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- W256576105 abstract "The molecular size of the neuronal, desipramine-sensitive and sodium-dependent noradrenaline carrier (NA-carrier) has been studied in PC12 rat phaeochromocytoma cells and bovine adreno-medullary membranes. Partial purification (by means of anion exchange and affinity chromatography) of the NA-carrier of digitonin-solubilized membranes of both tissues resulted in the enrichment of proteins characterized by a size of about 32 and 53 kilodalton (kd). In addition, 32 and 53 kd membrane proteins were covalently labelled when intact PC12 cells were incubated with the tritiated xylamine derivative 3 H-desmethyl xylamine ( 3 H-DMX). No labelling of these proteins was observed in the absence of sodium or in the presence an inhibitor of NA transport (e.g. nisoxetine) and in a NA transport-negative PC12 subclone. In bovine adreno-medullary membranes a 53 kd and a 40 kd but not a 32 kd protein was labelled by 3 H-DMX. The results indicate that the 53 kd protein may represent the NA-carrier." @default.
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- W256576105 date "1991-01-01" @default.
- W256576105 modified "2023-09-23" @default.
- W256576105 title "The Molecular Size of the Neuronal Noradrenaline Carrier" @default.
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- W256576105 doi "https://doi.org/10.1016/b978-0-08-041165-1.50053-3" @default.
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