Matches in SemOpenAlex for { <https://semopenalex.org/work/W2565577361> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2565577361 abstract "Streptomyces is the largest genus in the phylum Actinobacteria, known for their complex life cycle and secondary metabolite production. The model bacterium Streptomyces coelicolor grows _lamentously by tip extension and hyphal branching. The hyphal tip is highly organised, with various structural proteins forming a large assembly termed the polarisome. An essential protein, DivIVA, localises to the tips of the hyphae and has been shown to mark new branching sites. DivIVA has homologues in many Grampositive bacteria, and was shown to be essential for viability and direct polar growthin tested mycobacteria, corynebacteria and streptomycetes. DivIVA is always found at cell poles, but it is unclear how it is targeted to this subcellular location. Studies on B. subtilis DivIVA have proposed that it localises by sensing negative membrane curvature. Recently, it has been shown that S. coelicolor DivIVA is phosphorylated by the serine/threonine protein kinase AfsK. Overexpression of AfsK has dramatic e_ects on hyphal branching and DivIVA localisation in vivo. In this study, we try to set-up an experimental system to investigate the e_ects of phosphorylation on DivIVA and how it a_ects assembly of the protein on membrane surfaces in vitro. Native, phosphomimic and phosphorylated proteins were puri_ed and investigated by native gel electrophoresis and size exclusion chromatography. Fluorescent protein tagged versions were used for supported lipid bilayer binding experiments. We show that phosphorylation does not seem to have dramatic e_ects on oligomeric states of DivIVA, and that bilayer lipid composition is important in membrane binding. (Less)" @default.
- W2565577361 created "2017-01-06" @default.
- W2565577361 creator A5080306851 @default.
- W2565577361 date "2016-01-01" @default.
- W2565577361 modified "2023-09-27" @default.
- W2565577361 title "Effects of phosphorylation on oligomerisation and localisation of the Streptomyces coelicolor protein DivIVA in vitro" @default.
- W2565577361 hasPublicationYear "2016" @default.
- W2565577361 type Work @default.
- W2565577361 sameAs 2565577361 @default.
- W2565577361 citedByCount "0" @default.
- W2565577361 crossrefType "journal-article" @default.
- W2565577361 hasAuthorship W2565577361A5080306851 @default.
- W2565577361 hasConcept C12554922 @default.
- W2565577361 hasConcept C185592680 @default.
- W2565577361 hasConcept C2779920670 @default.
- W2565577361 hasConcept C2780681812 @default.
- W2565577361 hasConcept C39944091 @default.
- W2565577361 hasConcept C41625074 @default.
- W2565577361 hasConcept C523546767 @default.
- W2565577361 hasConcept C54355233 @default.
- W2565577361 hasConcept C55493867 @default.
- W2565577361 hasConcept C86803240 @default.
- W2565577361 hasConcept C95444343 @default.
- W2565577361 hasConceptScore W2565577361C12554922 @default.
- W2565577361 hasConceptScore W2565577361C185592680 @default.
- W2565577361 hasConceptScore W2565577361C2779920670 @default.
- W2565577361 hasConceptScore W2565577361C2780681812 @default.
- W2565577361 hasConceptScore W2565577361C39944091 @default.
- W2565577361 hasConceptScore W2565577361C41625074 @default.
- W2565577361 hasConceptScore W2565577361C523546767 @default.
- W2565577361 hasConceptScore W2565577361C54355233 @default.
- W2565577361 hasConceptScore W2565577361C55493867 @default.
- W2565577361 hasConceptScore W2565577361C86803240 @default.
- W2565577361 hasConceptScore W2565577361C95444343 @default.
- W2565577361 hasLocation W25655773611 @default.
- W2565577361 hasOpenAccess W2565577361 @default.
- W2565577361 hasPrimaryLocation W25655773611 @default.
- W2565577361 hasRelatedWork W1924144861 @default.
- W2565577361 hasRelatedWork W1990734927 @default.
- W2565577361 hasRelatedWork W2051748994 @default.
- W2565577361 hasRelatedWork W2070869861 @default.
- W2565577361 hasRelatedWork W2112285168 @default.
- W2565577361 hasRelatedWork W2120712416 @default.
- W2565577361 hasRelatedWork W2170719146 @default.
- W2565577361 hasRelatedWork W2317735273 @default.
- W2565577361 hasRelatedWork W2762338663 @default.
- W2565577361 hasRelatedWork W2896417296 @default.
- W2565577361 hasRelatedWork W2912767689 @default.
- W2565577361 hasRelatedWork W2960077179 @default.
- W2565577361 hasRelatedWork W2988612253 @default.
- W2565577361 hasRelatedWork W3004300058 @default.
- W2565577361 hasRelatedWork W3017175976 @default.
- W2565577361 hasRelatedWork W3044374485 @default.
- W2565577361 hasRelatedWork W3136670477 @default.
- W2565577361 hasRelatedWork W3164124043 @default.
- W2565577361 hasRelatedWork W3189369466 @default.
- W2565577361 hasRelatedWork W2511062391 @default.
- W2565577361 isParatext "false" @default.
- W2565577361 isRetracted "false" @default.
- W2565577361 magId "2565577361" @default.
- W2565577361 workType "article" @default.