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• W2566798082 abstract "We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools." @default.
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• W2566798082 date "2016-12-15" @default.
• W2566798082 modified "2023-09-26" @default.
• W2566798082 title "A Simple Way to Measure Alterations in Reward-seeking Behavior Using <em>Drosophila melanogaster</em>" @default.
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• W2566798082 doi "https://doi.org/10.3791/54910" @default.
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