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• W2565963554 abstract "Abstract Pseudomonas aeruginosa, an opportunistic, but serious multidrug-resistant pathogen, secretes a ceramidase capable of cleaving the N -acyl linkage of ceramide to generate fatty acids and sphingosine. We previously reported that the secretion of P. aeruginosa ceramidase was induced by host-derived sphingolipids, through which phospholipase C-induced hemolysis was significantly enhanced. We herein investigated the gene(s) regulating sphingolipid-induced ceramidase expression and identified SphR, which encodes a putative AraC family transcriptional regulator. Disruption of the sphR gene in P. aeruginosa markedly decreased the sphingomyelin-induced secretion of ceramidase, reduced hemolytic activity, and resulted in the loss of sphingomyelin-induced ceramidase expression. A microarray analysis confirmed that sphingomyelin significantly induced ceramidase expression in P. aeruginosa . Furthermore, an electrophoretic mobility shift assay revealed that SphR specifically bound free sphingoid bases such as sphingosine, dihydrosphingosine, and phytosphingosine, but not sphingomyelin or ceramide. A β-galactosidase-assisted promoter assay showed that sphingosine activated ceramidase expression through SphR at a concentration of 100 nM. Collectively, these results demonstrated that sphingosine induces the secretion of ceramidase by promoting the mRNA expression of ceramidase through SphR, thereby enhancing hemolytic phospholipase C-induced cytotoxicity. These results facilitate understanding of the physiological role of bacterial ceramidase in host cells." @default.
• W2565963554 created "2017-01-06" @default.
• W2565963554 creator A5045942750 @default.
• W2565963554 creator A5085826947 @default.
• W2565963554 date "2016-12-12" @default.
• W2565963554 modified "2023-10-17" @default.
• W2565963554 title "Molecular mechanism for sphingosine-induced Pseudomonas ceramidase expression through the transcriptional regulator SphR" @default.
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• W2565963554 doi "https://doi.org/10.1038/srep38797" @default.
• W2565963554 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5150637" @default.
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• W2565963554 hasPublicationYear "2016" @default.
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