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• W2565232452 abstract "Conclusion: Mesenchymal stem-cells are good candidates for cell-therapy of chronic tympanic membranes perforations.Objectives: To determine the effects of cell-based therapy in tympanic membrane perforations.Methods: Young C57BL/6 mice were anesthetized with intraperitoneal administration of ketamine and xylazine and randomly divided into three groups (n = 4 ears/group) that underwent bilateral sub-total pars tensa perforations of equal sizes using a sterile 27-gauge needle under a surgical microscope. Six-to-eight hours after injury, one group of mice did not receive treatment (acute perforation control), and the last two groups were treated with BM-MSCs embedded within HA scaffolds previously soaked in PBS to rinse culture media residues to avoid confounders and were euthanized 1 or 2 weeks after treatment.Results: Untreated tympanic membrane perforations developed a hyper-cellular infiltrate surrounding the injury site, while BM-MSC treated eardrums showed a reduced inflammatory response after the first week and a restoration of the trilaminar configuration 2 weeks after treatment, mimicking a normal tympanic membrane." @default.
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• W2565232452 date "2016-12-14" @default.
• W2565232452 modified "2023-10-06" @default.
• W2565232452 title "Histologic changes of mesenchymal stem cell repair of tympanic membrane perforation" @default.
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• W2565232452 doi "https://doi.org/10.1080/00016489.2016.1261411" @default.
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