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- W2565851825 abstract "Cells grown on flat two dimensional (2D) tissue culture substrates can differ considerably from the three dimensional (3D) physiological environment. There is growing evidence that tumor cell aggregates or cancer spheroids are more representative of tumors in vivo and they exhibit several physiological traits including similar morphology, the formation of cell-cell contacts, decreased proliferation, increased survival rates and a hypoxic core. However, variability in cancer spheroid culture has been a persistent problem for researchers. This variability is linked to medium composition and volume, cell density, duration in culture, and most importantly, the cellular interactions with the culture dish itself. Here, we demonstrate that a hydrophilic polymer coated surface, Thermo Scientific Nunclon Sphera, inhibits cell attachment to the culture dish by blocking binding of the extracellular matrix (ECM). The U bottom wells in the Nunclon Sphera plate provide the ideal size and shape for single spheroid formation in each well, offering consistency and reproducibility to the 3D tumor model system in vitro. The growth of cancer spheroids in the Nunclon Sphera 96U-well plate is superior to that of the non-treated plate with less “satellite colonies” and faster cell aggregation at low seeding density. Cancer cell viability and spheroid health are also evaluated by fluorescence- and colorimetric- based assays in the Nunclon Sphera plates. The Nunclon Sphera 96U-well plate provides an excellent solution for reproducibly generating uniform 3D cancer spheroids that are suitable for many cell-based drug discovery procedures, co-culture studies, and high-throughput drug screening. Citation Format: Cindy Neeley, Chetana M. Revankar. Formation of uniform and reproducible 3D cancer spheroids in a high throughput plate format. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 304. doi:10.1158/1538-7445.AM2015-304" @default.
- W2565851825 created "2017-01-06" @default.
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- W2565851825 date "2015-08-01" @default.
- W2565851825 modified "2023-09-27" @default.
- W2565851825 title "Abstract 304: Formation of uniform and reproducible 3D cancer spheroids in a high throughput plate format" @default.
- W2565851825 doi "https://doi.org/10.1158/1538-7445.am2015-304" @default.
- W2565851825 hasPublicationYear "2015" @default.
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