Matches in SemOpenAlex for { <https://semopenalex.org/work/W2568763370> ?p ?o ?g. }
- W2568763370 endingPage "1468" @default.
- W2568763370 startingPage "1459" @default.
- W2568763370 abstract "Mass spectrometry (MS) has become an indispensable tool for investigating the architectures and dynamics of macromolecular assemblies. Here we show that covalent labeling of solvent accessible residues followed by their MS-based identification yields modeling restraints that allow mapping the location and orientation of subunits within protein assemblies. Together with complementary restraints derived from cross-linking and native MS, we built native-like models of four heterocomplexes with known subunit structures and compared them with available X-ray crystal structures. The results demonstrated that covalent labeling followed by MS markedly increased the predictive power of the integrative modeling strategy enabling more accurate protein assembly models. We applied this strategy to the F-type ATP synthase from spinach chloroplasts (cATPase) providing a structural basis for its function as a nanomotor. By subjecting the models generated by our restraint-based strategy to molecular dynamics (MD) simulations, we revealed the conformational states of the peripheral stalk and assigned flexible regions in the enzyme. Our strategy can readily incorporate complementary chemical labeling strategies and we anticipate that it will be applicable to many other systems providing new insights into the structure and function of protein complexes." @default.
- W2568763370 created "2017-01-13" @default.
- W2568763370 creator A5049119135 @default.
- W2568763370 creator A5065901458 @default.
- W2568763370 creator A5067412023 @default.
- W2568763370 creator A5068723426 @default.
- W2568763370 creator A5087568097 @default.
- W2568763370 creator A5090735369 @default.
- W2568763370 date "2017-01-18" @default.
- W2568763370 modified "2023-10-16" @default.
- W2568763370 title "Surface Accessibility and Dynamics of Macromolecular Assemblies Probed by Covalent Labeling Mass Spectrometry and Integrative Modeling" @default.
- W2568763370 cites W1964013536 @default.
- W2568763370 cites W1964051078 @default.
- W2568763370 cites W1964333133 @default.
- W2568763370 cites W1966078827 @default.
- W2568763370 cites W1967086556 @default.
- W2568763370 cites W1967317016 @default.
- W2568763370 cites W1970954652 @default.
- W2568763370 cites W1971795378 @default.
- W2568763370 cites W1973739970 @default.
- W2568763370 cites W1976499671 @default.
- W2568763370 cites W1978496023 @default.
- W2568763370 cites W1981335059 @default.
- W2568763370 cites W1983995089 @default.
- W2568763370 cites W1989790643 @default.
- W2568763370 cites W1990416959 @default.
- W2568763370 cites W1996420077 @default.
- W2568763370 cites W2003427751 @default.
- W2568763370 cites W2007188470 @default.
- W2568763370 cites W2007477408 @default.
- W2568763370 cites W2018871138 @default.
- W2568763370 cites W2018911969 @default.
- W2568763370 cites W2024849484 @default.
- W2568763370 cites W2026507477 @default.
- W2568763370 cites W2027849052 @default.
- W2568763370 cites W2028257597 @default.
- W2568763370 cites W2029667189 @default.
- W2568763370 cites W2033300775 @default.
- W2568763370 cites W2035266068 @default.
- W2568763370 cites W2041077593 @default.
- W2568763370 cites W2046164706 @default.
- W2568763370 cites W2052618584 @default.
- W2568763370 cites W2057020790 @default.
- W2568763370 cites W2057477511 @default.
- W2568763370 cites W2064980587 @default.
- W2568763370 cites W2065283382 @default.
- W2568763370 cites W2067174909 @default.
- W2568763370 cites W2067800709 @default.
- W2568763370 cites W2068722030 @default.
- W2568763370 cites W2071950147 @default.
- W2568763370 cites W2082419458 @default.
- W2568763370 cites W2083157472 @default.
- W2568763370 cites W2085485834 @default.
- W2568763370 cites W2086145691 @default.
- W2568763370 cites W2090615391 @default.
- W2568763370 cites W2091868307 @default.
- W2568763370 cites W2103647536 @default.
- W2568763370 cites W2103945336 @default.
- W2568763370 cites W2104611886 @default.
- W2568763370 cites W2111527039 @default.
- W2568763370 cites W2113579040 @default.
- W2568763370 cites W2115463031 @default.
- W2568763370 cites W2128053367 @default.
- W2568763370 cites W2129733824 @default.
- W2568763370 cites W2134749398 @default.
- W2568763370 cites W2139413101 @default.
- W2568763370 cites W2146349416 @default.
- W2568763370 cites W2147024375 @default.
- W2568763370 cites W2149184368 @default.
- W2568763370 cites W2151460035 @default.
- W2568763370 cites W2161605421 @default.
- W2568763370 cites W2163756713 @default.
- W2568763370 cites W2179425537 @default.
- W2568763370 cites W2275524693 @default.
- W2568763370 cites W2282846220 @default.
- W2568763370 cites W2334528087 @default.
- W2568763370 cites W2471933976 @default.
- W2568763370 cites W2474548355 @default.
- W2568763370 cites W4211156111 @default.
- W2568763370 cites W4246043393 @default.
- W2568763370 cites W955725593 @default.
- W2568763370 doi "https://doi.org/10.1021/acs.analchem.6b02875" @default.
- W2568763370 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5299547" @default.
- W2568763370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28208298" @default.
- W2568763370 hasPublicationYear "2017" @default.
- W2568763370 type Work @default.
- W2568763370 sameAs 2568763370 @default.
- W2568763370 citedByCount "45" @default.
- W2568763370 countsByYear W25687633702017 @default.
- W2568763370 countsByYear W25687633702018 @default.
- W2568763370 countsByYear W25687633702019 @default.
- W2568763370 countsByYear W25687633702020 @default.
- W2568763370 countsByYear W25687633702021 @default.
- W2568763370 countsByYear W25687633702022 @default.
- W2568763370 countsByYear W25687633702023 @default.
- W2568763370 crossrefType "journal-article" @default.
- W2568763370 hasAuthorship W2568763370A5049119135 @default.
- W2568763370 hasAuthorship W2568763370A5065901458 @default.