FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2570253006> ?p ?o ?g. }

• W2570253006 endingPage "546" @default.
• W2570253006 startingPage "540" @default.
• W2570253006 abstract "Despite advances in microbial protein expression systems, low production of proteins remains a great concern for some genes. Here we report that the insertion of a short peptide tag, consisting of Ser-Lys-Ile-Lys (SKIK), adjacent to the start codon of genes encoding difficult-to-express proteins can increase protein expression in Escherichia coli and Saccharomyces cerevisiae. Protein expression levels of a mouse monoclonal antibody (mAb), rabbit mAbs obtained from clonal B cells, and an artificially designed peptide were significantly increased simply by the addition of the SKIK tag in E. coli systems. In particular, a ∼30-fold increase in protein production was observed for the mouse mAb, and the artificially designed peptide band became detectable in sodium dodecyl sulfate-poly acrylamide gel electrophoresis after coomassie brilliant blue staining or western blotting on adding the SKIK tag. The tag also increased the expression of tagged proteins in S. cerevisiae and an E. coli cell-free protein synthesis system. Although the mechanism of high protein expression on addition of the tag is unclear, our findings offer great benefits to biotechnology research and industry." @default.
• W2570253006 created "2017-01-13" @default.
• W2570253006 creator A5070778902 @default.
• W2570253006 creator A5078554949 @default.
• W2570253006 creator A5088658960 @default.
• W2570253006 date "2017-05-01" @default.
• W2570253006 modified "2023-10-05" @default.
• W2570253006 title "N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae" @default.
• W2570253006 cites W12220879 @default.
• W2570253006 cites W1532816416 @default.
• W2570253006 cites W1587481053 @default.
• W2570253006 cites W1884779989 @default.
• W2570253006 cites W1967245701 @default.
• W2570253006 cites W1971453673 @default.
• W2570253006 cites W1973448897 @default.
• W2570253006 cites W1981078760 @default.
• W2570253006 cites W1988583600 @default.
• W2570253006 cites W2010546547 @default.
• W2570253006 cites W2011501000 @default.
• W2570253006 cites W2025569664 @default.
• W2570253006 cites W2035475950 @default.
• W2570253006 cites W2039054922 @default.
• W2570253006 cites W2046872786 @default.
• W2570253006 cites W2051242197 @default.
• W2570253006 cites W2065366746 @default.
• W2570253006 cites W2068446924 @default.
• W2570253006 cites W2073575016 @default.
• W2570253006 cites W2080434522 @default.
• W2570253006 cites W2087807518 @default.
• W2570253006 cites W2097152121 @default.
• W2570253006 cites W2101918141 @default.
• W2570253006 cites W2120368500 @default.
• W2570253006 cites W2122500226 @default.
• W2570253006 cites W2127612970 @default.
• W2570253006 cites W2134887033 @default.
• W2570253006 cites W2135303614 @default.
• W2570253006 cites W2138820253 @default.
• W2570253006 cites W2147837708 @default.
• W2570253006 cites W2157913328 @default.
• W2570253006 cites W2259283565 @default.
• W2570253006 cites W2305060114 @default.
• W2570253006 doi "https://doi.org/10.1016/j.jbiosc.2016.12.004" @default.
• W2570253006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28052816" @default.
• W2570253006 hasPublicationYear "2017" @default.
• W2570253006 type Work @default.
• W2570253006 sameAs 2570253006 @default.
• W2570253006 citedByCount "30" @default.
• W2570253006 countsByYear W25702530062017 @default.
• W2570253006 countsByYear W25702530062018 @default.
• W2570253006 countsByYear W25702530062019 @default.
• W2570253006 countsByYear W25702530062020 @default.
• W2570253006 countsByYear W25702530062021 @default.
• W2570253006 countsByYear W25702530062022 @default.
• W2570253006 countsByYear W25702530062023 @default.
• W2570253006 crossrefType "journal-article" @default.
• W2570253006 hasAuthorship W2570253006A5070778902 @default.
• W2570253006 hasAuthorship W2570253006A5078554949 @default.
• W2570253006 hasAuthorship W2570253006A5088658960 @default.
• W2570253006 hasConcept C104317684 @default.
• W2570253006 hasConcept C116084860 @default.
• W2570253006 hasConcept C123894998 @default.
• W2570253006 hasConcept C150194340 @default.
• W2570253006 hasConcept C153911025 @default.
• W2570253006 hasConcept C156904656 @default.
• W2570253006 hasConcept C2777576037 @default.
• W2570253006 hasConcept C2779281246 @default.
• W2570253006 hasConcept C2780761556 @default.
• W2570253006 hasConcept C40767141 @default.
• W2570253006 hasConcept C54355233 @default.
• W2570253006 hasConcept C547475151 @default.
• W2570253006 hasConcept C55493867 @default.
• W2570253006 hasConcept C74864618 @default.
• W2570253006 hasConcept C86803240 @default.
• W2570253006 hasConceptScore W2570253006C104317684 @default.
• W2570253006 hasConceptScore W2570253006C116084860 @default.
• W2570253006 hasConceptScore W2570253006C123894998 @default.
• W2570253006 hasConceptScore W2570253006C150194340 @default.
• W2570253006 hasConceptScore W2570253006C153911025 @default.
• W2570253006 hasConceptScore W2570253006C156904656 @default.
• W2570253006 hasConceptScore W2570253006C2777576037 @default.
• W2570253006 hasConceptScore W2570253006C2779281246 @default.
• W2570253006 hasConceptScore W2570253006C2780761556 @default.
• W2570253006 hasConceptScore W2570253006C40767141 @default.
• W2570253006 hasConceptScore W2570253006C54355233 @default.
• W2570253006 hasConceptScore W2570253006C547475151 @default.
• W2570253006 hasConceptScore W2570253006C55493867 @default.
• W2570253006 hasConceptScore W2570253006C74864618 @default.
• W2570253006 hasConceptScore W2570253006C86803240 @default.
• W2570253006 hasFunder F4320324712 @default.
• W2570253006 hasIssue "5" @default.
• W2570253006 hasLocation W25702530061 @default.
• W2570253006 hasLocation W25702530062 @default.
• W2570253006 hasOpenAccess W2570253006 @default.
• W2570253006 hasPrimaryLocation W25702530061 @default.
• W2570253006 hasRelatedWork W1546752876 @default.
• W2570253006 hasRelatedWork W2009966535 @default.
• W2570253006 hasRelatedWork W2080910126 @default.
• W2570253006 hasRelatedWork W2088063203 @default.

• next