FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2570397186> ?p ?o ?g. }

• W2570397186 abstract "Abstract Abstract 3979 Adhesion of MM cells to the BM microenvironment can lead to cell adhesion mediated drug resistance (CAM-DR). We have previously shown that treatment with Dexamethasone (a drug commonly used against MM alone or in combination with new therapeutic agents) induces in MM cells increased assembly of CD138, and F-actin containing membrane extensions named podia. Using this 2D co-culture model we found that podia from MM cells from patients or MM cell lines elongate on the surface of BM stromal cells and also interconnect distant MM cells correlating with CAM-DR. Formation of podia was observed after at least 6 days of co-culture when many soluble factors and extracellular matrix proteins may have been secreted and remodelled. We also showed that preventing SDF1-alpha binding to the CXCR4 receptor in MM cells inhibits podia formation. In order to investigate the possible role of ECM proteins and their ligands in podia assembly we have now tested the formation of podia in 3D co-cultures of MM and stromal cells embedded in gels composed of polymerised extracellular matrix proteins found in the BM including collagen I and collagen IV. We now found that MM cells incubated overnight in 3D matrix lattices assembled podia that elongated along fibres of polymerised extracellular matrix that put them in contact with stromal cells or other distant MM cells. We also found that culture of MM cells in 3D matrices in the absence of stromal cells did not result in podia formation. These results suggest that stromal cells promote podia formation in contact with the extracellular matrix. It has been shown by Neri et al1that beta7 integrins are involved in MM cell adhesion to BM cells and extracellular matrix during recruitment to the BM and they are involved in CAM-DR. We found that integrin beta7 KD MM cells failed to form podia to the same extent as the parental cell lines in 2D and 3D cultures. Podia assembled by beta7 KD cells were shorter and highly irregular in shape in comparison to long and straight podia formed by parental MM cells. Using immunostaining, we found that podia in beta7 KD cells lacked internal F-actin as well as vimentin filaments that we identified in podia of parental MM cells. However, total levels of these cytoskeletal proteins remained unchanged as detected by western blot. Dexamethasone-induced increased formation of podia was also dependent on the expression of beta7 integrins by MM cells and the presence of BM stromal cells in 2D and 3D culture conditions. We conclude that podia promote the formation of cellular networks of distant MM cells as well as the contact with BM stromal cells in 3D and this process is involved in CAM-DR against Dexamethasone. Podia assemble in contact with stromal cells and along extracellular matrix cables and require polymerisation of actin and vimentin cytoskeletal filaments downstream of beta7 integrins. Disclosures: No relevant conflicts of interest to declare." @default.
• W2570397186 created "2017-01-13" @default.
• W2570397186 creator A5017679490 @default.
• W2570397186 creator A5020357934 @default.
• W2570397186 creator A5036202306 @default.
• W2570397186 creator A5036647141 @default.
• W2570397186 creator A5058550136 @default.
• W2570397186 creator A5058875013 @default.
• W2570397186 creator A5072918409 @default.
• W2570397186 creator A5090206694 @default.
• W2570397186 date "2012-11-16" @default.
• W2570397186 modified "2023-09-28" @default.
• W2570397186 title "Beta7 Integrins Regulate Podia Formation in Multiple Myeloma (MM) Cells for the Interaction with the Cellular and Non-Cellular Bone Marrow (BM) Stroma" @default.
• W2570397186 doi "https://doi.org/10.1182/blood.v120.21.3979.3979" @default.
• W2570397186 hasPublicationYear "2012" @default.
• W2570397186 type Work @default.
• W2570397186 sameAs 2570397186 @default.
• W2570397186 citedByCount "0" @default.
• W2570397186 crossrefType "journal-article" @default.
• W2570397186 hasAuthorship W2570397186A5017679490 @default.
• W2570397186 hasAuthorship W2570397186A5020357934 @default.
• W2570397186 hasAuthorship W2570397186A5036202306 @default.
• W2570397186 hasAuthorship W2570397186A5036647141 @default.
• W2570397186 hasAuthorship W2570397186A5058550136 @default.
• W2570397186 hasAuthorship W2570397186A5058875013 @default.
• W2570397186 hasAuthorship W2570397186A5072918409 @default.
• W2570397186 hasAuthorship W2570397186A5090206694 @default.
• W2570397186 hasConcept C106487976 @default.
• W2570397186 hasConcept C12554922 @default.
• W2570397186 hasConcept C1491633281 @default.
• W2570397186 hasConcept C153911025 @default.
• W2570397186 hasConcept C16930146 @default.
• W2570397186 hasConcept C178790620 @default.
• W2570397186 hasConcept C185592680 @default.
• W2570397186 hasConcept C189165786 @default.
• W2570397186 hasConcept C195687474 @default.
• W2570397186 hasConcept C28406088 @default.
• W2570397186 hasConcept C43617362 @default.
• W2570397186 hasConcept C502942594 @default.
• W2570397186 hasConcept C54355233 @default.
• W2570397186 hasConcept C55493867 @default.
• W2570397186 hasConcept C81885089 @default.
• W2570397186 hasConcept C84416704 @default.
• W2570397186 hasConcept C85789140 @default.
• W2570397186 hasConcept C86803240 @default.
• W2570397186 hasConcept C95444343 @default.
• W2570397186 hasConceptScore W2570397186C106487976 @default.
• W2570397186 hasConceptScore W2570397186C12554922 @default.
• W2570397186 hasConceptScore W2570397186C1491633281 @default.
• W2570397186 hasConceptScore W2570397186C153911025 @default.
• W2570397186 hasConceptScore W2570397186C16930146 @default.
• W2570397186 hasConceptScore W2570397186C178790620 @default.
• W2570397186 hasConceptScore W2570397186C185592680 @default.
• W2570397186 hasConceptScore W2570397186C189165786 @default.
• W2570397186 hasConceptScore W2570397186C195687474 @default.
• W2570397186 hasConceptScore W2570397186C28406088 @default.
• W2570397186 hasConceptScore W2570397186C43617362 @default.
• W2570397186 hasConceptScore W2570397186C502942594 @default.
• W2570397186 hasConceptScore W2570397186C54355233 @default.
• W2570397186 hasConceptScore W2570397186C55493867 @default.
• W2570397186 hasConceptScore W2570397186C81885089 @default.
• W2570397186 hasConceptScore W2570397186C84416704 @default.
• W2570397186 hasConceptScore W2570397186C85789140 @default.
• W2570397186 hasConceptScore W2570397186C86803240 @default.
• W2570397186 hasConceptScore W2570397186C95444343 @default.
• W2570397186 hasLocation W25703971861 @default.
• W2570397186 hasOpenAccess W2570397186 @default.
• W2570397186 hasPrimaryLocation W25703971861 @default.
• W2570397186 hasRelatedWork W1533562976 @default.
• W2570397186 hasRelatedWork W1536108640 @default.
• W2570397186 hasRelatedWork W1536518696 @default.
• W2570397186 hasRelatedWork W1964217320 @default.
• W2570397186 hasRelatedWork W1982174012 @default.
• W2570397186 hasRelatedWork W1997141270 @default.
• W2570397186 hasRelatedWork W2023667741 @default.
• W2570397186 hasRelatedWork W2024292370 @default.
• W2570397186 hasRelatedWork W2062836063 @default.
• W2570397186 hasRelatedWork W2070068708 @default.
• W2570397186 hasRelatedWork W2074190587 @default.
• W2570397186 hasRelatedWork W2074244127 @default.
• W2570397186 hasRelatedWork W2086446280 @default.
• W2570397186 hasRelatedWork W2089206964 @default.
• W2570397186 hasRelatedWork W2134030378 @default.
• W2570397186 hasRelatedWork W2188194273 @default.
• W2570397186 hasRelatedWork W2221955516 @default.
• W2570397186 hasRelatedWork W2397129617 @default.
• W2570397186 hasRelatedWork W2470882658 @default.
• W2570397186 hasRelatedWork W3155918349 @default.
• W2570397186 isParatext "false" @default.
• W2570397186 isRetracted "false" @default.
• W2570397186 magId "2570397186" @default.
• W2570397186 workType "article" @default.