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- W2566476051 abstract "Some herpesviruses like human cytomegalovirus (HCMV) encode viral G protein-coupled receptors that cause reprogramming of cell signaling to facilitate dissemination of the virus, prevent immune surveillance and establish life-long latency. Human GPCRs are known to function in complex signaling networks involving direct physical interactions as well as indirect crosstalk of orthogonal signaling networks. The human chemokine receptor CXCR4 is expressed on hematopoietic stem cells, leukocytes, endothelial and epithelial cells, which are infected by HCMV or display reservoirs of latency. We investigated the potential heteromerization of US28 with CXCR4 as well as the influence of US28 on CXCR4 signaling. Using Bioluminescence Resonance Energy Transfer and luciferase-complementation based methods we show that US28 expression exhibits negative effects on CXCR4 signaling and constitutive surface expression in HEK293T cells. Furthermore, we demonstrate that this effect is not mediated by receptor heteromerization but via signaling crosstalk. Additionally, we show that in HCMV, strain TB40E, infected HUVEC the surface expression of CXCR4 is strongly downregulated, whereas in TB40E-delUS28 infected cells, CXCR4 surface expression is not altered in particular at late time points of infection. We show that the vGPCR US28 is leading to severely disturbed signaling and surface expression of the chemokine receptor CXCR4 thereby representing an effective mechanism used by vGPCRs to reprogram host cell signaling. In contrast to other studies, we demonstrate that these effects are not mediated via heteromerization." @default.
- W2566476051 created "2017-01-06" @default.
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- W2566476051 date "2016-12-01" @default.
- W2566476051 modified "2023-09-30" @default.
- W2566476051 title "Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28" @default.
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- W2566476051 cites W1961479051 @default.
- W2566476051 cites W1965223661 @default.
- W2566476051 cites W1965440699 @default.
- W2566476051 cites W1966113735 @default.
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- W2566476051 cites W1975644499 @default.
- W2566476051 cites W1981295860 @default.
- W2566476051 cites W1983213766 @default.
- W2566476051 cites W1986308794 @default.
- W2566476051 cites W1987042773 @default.
- W2566476051 cites W1990136945 @default.
- W2566476051 cites W1994637853 @default.
- W2566476051 cites W1995055452 @default.
- W2566476051 cites W1995153987 @default.
- W2566476051 cites W1995399236 @default.
- W2566476051 cites W2000343045 @default.
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- W2566476051 cites W2009089974 @default.
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- W2566476051 cites W2017214646 @default.
- W2566476051 cites W2023822910 @default.
- W2566476051 cites W2025024675 @default.
- W2566476051 cites W2026029378 @default.
- W2566476051 cites W2032612463 @default.
- W2566476051 cites W2035832696 @default.
- W2566476051 cites W2036276955 @default.
- W2566476051 cites W2037392106 @default.
- W2566476051 cites W2039754047 @default.
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- W2566476051 cites W2045367932 @default.
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- W2566476051 cites W2051057009 @default.
- W2566476051 cites W2071043013 @default.
- W2566476051 cites W2075400032 @default.
- W2566476051 cites W2085131300 @default.
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- W2566476051 cites W2093618199 @default.
- W2566476051 cites W2094592079 @default.
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- W2566476051 doi "https://doi.org/10.1186/s12964-016-0154-x" @default.
- W2566476051 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5153698" @default.
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