Matches in SemOpenAlex for { <https://semopenalex.org/work/W2567664386> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2567664386 abstract "Abstract S-phase kinase-associated protein 2 (SKP-2) is a proto-oncogene that has been shown to be expressed in a number of tumors. A number of studies have shown that SKP-2 plays a role in the degradation of tumor suppressor genes by increased proteosome-dependent degradation. SKP-2 overexpression is highly representative of intrinsic biological aggressiveness of certain cancers including breast, non-small cell lung cancer and gastric carcinoma, how ever its role in hematological malignancies have not yet been explored. Therefore, in this study we examined 100 clinical samples of diffuse large B-cell lymphoma (DLBCL) to study the expression pattern of ubiquitin ligase subunit SKP-2 proto-oncogenes and its relation to proliferative index marker protein Ki67 to correlate tumor aggressiveness. The expression of SKP-2 and Ki67 were examined by immunohistochemistry using specific antibodies on formalin-embedded tissue sections of DLBCL patients. Our data showed that SKP-2 was over expressed in majority of DLBCL and was associated with expression pattern of the proliferating index marker Ki67 protein. Since increased proteosome-dependent degradation of tumor suppressor genes play a critical role in the etiology of various tumors and proteosome inhibition is a novel approach for treating malignancies and has been approved for clinical use, we sought to determine whether inhibition of proteosome by MG132, a specific proteosome inhibitor induces apoptosis in a panel of DLBCL cell lines. Our data showed that treatment of DLBCL cell lines by MG132 induced apoptosis in a dose dependent manner. Inhibition of proteosome also decreased the expression of SKP-2 leading to subsequent disruption of mitochondrial membrane potential causing release of cytochrome c into the cytosol. Release of cytochrome c resulted in activation of caspase-3 and cleavage of PARP ultimately leading to apoptosis. These data suggests that SKP-2 expression plays a major role in the oncogenesis of DLBCL and overexpression of SKP-2 can be used as useful prognostic marker. Furthermore, proteosome inhibitors can be used as future therapeutic modality in treating DLBCL." @default.
- W2567664386 created "2017-01-06" @default.
- W2567664386 creator A5012192417 @default.
- W2567664386 creator A5015528853 @default.
- W2567664386 creator A5018975121 @default.
- W2567664386 creator A5055366985 @default.
- W2567664386 date "2006-11-16" @default.
- W2567664386 modified "2023-09-28" @default.
- W2567664386 title "Significance of Ubiquitin Ligase Subunit SKP-2 Proto-Oncogene and Proliferative Marker Ki67 Expression in Diffuse Large B-Cell Lymphoma." @default.
- W2567664386 doi "https://doi.org/10.1182/blood.v108.11.4343.4343" @default.
- W2567664386 hasPublicationYear "2006" @default.
- W2567664386 type Work @default.
- W2567664386 sameAs 2567664386 @default.
- W2567664386 citedByCount "0" @default.
- W2567664386 crossrefType "journal-article" @default.
- W2567664386 hasAuthorship W2567664386A5012192417 @default.
- W2567664386 hasAuthorship W2567664386A5015528853 @default.
- W2567664386 hasAuthorship W2567664386A5018975121 @default.
- W2567664386 hasAuthorship W2567664386A5055366985 @default.
- W2567664386 hasConcept C104317684 @default.
- W2567664386 hasConcept C121608353 @default.
- W2567664386 hasConcept C134459356 @default.
- W2567664386 hasConcept C203014093 @default.
- W2567664386 hasConcept C25602115 @default.
- W2567664386 hasConcept C27740335 @default.
- W2567664386 hasConcept C2778367456 @default.
- W2567664386 hasConcept C2778506107 @default.
- W2567664386 hasConcept C2778559949 @default.
- W2567664386 hasConcept C2779338263 @default.
- W2567664386 hasConcept C2781018059 @default.
- W2567664386 hasConcept C29537977 @default.
- W2567664386 hasConcept C2992195973 @default.
- W2567664386 hasConcept C502942594 @default.
- W2567664386 hasConcept C54355233 @default.
- W2567664386 hasConcept C86803240 @default.
- W2567664386 hasConcept C95444343 @default.
- W2567664386 hasConceptScore W2567664386C104317684 @default.
- W2567664386 hasConceptScore W2567664386C121608353 @default.
- W2567664386 hasConceptScore W2567664386C134459356 @default.
- W2567664386 hasConceptScore W2567664386C203014093 @default.
- W2567664386 hasConceptScore W2567664386C25602115 @default.
- W2567664386 hasConceptScore W2567664386C27740335 @default.
- W2567664386 hasConceptScore W2567664386C2778367456 @default.
- W2567664386 hasConceptScore W2567664386C2778506107 @default.
- W2567664386 hasConceptScore W2567664386C2778559949 @default.
- W2567664386 hasConceptScore W2567664386C2779338263 @default.
- W2567664386 hasConceptScore W2567664386C2781018059 @default.
- W2567664386 hasConceptScore W2567664386C29537977 @default.
- W2567664386 hasConceptScore W2567664386C2992195973 @default.
- W2567664386 hasConceptScore W2567664386C502942594 @default.
- W2567664386 hasConceptScore W2567664386C54355233 @default.
- W2567664386 hasConceptScore W2567664386C86803240 @default.
- W2567664386 hasConceptScore W2567664386C95444343 @default.
- W2567664386 hasLocation W25676643861 @default.
- W2567664386 hasOpenAccess W2567664386 @default.
- W2567664386 hasPrimaryLocation W25676643861 @default.
- W2567664386 hasRelatedWork W2004372671 @default.
- W2567664386 hasRelatedWork W2004614920 @default.
- W2567664386 hasRelatedWork W2012418553 @default.
- W2567664386 hasRelatedWork W2019341167 @default.
- W2567664386 hasRelatedWork W2080983445 @default.
- W2567664386 hasRelatedWork W2092541317 @default.
- W2567664386 hasRelatedWork W2165531958 @default.
- W2567664386 hasRelatedWork W2567664386 @default.
- W2567664386 hasRelatedWork W2900917031 @default.
- W2567664386 hasRelatedWork W2910722456 @default.
- W2567664386 isParatext "false" @default.
- W2567664386 isRetracted "false" @default.
- W2567664386 magId "2567664386" @default.
- W2567664386 workType "article" @default.