Matches in SemOpenAlex for { <https://semopenalex.org/work/W2569763913> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2569763913 endingPage "76" @default.
- W2569763913 startingPage "68" @default.
- W2569763913 abstract "Efficient drug delivery systems are currently one of the greatest challenges in pharmacokinetics, and the transposition of the gap between in vitro candidate molecule and in vivo test drug is, sometimes, poles apart. In this sense, the cell-penetrating peptides (CPP) may be the bridge uniting these worlds. Here, we describe a technique to rapidly identify unlabeled CPPs after incubation with liposomes, based on commercial desalting (size exclusion) columns and liquid chromatography-MS/MS, for peptide de novo sequencing. Using this approach, we found it possible to identify one new CPP - interestingly, a classical bradykinin-potentiating peptide - in the peptide-rich low molecular mass fraction of the Bothrops jararaca venom, which was also able to penetrate live cell membranes, as confirmed by classical approaches employing fluorescence-labeled analogues of this CPP. Moreover, both the labeled and unlabeled CPPs caused no metabolic, cell-cycle or morphologic alterations, proving to be unmistakably cargo deliverers and not drugs themselves. In sum, we have developed and validated a method for screening label-free peptides for CPP activity, regardless of their biological origin, which could lead to the identification of new and more efficient drug delivery systems. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd." @default.
- W2569763913 created "2017-01-13" @default.
- W2569763913 creator A5020024431 @default.
- W2569763913 creator A5032120983 @default.
- W2569763913 creator A5034923468 @default.
- W2569763913 creator A5042183935 @default.
- W2569763913 creator A5066237562 @default.
- W2569763913 creator A5089160511 @default.
- W2569763913 date "2017-01-01" @default.
- W2569763913 modified "2023-09-24" @default.
- W2569763913 title "An unexpected cell-penetrating peptide from<i>Bothrops jararaca</i>venom identified through a novel size exclusion chromatography screening" @default.
- W2569763913 cites W1670114899 @default.
- W2569763913 cites W1965077313 @default.
- W2569763913 cites W1972729210 @default.
- W2569763913 cites W1990733397 @default.
- W2569763913 cites W1995709619 @default.
- W2569763913 cites W2029346749 @default.
- W2569763913 cites W2045022209 @default.
- W2569763913 cites W2050428252 @default.
- W2569763913 cites W2066434469 @default.
- W2569763913 cites W2069414163 @default.
- W2569763913 cites W2072581984 @default.
- W2569763913 cites W2081786688 @default.
- W2569763913 cites W2110258116 @default.
- W2569763913 cites W2135986253 @default.
- W2569763913 cites W2156818122 @default.
- W2569763913 cites W2161647621 @default.
- W2569763913 cites W2318393308 @default.
- W2569763913 cites W2320357709 @default.
- W2569763913 cites W4245676270 @default.
- W2569763913 doi "https://doi.org/10.1002/psc.2965" @default.
- W2569763913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28054409" @default.
- W2569763913 hasPublicationYear "2017" @default.
- W2569763913 type Work @default.
- W2569763913 sameAs 2569763913 @default.
- W2569763913 citedByCount "11" @default.
- W2569763913 countsByYear W25697639132018 @default.
- W2569763913 countsByYear W25697639132019 @default.
- W2569763913 countsByYear W25697639132020 @default.
- W2569763913 countsByYear W25697639132021 @default.
- W2569763913 countsByYear W25697639132022 @default.
- W2569763913 countsByYear W25697639132023 @default.
- W2569763913 crossrefType "journal-article" @default.
- W2569763913 hasAuthorship W2569763913A5020024431 @default.
- W2569763913 hasAuthorship W2569763913A5032120983 @default.
- W2569763913 hasAuthorship W2569763913A5034923468 @default.
- W2569763913 hasAuthorship W2569763913A5042183935 @default.
- W2569763913 hasAuthorship W2569763913A5066237562 @default.
- W2569763913 hasAuthorship W2569763913A5089160511 @default.
- W2569763913 hasConcept C150903083 @default.
- W2569763913 hasConcept C181199279 @default.
- W2569763913 hasConcept C185592680 @default.
- W2569763913 hasConcept C193363816 @default.
- W2569763913 hasConcept C202751555 @default.
- W2569763913 hasConcept C207001950 @default.
- W2569763913 hasConcept C2776725428 @default.
- W2569763913 hasConcept C2776929087 @default.
- W2569763913 hasConcept C2779281246 @default.
- W2569763913 hasConcept C2779448229 @default.
- W2569763913 hasConcept C2909919539 @default.
- W2569763913 hasConcept C55493867 @default.
- W2569763913 hasConcept C86803240 @default.
- W2569763913 hasConceptScore W2569763913C150903083 @default.
- W2569763913 hasConceptScore W2569763913C181199279 @default.
- W2569763913 hasConceptScore W2569763913C185592680 @default.
- W2569763913 hasConceptScore W2569763913C193363816 @default.
- W2569763913 hasConceptScore W2569763913C202751555 @default.
- W2569763913 hasConceptScore W2569763913C207001950 @default.
- W2569763913 hasConceptScore W2569763913C2776725428 @default.
- W2569763913 hasConceptScore W2569763913C2776929087 @default.
- W2569763913 hasConceptScore W2569763913C2779281246 @default.
- W2569763913 hasConceptScore W2569763913C2779448229 @default.
- W2569763913 hasConceptScore W2569763913C2909919539 @default.
- W2569763913 hasConceptScore W2569763913C55493867 @default.
- W2569763913 hasConceptScore W2569763913C86803240 @default.
- W2569763913 hasFunder F4320322025 @default.
- W2569763913 hasIssue "1" @default.
- W2569763913 hasLocation W25697639131 @default.
- W2569763913 hasLocation W25697639132 @default.
- W2569763913 hasOpenAccess W2569763913 @default.
- W2569763913 hasPrimaryLocation W25697639131 @default.
- W2569763913 hasRelatedWork W1999289052 @default.
- W2569763913 hasRelatedWork W2045673607 @default.
- W2569763913 hasRelatedWork W2061852709 @default.
- W2569763913 hasRelatedWork W2080067651 @default.
- W2569763913 hasRelatedWork W2081003561 @default.
- W2569763913 hasRelatedWork W2259206372 @default.
- W2569763913 hasRelatedWork W2377145913 @default.
- W2569763913 hasRelatedWork W2383939255 @default.
- W2569763913 hasRelatedWork W4296260567 @default.
- W2569763913 hasRelatedWork W178420359 @default.
- W2569763913 hasVolume "23" @default.
- W2569763913 isParatext "false" @default.
- W2569763913 isRetracted "false" @default.
- W2569763913 magId "2569763913" @default.
- W2569763913 workType "article" @default.