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- W2565757444 abstract "There is growing interest to achieve tissue fabrication by designing the architecture of the substrate. Here we present a mesh culture approach which enables fabrication of standalone cell sheets by cell–cell contact mediated self-assembly organization on highly porous and microstructured SU-8 polymer meshes (apertures size >100 μm in length, mesh strands 3–5 μm in width), which are set suspended in a culture medium to limit cell-substrate interaction only to the fine mesh strands. We demonstrate that ingression of cells into the wide mesh openings proceeds solely by cell–cell adhesion, resulting in the formation of a uniform cell sheet over the highly porous mesh scaffolds. A uniformly spread actin meshwork dotted with reinforcement structures including transverse actin cables was present in cell sheets of fibroblasts while ring-like cables of actin/E-cadherin architecture was confirmed in epithelial cell sheets, suggesting the requirement for mechanically stable cell–cell adhesion architecture to overcome substrate adhesion limitations. Expression of type I collagen suggested the deposition of collagen and other extracellular matrices. Moreover, cell sheets could be maintained for an extended period of time (>100 d) without noticeable loss of cell viability, owing to improved exposure of the cell layer to medium in a suspension setup. Thus, the mesh culture offers an alternative method for extended cell culture and fabrication of standalone cell sheets for easy integration into microfluidics platforms for organ-on-chip studies." @default.
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- W2565757444 date "2016-12-19" @default.
- W2565757444 modified "2023-10-18" @default.
- W2565757444 title "Minimization of cell-substrate interaction using suspended microstructured meshes initiates cell sheet formation by self-assembly organization" @default.
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- W2565757444 doi "https://doi.org/10.1088/2057-1976/2/6/065019" @default.
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