Matches in SemOpenAlex for { <https://semopenalex.org/work/W2569793790> ?p ?o ?g. }
- W2569793790 endingPage "1341" @default.
- W2569793790 startingPage "1332" @default.
- W2569793790 abstract "Intravitreal recombinant tissue plasminogen activator (rtPA) is used off-label for the surgical management of submacular hemorrhage, a severe complication of neovascular age-related macular degeneration. rtPA is approved for coronary and cerebral thrombolysis. However, in ischemic stroke rtPA is known to increase excitotoxic neural cell death by interacting with the N-methyl-D-aspartate (NMDA) receptor. We therefore investigated the retinal toxicity of rtPA in healthy rats and in a model of NMDA-induced retinal excitotoxicity.First, rtPA at three different doses (2.16 µg/5 µl, 0.54 µg/5 µl, and 0.27 µg/5 µl) or vehicle (NaCl 0.9%) was injected intravitreally in healthy rat eyes. Electroretinograms (ERGs) were performed at 24 h or 7 days. Annexin V-fluorescein isothiocyanate (FITC)-labeled apoptotic retinal ganglion cells (RGCs) were counted on flatmounted retinas at 24 h or 7 days. Next, NMDA + vehicle or NMDA + rtPA (0.27 µg/5 µl) was injected intravitreally to generate excitotoxic conditions. Apoptotic annexin V-FITC-labeled RGCs and surviving Brn3a-labeled RGCs were quantified on flatmounted retinas and radial sections, 18 h after treatment.In healthy rat eyes, the number of apoptotic RGCs was statistically significantly increased 24 h after the administration of rtPA at the highest dose (2.16 µg/5 µl; p = 0.0250) but not at the lower doses of 0.54 and 0.27 µg/5 µl (p = 0.36 and p = 0.20), compared to vehicle. At day 7, there was no difference in the apoptotic RGC count between the rtPA- and vehicle-injected eyes (p = 0.70, p = 0.52, p = 0.11). ERG amplitudes and implicit times were not modified at 24 h or 7 days after injection of any tested rtPA doses, compared to the baseline. Intravitreal administration of NMDA induced RGC death, but under these excitotoxic conditions, coadministration of rtPA did not increase the number of dead RGCs (p = 0.70). Similarly, the number of surviving RGCs on the flatmounted retinas and retinal sections did not differ between the eyes injected with NMDA + vehicle and NMDA + rtPA (p = 0.59 and p = 0.67).At low clinical equivalent doses corresponding to 25 µg/0.1 ml in humans, intravitreal rtPA is not toxic for healthy rat retinas and does not enhance NMDA-induced excitotoxicity. Vitreal equivalent doses ≥200 µg/0.1 ml should be avoided in patients, due to potential RGC toxicity." @default.
- W2569793790 created "2017-01-13" @default.
- W2569793790 creator A5001469550 @default.
- W2569793790 creator A5001527471 @default.
- W2569793790 creator A5004749164 @default.
- W2569793790 creator A5006724690 @default.
- W2569793790 creator A5007791493 @default.
- W2569793790 creator A5013948563 @default.
- W2569793790 creator A5039480585 @default.
- W2569793790 creator A5079780744 @default.
- W2569793790 creator A5085871115 @default.
- W2569793790 creator A5090983053 @default.
- W2569793790 date "2016-01-01" @default.
- W2569793790 modified "2023-10-18" @default.
- W2569793790 title "Retinal safety of intravitreal rtPA in healthy rats and under excitotoxic conditions." @default.
- W2569793790 cites W1494500191 @default.
- W2569793790 cites W16060373 @default.
- W2569793790 cites W1961340888 @default.
- W2569793790 cites W1967894882 @default.
- W2569793790 cites W1969982263 @default.
- W2569793790 cites W1971468895 @default.
- W2569793790 cites W1972154748 @default.
- W2569793790 cites W1975089240 @default.
- W2569793790 cites W1975257064 @default.
- W2569793790 cites W1978190853 @default.
- W2569793790 cites W1980926873 @default.
- W2569793790 cites W1981910175 @default.
- W2569793790 cites W1987813682 @default.
- W2569793790 cites W1993974622 @default.
- W2569793790 cites W2004695476 @default.
- W2569793790 cites W2013662783 @default.
- W2569793790 cites W2018732055 @default.
- W2569793790 cites W2020486851 @default.
- W2569793790 cites W2021390723 @default.
- W2569793790 cites W2026951106 @default.
- W2569793790 cites W2029650842 @default.
- W2569793790 cites W2029693875 @default.
- W2569793790 cites W2030131304 @default.
- W2569793790 cites W2032358631 @default.
- W2569793790 cites W2037064786 @default.
- W2569793790 cites W2044762414 @default.
- W2569793790 cites W2046885395 @default.
- W2569793790 cites W2052983129 @default.
- W2569793790 cites W2053577892 @default.
- W2569793790 cites W2055242016 @default.
- W2569793790 cites W2055811084 @default.
- W2569793790 cites W2060651294 @default.
- W2569793790 cites W2077306263 @default.
- W2569793790 cites W2081776993 @default.
- W2569793790 cites W2083256895 @default.
- W2569793790 cites W2083477016 @default.
- W2569793790 cites W2085107422 @default.
- W2569793790 cites W2086970243 @default.
- W2569793790 cites W2088877548 @default.
- W2569793790 cites W2113198316 @default.
- W2569793790 cites W2121209701 @default.
- W2569793790 cites W2138615044 @default.
- W2569793790 cites W2147994959 @default.
- W2569793790 cites W2162239484 @default.
- W2569793790 cites W2860150729 @default.
- W2569793790 cites W3023654776 @default.
- W2569793790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5108461" @default.
- W2569793790 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27881907" @default.
- W2569793790 hasPublicationYear "2016" @default.
- W2569793790 type Work @default.
- W2569793790 sameAs 2569793790 @default.
- W2569793790 citedByCount "1" @default.
- W2569793790 countsByYear W25697937902020 @default.
- W2569793790 crossrefType "journal-article" @default.
- W2569793790 hasAuthorship W2569793790A5001469550 @default.
- W2569793790 hasAuthorship W2569793790A5001527471 @default.
- W2569793790 hasAuthorship W2569793790A5004749164 @default.
- W2569793790 hasAuthorship W2569793790A5006724690 @default.
- W2569793790 hasAuthorship W2569793790A5007791493 @default.
- W2569793790 hasAuthorship W2569793790A5013948563 @default.
- W2569793790 hasAuthorship W2569793790A5039480585 @default.
- W2569793790 hasAuthorship W2569793790A5079780744 @default.
- W2569793790 hasAuthorship W2569793790A5085871115 @default.
- W2569793790 hasAuthorship W2569793790A5090983053 @default.
- W2569793790 hasConcept C118487528 @default.
- W2569793790 hasConcept C126322002 @default.
- W2569793790 hasConcept C169760540 @default.
- W2569793790 hasConcept C170493617 @default.
- W2569793790 hasConcept C2776572282 @default.
- W2569793790 hasConcept C2777093970 @default.
- W2569793790 hasConcept C2780827179 @default.
- W2569793790 hasConcept C2781012912 @default.
- W2569793790 hasConcept C42219234 @default.
- W2569793790 hasConcept C61174792 @default.
- W2569793790 hasConcept C67018056 @default.
- W2569793790 hasConcept C71924100 @default.
- W2569793790 hasConcept C86803240 @default.
- W2569793790 hasConcept C98274493 @default.
- W2569793790 hasConceptScore W2569793790C118487528 @default.
- W2569793790 hasConceptScore W2569793790C126322002 @default.
- W2569793790 hasConceptScore W2569793790C169760540 @default.
- W2569793790 hasConceptScore W2569793790C170493617 @default.
- W2569793790 hasConceptScore W2569793790C2776572282 @default.