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- W257017043 abstract "Immunology is an alternative assay for phytochrome. The assay used to isolate, purify, and characterize phytochrome is derived from the unique, photoreversible spectral properties of this chromoprotein. However, it has long been apparent that this spectral assay suffers from a number of limitations. The spectral assay is not suitable for studies of phytochrome within single, defined cells. Additional means of assay for phytochrome are clearly desirable. While available immunochemical assays, such as the spectral assay, provide no direct information concerning biological activity of the phytochrome being measured, the immunochemical assays, unlike the spectral assay, are neither dependent on the presence of the chromophore nor on its extinction coefficient and, thus, may be used as a direct measure of the amount of phytochrome protein present. The chapter indicates that immunochemical assays do not replace the spectral assay but rather supplement it and, thus, allow one to answer questions that cannot be answered by using only the spectral assay. An example of the potential of the immunological assay system is demonstrated by calculations that indicate that picogram levels of phytochrome should be detectable by means of a radioimmunoassay." @default.
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- W257017043 date "1976-01-01" @default.
- W257017043 modified "2023-09-27" @default.
- W257017043 title "IMMUNOLOGICAL VISUALISATION OF PHYTOCHROME" @default.
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- W257017043 doi "https://doi.org/10.1016/b978-0-408-70719-0.50012-7" @default.
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