell therapy, personalized disease investigation, and exploration of the reprogramming process." @default.
- W2565065629 created "2017-01-06" @default.
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- W2565065629 date "2017-03-03" @default.
- W2565065629 modified "2023-10-11" @default.
- W2565065629 title "Direct Reprogramming of Human Dermal Fibroblasts Into Endothelial Cells Using ER71/ETV2" @default.
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- W2565065629 cites W1976212341 @default.
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- W2565065629 cites W1987448298 @default.
- W2565065629 cites W1989544046 @default.
- W2565065629 cites W1990014448 @default.
- W2565065629 cites W1991037959 @default.
- W2565065629 cites W1997134894 @default.
- W2565065629 cites W2000466286 @default.
- W2565065629 cites W2008241074 @default.
- W2565065629 cites W2008365696 @default.
- W2565065629 cites W2011237068 @default.
- W2565065629 cites W2011747072 @default.
- W2565065629 cites W2011849709 @default.
- W2565065629 cites W2021495513 @default.
- W2565065629 cites W2022507430 @default.
- W2565065629 cites W2030406511 @default.
- W2565065629 cites W2030414936 @default.
- W2565065629 cites W2032570745 @default.
- W2565065629 cites W2036109348 @default.
- W2565065629 cites W2038539380 @default.
- W2565065629 cites W2043167948 @default.
- W2565065629 cites W2047015004 @default.
- W2565065629 cites W2054066197 @default.
- W2565065629 cites W2056574134 @default.
- W2565065629 cites W2059038945 @default.
- W2565065629 cites W2059137671 @default.
- W2565065629 cites W2065115929 @default.
- W2565065629 cites W2065653704 @default.
- W2565065629 cites W2077640709 @default.
- W2565065629 cites W2082664445 @default.
- W2565065629 cites W2083134643 @default.
- W2565065629 cites W2093888082 @default.
- W2565065629 cites W2098597036 @default.
- W2565065629 cites W2103871063 @default.
- W2565065629 cites W2106921118 @default.
- W2565065629 cites W2109418446 @default.
- W2565065629 cites W2109972881 @default.
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- W2565065629 cites W2113086329 @default.
- W2565065629 cites W2119109414 @default.
- W2565065629 cites W2121288046 @default.
- W2565065629 cites W2122138696 @default.
- W2565065629 cites W2123955451 @default.
- W2565065629 cites W2124231777 @default.
- W2565065629 cites W2128141190 @default.
- W2565065629 cites W2128876415 @default.
- W2565065629 cites W2133346775 @default.
- W2565065629 cites W2136541538 @default.
- W2565065629 cites W2144907960 @default.
- W2565065629 cites W2152483372 @default.
- W2565065629 cites W2157893542 @default.
- W2565065629 cites W2160697532 @default.
- W2565065629 cites W2161322544 @default.
- W2565065629 cites W2163068618 @default.
- W2565065629 cites W2167202160 @default.
- W2565065629 cites W2172264076 @default.
- W2565065629 cites W2253553499 @default.
- W2565065629 cites W2380741403 @default.
- W2565065629 cites W2972045990 @default.
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- W2565065629 doi "https://doi.org/10.1161/circresaha.116.309833" @default.
- W2565065629 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5336520" @default.
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