FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2565446730> ?p ?o ?g. }

• W2565446730 abstract "Candidatus Liberibacter asiaticus (CLas), a non-culturable α-proteobacterium, is associated with citrus Huanglongbing (HLB, yellow shoot disease) currently threatening citrus production worldwide. Here, the whole genome sequence of CLas strain A4 from Guangdong of China was analyzed. Five copies of nrdB, encoding β-subunit of ribonucleotide reductase (RNR), a critical enzyme involving bacterial proliferation, were found. Three nrdB copies were in long form (nrdBL, 1,059 bp) and two were in short form (nrdBS, 378 bp). nrdBS shared >99% identity to 3' end of nrdBL and had no active site. Sequences of CLas nrdB genes formed a distinct monophyletic lineage among eubacteria. To make use of the high copy number feature, a nrdB-based primer set RNRf/RNRr was designed and evaluated using real-time PCR with 262 HLB samples collected from China and USA. Compared to the current standard primer set HLBas/HLBr derived from the 16S rRNA gene, RNRf/RNRr had Ct value reductions of 1.68 (SYBR Green PCR) and 1.77 (TaqMan PCR), thus increasing the detection sensitivity three-fold. Meanwhile, RNRf/RNRr was more than twice the stability of primer set LJ900f/LJ900r derived from multi-copy prophage. The nrdB-based PCR thereby provides a sensitive and reliable CLas detection with broad application, especially for the early diagnosis of HLB." @default.
• W2565446730 created "2017-01-06" @default.
• W2565446730 creator A5001463369 @default.
• W2565446730 creator A5011183162 @default.
• W2565446730 creator A5053491216 @default.
• W2565446730 creator A5053583417 @default.
• W2565446730 creator A5088888083 @default.
• W2565446730 creator A5090921557 @default.
• W2565446730 date "2016-12-01" @default.
• W2565446730 modified "2023-10-16" @default.
• W2565446730 title "Unusual Five Copies and Dual Forms of nrdB in “Candidatus Liberibacter asiaticus”: Biological Implications and PCR Detection Application" @default.
• W2565446730 cites W1568061161 @default.
• W2565446730 cites W1803102843 @default.
• W2565446730 cites W1984353977 @default.
• W2565446730 cites W1991194699 @default.
• W2565446730 cites W1994627492 @default.
• W2565446730 cites W2017694016 @default.
• W2565446730 cites W2032242297 @default.
• W2565446730 cites W2034632458 @default.
• W2565446730 cites W2068261242 @default.
• W2565446730 cites W2069128651 @default.
• W2565446730 cites W2079723706 @default.
• W2565446730 cites W2080233618 @default.
• W2565446730 cites W2080756776 @default.
• W2565446730 cites W2085972813 @default.
• W2565446730 cites W2118744932 @default.
• W2565446730 cites W2120894629 @default.
• W2565446730 cites W2126898257 @default.
• W2565446730 cites W2126899791 @default.
• W2565446730 cites W2127011204 @default.
• W2565446730 cites W2127322768 @default.
• W2565446730 cites W2132999105 @default.
• W2565446730 cites W2147330879 @default.
• W2565446730 cites W2149943094 @default.
• W2565446730 cites W2152207030 @default.
• W2565446730 cites W2159285197 @default.
• W2565446730 cites W2162098634 @default.
• W2565446730 cites W2165027548 @default.
• W2565446730 cites W2201015115 @default.
• W2565446730 cites W2225843978 @default.
• W2565446730 cites W4252954754 @default.
• W2565446730 cites W4256321774 @default.
• W2565446730 doi "https://doi.org/10.1038/srep39020" @default.
• W2565446730 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5154197" @default.
• W2565446730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27958354" @default.
• W2565446730 hasPublicationYear "2016" @default.
• W2565446730 type Work @default.
• W2565446730 sameAs 2565446730 @default.
• W2565446730 citedByCount "46" @default.
• W2565446730 countsByYear W25654467302017 @default.
• W2565446730 countsByYear W25654467302018 @default.
• W2565446730 countsByYear W25654467302019 @default.
• W2565446730 countsByYear W25654467302020 @default.
• W2565446730 countsByYear W25654467302021 @default.
• W2565446730 countsByYear W25654467302022 @default.
• W2565446730 countsByYear W25654467302023 @default.
• W2565446730 crossrefType "journal-article" @default.
• W2565446730 hasAuthorship W2565446730A5001463369 @default.
• W2565446730 hasAuthorship W2565446730A5011183162 @default.
• W2565446730 hasAuthorship W2565446730A5053491216 @default.
• W2565446730 hasAuthorship W2565446730A5053583417 @default.
• W2565446730 hasAuthorship W2565446730A5088888083 @default.
• W2565446730 hasAuthorship W2565446730A5090921557 @default.
• W2565446730 hasBestOaLocation W25654467301 @default.
• W2565446730 hasConcept C104317684 @default.
• W2565446730 hasConcept C143660080 @default.
• W2565446730 hasConcept C153911025 @default.
• W2565446730 hasConcept C178790620 @default.
• W2565446730 hasConcept C185592680 @default.
• W2565446730 hasConcept C2776441376 @default.
• W2565446730 hasConcept C2777563447 @default.
• W2565446730 hasConcept C42062724 @default.
• W2565446730 hasConcept C49105822 @default.
• W2565446730 hasConcept C54355233 @default.
• W2565446730 hasConcept C547475151 @default.
• W2565446730 hasConcept C73445445 @default.
• W2565446730 hasConcept C86803240 @default.
• W2565446730 hasConceptScore W2565446730C104317684 @default.
• W2565446730 hasConceptScore W2565446730C143660080 @default.
• W2565446730 hasConceptScore W2565446730C153911025 @default.
• W2565446730 hasConceptScore W2565446730C178790620 @default.
• W2565446730 hasConceptScore W2565446730C185592680 @default.
• W2565446730 hasConceptScore W2565446730C2776441376 @default.
• W2565446730 hasConceptScore W2565446730C2777563447 @default.
• W2565446730 hasConceptScore W2565446730C42062724 @default.
• W2565446730 hasConceptScore W2565446730C49105822 @default.
• W2565446730 hasConceptScore W2565446730C54355233 @default.
• W2565446730 hasConceptScore W2565446730C547475151 @default.
• W2565446730 hasConceptScore W2565446730C73445445 @default.
• W2565446730 hasConceptScore W2565446730C86803240 @default.
• W2565446730 hasIssue "1" @default.
• W2565446730 hasLocation W25654467301 @default.
• W2565446730 hasLocation W25654467302 @default.
• W2565446730 hasLocation W25654467303 @default.
• W2565446730 hasLocation W25654467304 @default.
• W2565446730 hasOpenAccess W2565446730 @default.
• W2565446730 hasPrimaryLocation W25654467301 @default.
• W2565446730 hasRelatedWork W1967467411 @default.
• W2565446730 hasRelatedWork W1979498904 @default.
• W2565446730 hasRelatedWork W1985154731 @default.

• next