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- W2567802170 abstract "Recently, studies of positional relationships of cells are seemed to be very important for cell biology. We proposed a novel concept that self-organized vasculatum integrates artificial orifices on a device by VEGF (Vascular Endothelial Growth Factor), and a method of uniformly plating HUVEC (Human Umbilical Vein Endothelial Cells) for efficient vasculature integration. Sucking and clamping of Alginate Calcium gel beads containing VEGF, it is able to induce angiogenesis to orifices on embedded channels. Endothelial cells characteristically organize vasculature by themselves in collagen gel and grow along the VEGF concentration gradient. HUVEC were cultured on device for about 7 days in the collagen gel and artificial vasculature mere immobilized on orifices by self-organized. And viscosity control of the suspension mixed with HUVEC, collagen gel and medium at low temperature made HUVEC uniformly-plated." @default.
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- W2567802170 date "2008-01-01" @default.
- W2567802170 modified "2023-09-23" @default.
- W2567802170 title "319 Fluid introduction into vasculature using MEMS device" @default.
- W2567802170 doi "https://doi.org/10.1299/jsmemecjo.2008.8.0_37" @default.
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