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• W2566766490 abstract "The objective was to determine how progesterone (P4) and FSH of slow release influences the quantity and quality of the oocytes collected by aspiration of the first follicular wave. The experiment was conducted in breeding season (33 o S, Uruguay) in ewes Merino Australian using the Day 0 Protocol for superstimulation of the first follicular wave (Menchaca et al., 2010, Reproduction Fertility and Development, Volume 22, pp 113-118). Intravaginal sponges of medroxiprogesterone acetate (Progespon, Syntex, Argentina) were used for 6 days associated with 125 ug of sodium cloprostenol (Ciclase DL, Syntex) and 200 UI eCG (Novormon, Syntex) given at sponges withdrawal and one dose of a GnRH analogue (8.4 mg of buserelin acetate, Receptal; Hoechst, Germany) was given 36 h after sponges withdrawal. This treatment ensure ovulation (Day 0) and the emergence of the first follicular wave around 72-84h after sponges withdrawal. The ewes were assigned to four experimental groups: Control Group (n=21); P4 Group (n=24) that received an intravaginal device with P4 (0.3 g, DICO, Syntex); FSH Group (n=16) treated with one dose of 80 mg FSHp (Folltropin, Bioniche Animal Health, Canada) reconstituted in sodium hyaluronic acid for slow release (10 ml, MAP-5, Bioniche Animal Health); and P4+FSH Group (n=14) that received the intravaginal device with P4 plus one dose of 80 mg FSHp in MAP-5 solvent. In both progesterone treated groups, the intravaginal devices were kept in place until laparoscopic follicular aspiration that was performed for all the females 72h after Day 0. Statistical analysis was performed by using ANOVA, Kruskall Wallis nonparametric test or chi square test. The treatment with P4 itself analysed as main factor did not affect the number of aspirated follicles (4.1 ± 0.7 vs. 3.8 ± 0.6) neither the number of collected oocytes (2.1 ± 0.7 vs. 1.9 ± 0.4) in comparison with no P4 treated females (P=NS). The treatment with FSH analysed as main effect increased the number of aspirated follicles (6.5±0.7 vs. 4.1±0.7; P<0.05) and the number of collected oocytes (3.4 ± 0.7 vs. 2.1 ± 0.7) in comparison with no FSH treated ewes. An interaction between treatments was observed (P<0,05), and the higher results were obtained in the P4+FSH group. The recovery rate and oocyte quality were not affected in any of the treatments (P=NS). In conclusion, the treatment with intravaginal P4 associated with one im dose of FSH in MAP-5 slow release solvent increases the follicular response and oocyte recovery when aspirating sheep at the first follicular wave." @default.
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• W2566766490 date "2015-03-01" @default.
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• W2566766490 title "Effect of the treatment with progesterone and FSH on follicular aspiration of the first follicular wave in ewes" @default.
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