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- W2565483734 abstract "Objective To investigate the roles of phosphatidylinositol 3-kinase(PI3K)/protein-serine-threonine kinases (Akt) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) signal transduction pathways in reduction of myocardial ischemia-reperfusion(I/R) injury by electric vagal stimulation postconditioning (EVSP),remote limb ischemic postconditioning (RLIP) or EVSP combined with RLIP (EVSP-RLIP) in rats.Methods Twenty male Sprague-Dawley rats,weighing 290-320 g,were randomly allocated into 4 groups (n=5 each):I/R group,EVSP group,RLIP group and EVSP-RLIP group.Myocardial I/R was induced by occlusion of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.In EVSP and EVSP-RLIP groups,the right vagus nerve was stimulated for 30 min with continuous electric rectangular pulses (2 ms,10 Hz,1-2 V) starting from 15 min of ischemia,and the voltage of the pulses was adjusted to decrease HR by 10％ of the baseline HR before stimulation.In RLIP and EVSP-RLIP groups,the animals underwent 10 min ischemia of bilateral hind limbs starting from 20 min of myocardial ischemia.The parameters of hemodynamics were recorded during I/R.At 60 min of reperfusion,the blood samples were taken from the jugular vein for determination of serum cardiac troponin I (cTnI) and creatine kinase MB (CK-MB) concentrations (by ELISA),and then the rats were sacrificed and myocardial specimens were obtained from both the ischemic and non-ischemic regions for determination of the levels of phosphorylated Akt (p-Akt) and phosphorylated STAT3 (p-STAT3) (by using Western blot) and expression of Akt and STAT3 mRNA (by RQ-PCR).Results Compared with I/R group,HR during I/R and serum cTnI and CK-MB concentrations were significantly decreased,and the levels of myocardial pAkt and p-STAT3 were increased in both ischemic and non-ischemic regions in EVSP,RLIP and EVSP-RLIP groups,and the expression of Akt and STAT3 mRNA in both ischemic and non-ischemic myocardial tissues was significantly increased in EVSP-RLIP group (P ＜ 0.05).Compared with EVSP or RLIP group,the serum CK-MB concentration was decreased,and the expression of myocardial Akt and STAT3 mRNA and levels of myocardial pAkt and p-STAT3 were significantly increased in both ischemic and non-ischemic regions in EVSP-RLIP group.Conclusion Activated PI3K/Akt and JAK/STAT signal transduction pathways mediate reduction of myocardial I/R injury by EVSP,RLIP or EVSP-RLIP in rats.Key words: Myocardial reperfusion injury; 1-Phosphatidylinositol 3-kinase; Protein-serine-threonine kinases ; STAT franscription factors ; Vagus nerve ; Electrical stimulation ; Hind limb ; Ischemic postconditioning" @default.
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- W2565483734 date "2013-08-20" @default.
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- W2565483734 title "Roles of PI3K/Akt and JAK/STAT signal transduction pathways in three different postconditionings-induced reduction of myocardial ischemia-reperfusion injury in rats" @default.
- W2565483734 doi "https://doi.org/10.3760/cma.j.issn.0254-1416.2013.08.024" @default.
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