Matches in SemOpenAlex for { <https://semopenalex.org/work/W2566139987> ?p ?o ?g. }
- W2566139987 abstract "Extracellular matrix dynamics are key to tissue morphogenesis, homeostasis, injury, and repair. The spatiotemporal organization of this matrix has profound biological implications, but is challenging to monitor using standard techniques. Here, we address these challenges by using noncanonical amino acid tagging to fluorescently label extracellular matrix synthesized in the presence of bio-orthogonal methionine analogs. This strategy labels matrix proteins with high resolution, without compromising their distribution or mechanical function. We demonstrate that the organization and temporal dynamics of the proteinaceous matrix depend on the biophysical features of the microenvironment, including the biomaterial scaffold and the niche constructed by cells themselves. Pulse labeling experiments reveal that, in immature constructs, nascent matrix is highly fibrous and interdigitates with pre-existing matrix, while in more developed constructs, nascent matrix lacks fibrous organization and is retained in the immediate pericellular space. Inhibition of collagen crosslinking increases matrix synthesis, but compromises matrix organization. Finally, these data demonstrate marked cell-to-cell heterogeneity amongst both chondrocytes and mesenchymal stem cells undergoing chondrogenesis. Collectively, these results introduce fluorescent noncanonical amino acid tagging as a strategy to investigate spatiotemporal matrix organization, and demonstrate its ability to identify differences in phenotype, microenvironment, and matrix assembly at the single cell level." @default.
- W2566139987 created "2017-01-06" @default.
- W2566139987 creator A5013019941 @default.
- W2566139987 creator A5018983636 @default.
- W2566139987 date "2016-12-01" @default.
- W2566139987 modified "2023-10-12" @default.
- W2566139987 title "High fidelity visualization of cell-to-cell variation and temporal dynamics in nascent extracellular matrix formation" @default.
- W2566139987 cites W1574772177 @default.
- W2566139987 cites W1614928787 @default.
- W2566139987 cites W1971700271 @default.
- W2566139987 cites W1989433377 @default.
- W2566139987 cites W1989693137 @default.
- W2566139987 cites W1991903230 @default.
- W2566139987 cites W1996310883 @default.
- W2566139987 cites W2007736465 @default.
- W2566139987 cites W2013821696 @default.
- W2566139987 cites W2013868307 @default.
- W2566139987 cites W2015204889 @default.
- W2566139987 cites W2016849887 @default.
- W2566139987 cites W2023216474 @default.
- W2566139987 cites W2036395758 @default.
- W2566139987 cites W2042393267 @default.
- W2566139987 cites W2043877144 @default.
- W2566139987 cites W2051733665 @default.
- W2566139987 cites W2067132969 @default.
- W2566139987 cites W2069299659 @default.
- W2566139987 cites W2071485488 @default.
- W2566139987 cites W2084843851 @default.
- W2566139987 cites W2086898489 @default.
- W2566139987 cites W2089419574 @default.
- W2566139987 cites W2094996181 @default.
- W2566139987 cites W2099402486 @default.
- W2566139987 cites W2101336273 @default.
- W2566139987 cites W2102755926 @default.
- W2566139987 cites W2106316818 @default.
- W2566139987 cites W2107344789 @default.
- W2566139987 cites W2128057497 @default.
- W2566139987 cites W2128995541 @default.
- W2566139987 cites W2131205613 @default.
- W2566139987 cites W2134988306 @default.
- W2566139987 cites W2146385272 @default.
- W2566139987 cites W2166505869 @default.
- W2566139987 cites W2168970440 @default.
- W2566139987 cites W2260058559 @default.
- W2566139987 cites W2268470807 @default.
- W2566139987 cites W2268550346 @default.
- W2566139987 cites W2286717098 @default.
- W2566139987 cites W2291842205 @default.
- W2566139987 cites W2295897051 @default.
- W2566139987 cites W2410788784 @default.
- W2566139987 cites W2510034022 @default.
- W2566139987 cites W2739999456 @default.
- W2566139987 doi "https://doi.org/10.1038/srep38852" @default.
- W2566139987 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5150986" @default.
- W2566139987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27941914" @default.
- W2566139987 hasPublicationYear "2016" @default.
- W2566139987 type Work @default.
- W2566139987 sameAs 2566139987 @default.
- W2566139987 citedByCount "31" @default.
- W2566139987 countsByYear W25661399872017 @default.
- W2566139987 countsByYear W25661399872018 @default.
- W2566139987 countsByYear W25661399872019 @default.
- W2566139987 countsByYear W25661399872020 @default.
- W2566139987 countsByYear W25661399872021 @default.
- W2566139987 countsByYear W25661399872022 @default.
- W2566139987 countsByYear W25661399872023 @default.
- W2566139987 crossrefType "journal-article" @default.
- W2566139987 hasAuthorship W2566139987A5013019941 @default.
- W2566139987 hasAuthorship W2566139987A5018983636 @default.
- W2566139987 hasBestOaLocation W25661399871 @default.
- W2566139987 hasConcept C104317684 @default.
- W2566139987 hasConcept C106487976 @default.
- W2566139987 hasConcept C12554922 @default.
- W2566139987 hasConcept C1491633281 @default.
- W2566139987 hasConcept C15526883 @default.
- W2566139987 hasConcept C185592680 @default.
- W2566139987 hasConcept C189165786 @default.
- W2566139987 hasConcept C198826908 @default.
- W2566139987 hasConcept C2780171871 @default.
- W2566139987 hasConcept C40692019 @default.
- W2566139987 hasConcept C43617362 @default.
- W2566139987 hasConcept C55493867 @default.
- W2566139987 hasConcept C86803240 @default.
- W2566139987 hasConcept C95444343 @default.
- W2566139987 hasConceptScore W2566139987C104317684 @default.
- W2566139987 hasConceptScore W2566139987C106487976 @default.
- W2566139987 hasConceptScore W2566139987C12554922 @default.
- W2566139987 hasConceptScore W2566139987C1491633281 @default.
- W2566139987 hasConceptScore W2566139987C15526883 @default.
- W2566139987 hasConceptScore W2566139987C185592680 @default.
- W2566139987 hasConceptScore W2566139987C189165786 @default.
- W2566139987 hasConceptScore W2566139987C198826908 @default.
- W2566139987 hasConceptScore W2566139987C2780171871 @default.
- W2566139987 hasConceptScore W2566139987C40692019 @default.
- W2566139987 hasConceptScore W2566139987C43617362 @default.
- W2566139987 hasConceptScore W2566139987C55493867 @default.
- W2566139987 hasConceptScore W2566139987C86803240 @default.
- W2566139987 hasConceptScore W2566139987C95444343 @default.
- W2566139987 hasIssue "1" @default.
- W2566139987 hasLocation W25661399871 @default.