Matches in SemOpenAlex for { <https://semopenalex.org/work/W2570355308> ?p ?o ?g. }
- W2570355308 endingPage "616" @default.
- W2570355308 startingPage "608" @default.
- W2570355308 abstract "Extensive research has been performed to determine the effect of freezing protocol and cryopreservation agents on the viability of adipose tissue-derived stromal/stem cells (ASCs) as well as other cells. Unfortunately, the conclusion one may draw after decades of research utilizing fundamentally similar cryopreservation techniques is that a barrier exists, which precludes full recovery. We hypothesize that agents capable of inducing a subset of heat shock proteins (HSPs) and chaperones will reduce the intrinsic barriers to the post-thaw recovery of ASCs. ASCs were exposed to 43°C for 1 h to upregulate HSPs, and the temporal HSP expression profile postheat shock was determined by performing quantitative polymerase chain reaction (PCR) and western blotting assays. The expression levels of HSP70 and HSP32 were found to be maximum at 3 h after the heat shock, whereas HSP90 and HSP27 remain unchanged. The heat shocked ASCs cryopreserved during maximal HSPs expression exhibited increased post-thaw viability than the nonheat shocked samples. Histochemical staining and quantitative reverse transcription-PCR indicated that the ASC differentiation potential was retained. Thus, suggesting that the upregulation of HSPs before a freezing insult is beneficial to ASCs and a potential alternative to the use of harmful cryoprotective agents." @default.
- W2570355308 created "2017-01-13" @default.
- W2570355308 creator A5008816521 @default.
- W2570355308 creator A5019333768 @default.
- W2570355308 creator A5026038537 @default.
- W2570355308 creator A5071653812 @default.
- W2570355308 date "2017-04-15" @default.
- W2570355308 modified "2023-09-26" @default.
- W2570355308 title "Inducing Heat Shock Proteins Enhances the Stemness of Frozen–Thawed Adipose Tissue-Derived Stem Cells" @default.
- W2570355308 cites W1519030095 @default.
- W2570355308 cites W1549796567 @default.
- W2570355308 cites W1588064010 @default.
- W2570355308 cites W1865247695 @default.
- W2570355308 cites W1971357668 @default.
- W2570355308 cites W1982457774 @default.
- W2570355308 cites W1983905708 @default.
- W2570355308 cites W1984098071 @default.
- W2570355308 cites W1985619283 @default.
- W2570355308 cites W1992595819 @default.
- W2570355308 cites W1995032020 @default.
- W2570355308 cites W1995315566 @default.
- W2570355308 cites W2013228367 @default.
- W2570355308 cites W2023800754 @default.
- W2570355308 cites W2027138640 @default.
- W2570355308 cites W2029190023 @default.
- W2570355308 cites W2031046488 @default.
- W2570355308 cites W2036491313 @default.
- W2570355308 cites W2046639009 @default.
- W2570355308 cites W2056905507 @default.
- W2570355308 cites W2062782211 @default.
- W2570355308 cites W2072925339 @default.
- W2570355308 cites W2074332495 @default.
- W2570355308 cites W2075026527 @default.
- W2570355308 cites W2100155649 @default.
- W2570355308 cites W2107277218 @default.
- W2570355308 cites W2107503723 @default.
- W2570355308 cites W2109606739 @default.
- W2570355308 cites W2109936231 @default.
- W2570355308 cites W2115062578 @default.
- W2570355308 cites W2116259052 @default.
- W2570355308 cites W2116902247 @default.
- W2570355308 cites W2117473217 @default.
- W2570355308 cites W2119874123 @default.
- W2570355308 cites W2128586512 @default.
- W2570355308 cites W2136751440 @default.
- W2570355308 cites W2143609278 @default.
- W2570355308 cites W2148573587 @default.
- W2570355308 cites W2161959128 @default.
- W2570355308 cites W2162749230 @default.
- W2570355308 cites W2172299613 @default.
- W2570355308 cites W2179715594 @default.
- W2570355308 cites W2313037473 @default.
- W2570355308 cites W2407960121 @default.
- W2570355308 cites W2426457886 @default.
- W2570355308 cites W2554672040 @default.
- W2570355308 cites W67858098 @default.
- W2570355308 cites W1964184380 @default.
- W2570355308 doi "https://doi.org/10.1089/scd.2016.0289" @default.
- W2570355308 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5393415" @default.
- W2570355308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28052723" @default.
- W2570355308 hasPublicationYear "2017" @default.
- W2570355308 type Work @default.
- W2570355308 sameAs 2570355308 @default.
- W2570355308 citedByCount "23" @default.
- W2570355308 countsByYear W25703553082018 @default.
- W2570355308 countsByYear W25703553082019 @default.
- W2570355308 countsByYear W25703553082020 @default.
- W2570355308 countsByYear W25703553082021 @default.
- W2570355308 countsByYear W25703553082022 @default.
- W2570355308 crossrefType "journal-article" @default.
- W2570355308 hasAuthorship W2570355308A5008816521 @default.
- W2570355308 hasAuthorship W2570355308A5019333768 @default.
- W2570355308 hasAuthorship W2570355308A5026038537 @default.
- W2570355308 hasAuthorship W2570355308A5071653812 @default.
- W2570355308 hasBestOaLocation W25703553082 @default.
- W2570355308 hasConcept C104317684 @default.
- W2570355308 hasConcept C127561419 @default.
- W2570355308 hasConcept C137984847 @default.
- W2570355308 hasConcept C1491633281 @default.
- W2570355308 hasConcept C153911025 @default.
- W2570355308 hasConcept C16685009 @default.
- W2570355308 hasConcept C16930146 @default.
- W2570355308 hasConcept C171089720 @default.
- W2570355308 hasConcept C179933525 @default.
- W2570355308 hasConcept C196843134 @default.
- W2570355308 hasConcept C205260736 @default.
- W2570355308 hasConcept C2779559962 @default.
- W2570355308 hasConcept C28328180 @default.
- W2570355308 hasConcept C502942594 @default.
- W2570355308 hasConcept C53227056 @default.
- W2570355308 hasConcept C55493867 @default.
- W2570355308 hasConcept C68991219 @default.
- W2570355308 hasConcept C71924100 @default.
- W2570355308 hasConcept C85265743 @default.
- W2570355308 hasConcept C86803240 @default.
- W2570355308 hasConcept C95444343 @default.
- W2570355308 hasConceptScore W2570355308C104317684 @default.
- W2570355308 hasConceptScore W2570355308C127561419 @default.