FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2569884865> ?p ?o ?g. }

• W2569884865 endingPage "83" @default.
• W2569884865 startingPage "75" @default.
• W2569884865 abstract "O-methylation of the side chains of glutamic acid (E) and aspartic acid (D) residues is generally observed modification when an acidified methanol/water (MeOH/dH2O) mixture is used as a solvent system during sample preparation for proteomic research. This chemical modification may result misidentification with endogenous protein methylation; therefore, a special care should be taken during sample handling prior to mass spectrometric analysis. In the current study, we systematically examined the extent of E/D methylation and C-terminus carboxyl group of synthetic model peptides in terms of different incubation temperatures, storage times, and added acid types as well as its percentages. To monitor these effects, C-terminus amidated and free acid forms of synthetic model peptides comprised of E or D residue(s) have been analyzed by electrospray ionization-mass spectrometry (ESI-MS). Additionally, LC–MS/MS experiments were performed to confirm the formation of methylated peptide product. The results showed that the rate of methylation was increased as the temperature increases along with prolong incubation times. Moreover, the extent of methylation was remarkably high when formic acid (FA) used as a protonation agent instead of acetic acid (AA). In addition, it was found that the degree of methylation was significantly decreased by lowering acid percentages in ESI solution. More than one acidic residue containing model peptides have been also used to explore the extent of multiple methylation reaction. Lastly, the ethanol (EtOH) and isopropanol (iPrOH) have been substituted separately with MeOH in sample preparation step to investigate the extent of esterification reaction under the same experimental conditions. However, in the positive perspective of view, this method can be used as a simple, rapid and cheap method for methylation of acidic residues under normal laboratory conditions." @default.
• W2569884865 created "2017-01-13" @default.
• W2569884865 creator A5023403646 @default.
• W2569884865 creator A5064308662 @default.
• W2569884865 creator A5069102745 @default.
• W2569884865 date "2017-03-01" @default.
• W2569884865 modified "2023-09-26" @default.
• W2569884865 title "Observation of the side chain O -methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometry" @default.
• W2569884865 cites W1967790997 @default.
• W2569884865 cites W1977761119 @default.
• W2569884865 cites W1982015656 @default.
• W2569884865 cites W1989648459 @default.
• W2569884865 cites W2002698073 @default.
• W2569884865 cites W2013813317 @default.
• W2569884865 cites W2014214992 @default.
• W2569884865 cites W2022505591 @default.
• W2569884865 cites W2028161249 @default.
• W2569884865 cites W2035700643 @default.
• W2569884865 cites W2047467738 @default.
• W2569884865 cites W2063717476 @default.
• W2569884865 cites W2073292063 @default.
• W2569884865 cites W2076142854 @default.
• W2569884865 cites W2078239448 @default.
• W2569884865 cites W2079056345 @default.
• W2569884865 cites W2086671638 @default.
• W2569884865 cites W2091921454 @default.
• W2569884865 cites W2092809155 @default.
• W2569884865 cites W2093509030 @default.
• W2569884865 cites W2117399884 @default.
• W2569884865 cites W2127456719 @default.
• W2569884865 cites W2141707814 @default.
• W2569884865 cites W2157836791 @default.
• W2569884865 cites W2166805033 @default.
• W2569884865 cites W2473612153 @default.
• W2569884865 doi "https://doi.org/10.1016/j.jchromb.2016.12.043" @default.
• W2569884865 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28063777" @default.
• W2569884865 hasPublicationYear "2017" @default.
• W2569884865 type Work @default.
• W2569884865 sameAs 2569884865 @default.
• W2569884865 citedByCount "1" @default.
• W2569884865 countsByYear W25698848652023 @default.
• W2569884865 crossrefType "journal-article" @default.
• W2569884865 hasAuthorship W2569884865A5023403646 @default.
• W2569884865 hasAuthorship W2569884865A5064308662 @default.
• W2569884865 hasAuthorship W2569884865A5069102745 @default.
• W2569884865 hasConcept C104317684 @default.
• W2569884865 hasConcept C145148216 @default.
• W2569884865 hasConcept C162356407 @default.
• W2569884865 hasConcept C178790620 @default.
• W2569884865 hasConcept C185592680 @default.
• W2569884865 hasConcept C194451485 @default.
• W2569884865 hasConcept C2777379063 @default.
• W2569884865 hasConcept C2778740566 @default.
• W2569884865 hasConcept C2781208047 @default.
• W2569884865 hasConcept C2781338088 @default.
• W2569884865 hasConcept C30095370 @default.
• W2569884865 hasConcept C33288867 @default.
• W2569884865 hasConcept C40684141 @default.
• W2569884865 hasConcept C43617362 @default.
• W2569884865 hasConcept C515207424 @default.
• W2569884865 hasConcept C55493867 @default.
• W2569884865 hasConceptScore W2569884865C104317684 @default.
• W2569884865 hasConceptScore W2569884865C145148216 @default.
• W2569884865 hasConceptScore W2569884865C162356407 @default.
• W2569884865 hasConceptScore W2569884865C178790620 @default.
• W2569884865 hasConceptScore W2569884865C185592680 @default.
• W2569884865 hasConceptScore W2569884865C194451485 @default.
• W2569884865 hasConceptScore W2569884865C2777379063 @default.
• W2569884865 hasConceptScore W2569884865C2778740566 @default.
• W2569884865 hasConceptScore W2569884865C2781208047 @default.
• W2569884865 hasConceptScore W2569884865C2781338088 @default.
• W2569884865 hasConceptScore W2569884865C30095370 @default.
• W2569884865 hasConceptScore W2569884865C33288867 @default.
• W2569884865 hasConceptScore W2569884865C40684141 @default.
• W2569884865 hasConceptScore W2569884865C43617362 @default.
• W2569884865 hasConceptScore W2569884865C515207424 @default.
• W2569884865 hasConceptScore W2569884865C55493867 @default.
• W2569884865 hasLocation W25698848651 @default.
• W2569884865 hasLocation W25698848652 @default.
• W2569884865 hasOpenAccess W2569884865 @default.
• W2569884865 hasPrimaryLocation W25698848651 @default.
• W2569884865 hasRelatedWork W1277187992 @default.
• W2569884865 hasRelatedWork W1562712502 @default.
• W2569884865 hasRelatedWork W1971295290 @default.
• W2569884865 hasRelatedWork W1984116082 @default.
• W2569884865 hasRelatedWork W2000794674 @default.
• W2569884865 hasRelatedWork W2050011283 @default.
• W2569884865 hasRelatedWork W2052715446 @default.
• W2569884865 hasRelatedWork W2354088362 @default.
• W2569884865 hasRelatedWork W2569884865 @default.
• W2569884865 hasRelatedWork W274350598 @default.
• W2569884865 hasVolume "1047" @default.
• W2569884865 isParatext "false" @default.
• W2569884865 isRetracted "false" @default.
• W2569884865 magId "2569884865" @default.
• W2569884865 workType "article" @default.