FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2567553314> ?p ?o ?g. }

• W2567553314 endingPage "3030" @default.
• W2567553314 startingPage "3017" @default.
• W2567553314 abstract "Enhancers are intergenic DNA elements that regulate the transcription of target genes in response to signaling pathways by interacting with promoters over large genomic distances. Recent studies have revealed that enhancers are bi-directionally transcribed into enhancer RNAs (eRNAs). Using single-molecule fluorescence in situ hybridization (smFISH), we investigated the eRNA-mediated regulation of transcription during estrogen induction in MCF-7 cells. We demonstrate that eRNAs are localized exclusively in the nucleus and are induced with similar kinetics as target mRNAs. However, eRNAs are mostly nascent at enhancers and their steady-state levels remain lower than those of their cognate mRNAs. Surprisingly, at the single-allele level, eRNAs are rarely co-expressed with their target loci, demonstrating that active gene transcription does not require the continuous transcription of eRNAs or their accumulation at enhancers. When co-expressed, sub-diffraction distance measurements between nascent mRNA and eRNA signals reveal that co-transcription of eRNAs and mRNAs rarely occurs within closed enhancer-promoter loops. Lastly, basal eRNA transcription at enhancers, but not E2-induced transcription, is maintained upon depletion of MLL1 and ERα, suggesting some degree of chromatin accessibility prior to signal-dependent activation of transcription. Together, our findings suggest that eRNA accumulation at enhancer-promoter loops is not required to sustain target gene transcription." @default.
• W2567553314 created "2017-01-06" @default.
• W2567553314 creator A5012750505 @default.
• W2567553314 creator A5020001679 @default.
• W2567553314 creator A5063813876 @default.
• W2567553314 creator A5065261532 @default.
• W2567553314 creator A5069740493 @default.
• W2567553314 creator A5070661197 @default.
• W2567553314 creator A5091666269 @default.
• W2567553314 date "2016-12-08" @default.
• W2567553314 modified "2023-10-06" @default.
• W2567553314 title "Single-cell profiling reveals that eRNA accumulation at enhancer–promoter loops is not required to sustain transcription" @default.
• W2567553314 cites W1625536075 @default.
• W2567553314 cites W1863874155 @default.
• W2567553314 cites W1970831058 @default.
• W2567553314 cites W1976714996 @default.
• W2567553314 cites W1978901723 @default.
• W2567553314 cites W1985105928 @default.
• W2567553314 cites W1992927069 @default.
• W2567553314 cites W1995226230 @default.
• W2567553314 cites W1996060670 @default.
• W2567553314 cites W1998871958 @default.
• W2567553314 cites W2005361347 @default.
• W2567553314 cites W2006936482 @default.
• W2567553314 cites W2008227324 @default.
• W2567553314 cites W2014787267 @default.
• W2567553314 cites W2015104432 @default.
• W2567553314 cites W2015845481 @default.
• W2567553314 cites W2022993325 @default.
• W2567553314 cites W2023095055 @default.
• W2567553314 cites W2039576757 @default.
• W2567553314 cites W2053293102 @default.
• W2567553314 cites W2061275528 @default.
• W2567553314 cites W2062552264 @default.
• W2567553314 cites W2064538303 @default.
• W2567553314 cites W2072822958 @default.
• W2567553314 cites W2074915491 @default.
• W2567553314 cites W2078910228 @default.
• W2567553314 cites W2082161548 @default.
• W2567553314 cites W2082832087 @default.
• W2567553314 cites W2090919497 @default.
• W2567553314 cites W2094468530 @default.
• W2567553314 cites W2096010243 @default.
• W2567553314 cites W2103354991 @default.
• W2567553314 cites W2105512180 @default.
• W2567553314 cites W2111099379 @default.
• W2567553314 cites W2113945915 @default.
• W2567553314 cites W2116829860 @default.
• W2567553314 cites W2118532317 @default.
• W2567553314 cites W2121094803 @default.
• W2567553314 cites W2121659981 @default.
• W2567553314 cites W2132257972 @default.
• W2567553314 cites W2141688036 @default.
• W2567553314 cites W2149475194 @default.
• W2567553314 cites W2150698576 @default.
• W2567553314 cites W2152144700 @default.
• W2567553314 cites W2154746182 @default.
• W2567553314 cites W2161797274 @default.
• W2567553314 cites W2163098987 @default.
• W2567553314 cites W2166037178 @default.
• W2567553314 cites W2168602111 @default.
• W2567553314 cites W2169389191 @default.
• W2567553314 cites W2618657542 @default.
• W2567553314 cites W4256266823 @default.
• W2567553314 doi "https://doi.org/10.1093/nar/gkw1220" @default.
• W2567553314 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5389544" @default.
• W2567553314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27932455" @default.
• W2567553314 hasPublicationYear "2016" @default.
• W2567553314 type Work @default.
• W2567553314 sameAs 2567553314 @default.
• W2567553314 citedByCount "46" @default.
• W2567553314 countsByYear W25675533142017 @default.
• W2567553314 countsByYear W25675533142018 @default.
• W2567553314 countsByYear W25675533142019 @default.
• W2567553314 countsByYear W25675533142020 @default.
• W2567553314 countsByYear W25675533142021 @default.
• W2567553314 countsByYear W25675533142022 @default.
• W2567553314 countsByYear W25675533142023 @default.
• W2567553314 crossrefType "journal-article" @default.
• W2567553314 hasAuthorship W2567553314A5012750505 @default.
• W2567553314 hasAuthorship W2567553314A5020001679 @default.
• W2567553314 hasAuthorship W2567553314A5063813876 @default.
• W2567553314 hasAuthorship W2567553314A5065261532 @default.
• W2567553314 hasAuthorship W2567553314A5069740493 @default.
• W2567553314 hasAuthorship W2567553314A5070661197 @default.
• W2567553314 hasAuthorship W2567553314A5091666269 @default.
• W2567553314 hasBestOaLocation W25675533141 @default.
• W2567553314 hasConcept C101762097 @default.
• W2567553314 hasConcept C104317684 @default.
• W2567553314 hasConcept C111936080 @default.
• W2567553314 hasConcept C138885662 @default.
• W2567553314 hasConcept C144825837 @default.
• W2567553314 hasConcept C150194340 @default.
• W2567553314 hasConcept C153911025 @default.
• W2567553314 hasConcept C165864922 @default.
• W2567553314 hasConcept C179926584 @default.
• W2567553314 hasConcept C21784809 @default.
• W2567553314 hasConcept C41895202 @default.

• next