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- W2570303721 abstract "Vaccines remain one of the most effective ways to prevent infectious diseases globally. Accurate measurement of thiomersal content is an important parameter to ensure safety of vaccines though these vaccines are less in use. In the present study a simple, accurate and highly effective spectrophotometric improved method has been developed for determination of thiomersal in Hib-TT conjugate vaccine by using 0.01% and 0.005% concentration of dithizone for making stock and comparing these with 0.001% dithizone concentration mentioned in Indian Pharmacopoeia (2014). All the concentrations were evaluated at three different wavelength i.e. 538nm, 540 nm and 542nm. When all the three concentrations compared, the dithizone concentration 0.01 % has been found to be more accurate, linear and giving reproducible results. Among the three wavelengths, the best result has been obtained at wavelength 538nm. The improved method is accurate, specific, and easy to carry out, consumes little time and requires a few microliters of sample and therefore, improved method is recommended than the present pharmacopoeial method which underestimate the concentration of thiomersal and fails the otherwise passed Hib-TT conjugate vaccine." @default.
- W2570303721 created "2017-01-13" @default.
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- W2570303721 date "2016-12-30" @default.
- W2570303721 modified "2023-09-24" @default.
- W2570303721 title "Improved Spectrophotometric Pharmacopoeial Method for accurate Quantitation of Thiomersal content in Haemophilus influenzae type b (Hib)-TT Conjugate Vaccine" @default.
- W2570303721 doi "https://doi.org/10.7439/ijbar.v7i12.3689" @default.
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