FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2567432828> ?p ?o ?g. }

• W2567432828 abstract "Introduction. Lysosomal carboxypeptidase A [КE 3.4.17.1] is a zinc-dependent protease that participates actively at the terminal stages of proteolysis and regulation of metabolism of cells in many physiological and pathological processes. Purpose. To study biochemical properties of carboxypeptidase A from non-transformed tissue and benignant tumor tissue of the mammalian gland. Methods. Sampling of anatomical materials for research was conducted with compliance of ethical and legal standards. Excretion of the enzymes included gradual fractionation with the (NH 4 ) 2 SO 4 , dialysis in presence of 2.0 мМ Zn ++ and gel chromatography on the sephadex – G 75. The investigation of the substrate specificity of the enzymes was held by hydrolysis of the substrate of carbobenzoxyphenylalanine, phenylalanylalanine, glutamyltyrosine, prolylalanine (2.0 mM), haemoglobin and casein (2.0 %). The influence of inhibitors and activators was determined in presence of: DTT, Zn++, cysteine, triton X-100, soybean trypsin inhibitor, leupeptin, pepstatin, PHMB, FMSF, dimethylmolyemydanhydride, tozylheptanol, mercaptoethanol, EDTA and 1.10 - phenanthroline. The maximal velocity (V max ), Mihaelis constant (K m ), inhibition type and inhibition constant (K i ) analysed by Laynuiveru – Berku method. Results. Carboxypeptidase A from non-transformed tissue and benignant tumor of the mammalian gland better splits the substrates, which have hydrophobic and aromatic amino acids. The activity of carboxypeptidase A from the benignant tumor of the mammalian gland is inhibited most of all under influence of leupeptin, tozylheptanol and dimethylmolyemydanhydride, in contrast to non-transformed tissue. For carboxypeptidase A from non-transformed tissue of the mammalian gland K m = 0.24 mM and K i = 0.40 mM were determined, for carboxypeptidase A from the benignant tumor tissue of the mammalian gland – K m = 0.14 mM and K i = 0.16 mM. Conclusion. Carboxypeptidase A from non-transformed tissue and benignant tumor of the mammalian gland is identical as to the substrate specificity, inhibition by phenylalanine for noncompetitive type, inhibition and activation effect by reagent with the exclusion of leupeptin, tozylheptanol and dimethylmolyemydanhydride, but differ as to affinity to carbobenzoxyphenylalanine and sensitivity to phenylalanine." @default.
• W2567432828 created "2017-01-06" @default.
• W2567432828 creator A5043118343 @default.
• W2567432828 creator A5070857650 @default.
• W2567432828 date "2016-11-10" @default.
• W2567432828 modified "2023-10-17" @default.
• W2567432828 title "BIOCHEMICAL PROPERTIES OF CARBOXYPEPTIDASE A FROM NON-TRANSFERRED TISSUE AND BENIGNANT TUMOR TISSUE OF THE MAMMALIAN GLAND" @default.
• W2567432828 cites W119728110 @default.
• W2567432828 cites W1577234842 @default.
• W2567432828 cites W1775749144 @default.
• W2567432828 cites W17844780 @default.
• W2567432828 cites W1876705010 @default.
• W2567432828 cites W1966870640 @default.
• W2567432828 cites W1972300130 @default.
• W2567432828 cites W1985228636 @default.
• W2567432828 cites W1985788605 @default.
• W2567432828 cites W1988517119 @default.
• W2567432828 cites W2006277507 @default.
• W2567432828 cites W2013319890 @default.
• W2567432828 cites W2026793582 @default.
• W2567432828 cites W2038214310 @default.
• W2567432828 cites W2045886156 @default.
• W2567432828 cites W2046497890 @default.
• W2567432828 cites W2059833587 @default.
• W2567432828 cites W2060736718 @default.
• W2567432828 cites W2060948669 @default.
• W2567432828 cites W2068858849 @default.
• W2567432828 cites W2108450409 @default.
• W2567432828 cites W2118303162 @default.
• W2567432828 cites W2124745118 @default.
• W2567432828 cites W2129385510 @default.
• W2567432828 cites W2136851205 @default.
• W2567432828 cites W2151297171 @default.
• W2567432828 cites W2152782018 @default.
• W2567432828 cites W2402505366 @default.
• W2567432828 cites W68504299 @default.
• W2567432828 doi "https://doi.org/10.18524/2077-1746.2016.2(39).82744" @default.
• W2567432828 hasPublicationYear "2016" @default.
• W2567432828 type Work @default.
• W2567432828 sameAs 2567432828 @default.
• W2567432828 citedByCount "0" @default.
• W2567432828 crossrefType "journal-article" @default.
• W2567432828 hasAuthorship W2567432828A5043118343 @default.
• W2567432828 hasAuthorship W2567432828A5070857650 @default.
• W2567432828 hasBestOaLocation W25674328281 @default.
• W2567432828 hasConcept C108477463 @default.
• W2567432828 hasConcept C136398272 @default.
• W2567432828 hasConcept C181199279 @default.
• W2567432828 hasConcept C185592680 @default.
• W2567432828 hasConcept C2775883766 @default.
• W2567432828 hasConcept C2776350231 @default.
• W2567432828 hasConcept C2776714187 @default.
• W2567432828 hasConcept C2777774944 @default.
• W2567432828 hasConcept C2780327651 @default.
• W2567432828 hasConcept C2780340462 @default.
• W2567432828 hasConcept C2780912031 @default.
• W2567432828 hasConcept C515207424 @default.
• W2567432828 hasConcept C55493867 @default.
• W2567432828 hasConceptScore W2567432828C108477463 @default.
• W2567432828 hasConceptScore W2567432828C136398272 @default.
• W2567432828 hasConceptScore W2567432828C181199279 @default.
• W2567432828 hasConceptScore W2567432828C185592680 @default.
• W2567432828 hasConceptScore W2567432828C2775883766 @default.
• W2567432828 hasConceptScore W2567432828C2776350231 @default.
• W2567432828 hasConceptScore W2567432828C2776714187 @default.
• W2567432828 hasConceptScore W2567432828C2777774944 @default.
• W2567432828 hasConceptScore W2567432828C2780327651 @default.
• W2567432828 hasConceptScore W2567432828C2780340462 @default.
• W2567432828 hasConceptScore W2567432828C2780912031 @default.
• W2567432828 hasConceptScore W2567432828C515207424 @default.
• W2567432828 hasConceptScore W2567432828C55493867 @default.
• W2567432828 hasLocation W25674328281 @default.
• W2567432828 hasOpenAccess W2567432828 @default.
• W2567432828 hasPrimaryLocation W25674328281 @default.
• W2567432828 hasRelatedWork W149090166 @default.
• W2567432828 hasRelatedWork W1518773224 @default.
• W2567432828 hasRelatedWork W1584663923 @default.
• W2567432828 hasRelatedWork W1605024684 @default.
• W2567432828 hasRelatedWork W1904560112 @default.
• W2567432828 hasRelatedWork W1966053638 @default.
• W2567432828 hasRelatedWork W1996752024 @default.
• W2567432828 hasRelatedWork W2011921956 @default.
• W2567432828 hasRelatedWork W2019509866 @default.
• W2567432828 hasRelatedWork W2068886163 @default.
• W2567432828 hasRelatedWork W2087316679 @default.
• W2567432828 hasRelatedWork W2091893175 @default.
• W2567432828 hasRelatedWork W2356070922 @default.
• W2567432828 hasRelatedWork W2407977287 @default.
• W2567432828 hasRelatedWork W2409252021 @default.
• W2567432828 hasRelatedWork W2410970063 @default.
• W2567432828 hasRelatedWork W2414225150 @default.
• W2567432828 hasRelatedWork W2414626347 @default.
• W2567432828 hasRelatedWork W2416558825 @default.
• W2567432828 hasRelatedWork W2432596285 @default.
• W2567432828 isParatext "false" @default.
• W2567432828 isRetracted "false" @default.
• W2567432828 magId "2567432828" @default.
• W2567432828 workType "article" @default.