FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2567133021> ?p ?o ?g. }

• W2567133021 abstract "Aptamers which bind cell surface receptors represent an exciting and potentially important class of ligands for the development of diagnostics and therapeutics. However, if aptamers are to be utilized in vivo, and more importantly for the targeted delivery of therapeutic cargoes, the molecular function must be robust, especially with regard to specificity and activity. Because assay formats for determining the binding specificity of aptamers to their cell surface targets vary greatly, and because some molecules have yet to be tested in vivo, it remains difficult to compare the function of these molecules and to identify which candidate molecules could be used for future development as both diagnostics and therapeutics. In order to assess this, we have chemically synthesized 15 different aptamers from the literature which target a range of human cell surface receptors associated with cancers. These include aptamers targeting EGFR, EPCAM, nucleolin, PSMA, PTK7 and hTfR, as well as some aptamers which have been derived from variations on ‘whole cell SELEX’ or similar approaches against cancer cells lines. Using standardized conditions, we assayed each aptamer in vitro using flow cytometry on a panel of different cancer cell lines to compare relative binding affinity and specificity. Molecules which function in vitro were subsequently assessed for tumor targeting capabilities in vivo using near-infrared imaging. Interestingly, some molecules which function robustly in vitro demonstrate little to no activity in vivo. Of the molecules tested,three demonstrate significant activity in vivo. These include an anti-EGFR aptamer, an anti-hTfR aptamer and a molecule identified via an ‘internalization selection method’ that is readily internalized by cancer cells, but shows little uptake by normal tissue. Taken as a whole, our data demonstrate some surprising differences in the apparent affinities and specificities of different aptamers when assayed under these standardized conditions. They also provide us with molecules which we can now think about transitioning for in vivo applications. Data on using these molecules to deliver cytotoxic drugs in vitro and in vivo will be presented." @default.
• W2567133021 created "2017-01-06" @default.
• W2567133021 creator A5017447019 @default.
• W2567133021 creator A5039254274 @default.
• W2567133021 creator A5084399310 @default.
• W2567133021 creator A5089602590 @default.
• W2567133021 date "2015-05-01" @default.
• W2567133021 modified "2023-09-22" @default.
• W2567133021 title "144. In Vivo Targeting hTfR and EGFR Using Aptamers" @default.
• W2567133021 doi "https://doi.org/10.1016/s1525-0016(16)33749-2" @default.
• W2567133021 hasPublicationYear "2015" @default.
• W2567133021 type Work @default.
• W2567133021 sameAs 2567133021 @default.
• W2567133021 citedByCount "0" @default.
• W2567133021 crossrefType "journal-article" @default.
• W2567133021 hasAuthorship W2567133021A5017447019 @default.
• W2567133021 hasAuthorship W2567133021A5039254274 @default.
• W2567133021 hasAuthorship W2567133021A5084399310 @default.
• W2567133021 hasAuthorship W2567133021A5089602590 @default.
• W2567133021 hasBestOaLocation W25671330211 @default.
• W2567133021 hasConcept C104317684 @default.
• W2567133021 hasConcept C122682173 @default.
• W2567133021 hasConcept C128972844 @default.
• W2567133021 hasConcept C139770010 @default.
• W2567133021 hasConcept C153911025 @default.
• W2567133021 hasConcept C170493617 @default.
• W2567133021 hasConcept C183873130 @default.
• W2567133021 hasConcept C185592680 @default.
• W2567133021 hasConcept C190062978 @default.
• W2567133021 hasConcept C202751555 @default.
• W2567133021 hasConcept C207001950 @default.
• W2567133021 hasConcept C2776525860 @default.
• W2567133021 hasConcept C54355233 @default.
• W2567133021 hasConcept C55493867 @default.
• W2567133021 hasConcept C67705224 @default.
• W2567133021 hasConcept C70721500 @default.
• W2567133021 hasConcept C86803240 @default.
• W2567133021 hasConcept C95444343 @default.
• W2567133021 hasConceptScore W2567133021C104317684 @default.
• W2567133021 hasConceptScore W2567133021C122682173 @default.
• W2567133021 hasConceptScore W2567133021C128972844 @default.
• W2567133021 hasConceptScore W2567133021C139770010 @default.
• W2567133021 hasConceptScore W2567133021C153911025 @default.
• W2567133021 hasConceptScore W2567133021C170493617 @default.
• W2567133021 hasConceptScore W2567133021C183873130 @default.
• W2567133021 hasConceptScore W2567133021C185592680 @default.
• W2567133021 hasConceptScore W2567133021C190062978 @default.
• W2567133021 hasConceptScore W2567133021C202751555 @default.
• W2567133021 hasConceptScore W2567133021C207001950 @default.
• W2567133021 hasConceptScore W2567133021C2776525860 @default.
• W2567133021 hasConceptScore W2567133021C54355233 @default.
• W2567133021 hasConceptScore W2567133021C55493867 @default.
• W2567133021 hasConceptScore W2567133021C67705224 @default.
• W2567133021 hasConceptScore W2567133021C70721500 @default.
• W2567133021 hasConceptScore W2567133021C86803240 @default.
• W2567133021 hasConceptScore W2567133021C95444343 @default.
• W2567133021 hasLocation W25671330211 @default.
• W2567133021 hasOpenAccess W2567133021 @default.
• W2567133021 hasPrimaryLocation W25671330211 @default.
• W2567133021 hasRelatedWork W1264225149 @default.
• W2567133021 hasRelatedWork W1499433071 @default.
• W2567133021 hasRelatedWork W1975749126 @default.
• W2567133021 hasRelatedWork W2054689345 @default.
• W2567133021 hasRelatedWork W2113091464 @default.
• W2567133021 hasRelatedWork W2136989828 @default.
• W2567133021 hasRelatedWork W2316090201 @default.
• W2567133021 hasRelatedWork W2360197077 @default.
• W2567133021 hasRelatedWork W2399530717 @default.
• W2567133021 hasRelatedWork W2403006369 @default.
• W2567133021 hasRelatedWork W2408956223 @default.
• W2567133021 hasRelatedWork W2464104723 @default.
• W2567133021 hasRelatedWork W2487617437 @default.
• W2567133021 hasRelatedWork W2564010196 @default.
• W2567133021 hasRelatedWork W2602653220 @default.
• W2567133021 hasRelatedWork W2695995371 @default.
• W2567133021 hasRelatedWork W3093374615 @default.
• W2567133021 hasRelatedWork W3209507060 @default.
• W2567133021 hasRelatedWork W2160188346 @default.
• W2567133021 hasRelatedWork W2515979287 @default.
• W2567133021 isParatext "false" @default.
• W2567133021 isRetracted "false" @default.
• W2567133021 magId "2567133021" @default.
• W2567133021 workType "article" @default.