FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2571382953> ?p ?o ?g. }

• W2571382953 endingPage "e0169403" @default.
• W2571382953 startingPage "e0169403" @default.
• W2571382953 abstract "Scedosporium aurantiacum is an opportunistic filamentous fungus increasingly isolated from the sputum of cystic fibrosis patients, and is especially prevalent in Australia. At the moment, very little is known about the infection mechanism of this fungus. Secreted proteases have been shown to contribute to fungal virulence in several studies with other fungi. Here we have compared the profiles of proteases secreted by a clinical isolate Scedosporium aurantiacum (WM 06.482) and an environmental strain (WM 10.136) grown on a synthetic cystic fibrosis sputum medium supplemented with casein or mucin. Protease activity was assessed using class-specific substrates and inhibitors. Subtilisin-like and trypsin-like serine protease activity was detected in all cultures. The greatest difference in the secretion of proteases between the two strains occurred in mucin-supplemented medium, where the activities of the elastase-like, trypsin-like and aspartic proteases were, overall, 2.5–75 fold higher in the clinical strain compared to the environmental strain. Proteases secreted by the two strains in the mucin-supplemented medium were further analyzed by mass spectrometry. Six homologs of fungal proteases were identified from the clinical strain and five from the environmental strain. Of these, three were common for both strains including a subtilisin peptidase, a putative leucine aminopeptidase and a PA-SaNapH-like protease. Trypsin-like protease was identified by mass spectrometry only in the clinical isolate even though trypsin-like activity was present in all cultures. In contrast, high elastase-like activity was measured in the culture supernatant of the clinical strain but could not be identified by mass spectrometry searching against other fungi in the NCBI database. Future availability of an annotated genome will help finalise identification of the S. aurantiacum proteases." @default.
• W2571382953 created "2017-01-13" @default.
• W2571382953 creator A5005296981 @default.
• W2571382953 creator A5044873766 @default.
• W2571382953 creator A5086363018 @default.
• W2571382953 date "2017-01-06" @default.
• W2571382953 modified "2023-10-18" @default.
• W2571382953 title "Secretion of Proteases by an Opportunistic Fungal Pathogen Scedosporium aurantiacum" @default.
• W2571382953 cites W1487360609 @default.
• W2571382953 cites W1491021866 @default.
• W2571382953 cites W1558513630 @default.
• W2571382953 cites W1609495085 @default.
• W2571382953 cites W1824079566 @default.
• W2571382953 cites W1935518068 @default.
• W2571382953 cites W1967181329 @default.
• W2571382953 cites W1977578761 @default.
• W2571382953 cites W1983052547 @default.
• W2571382953 cites W1987221920 @default.
• W2571382953 cites W1987522014 @default.
• W2571382953 cites W1990513945 @default.
• W2571382953 cites W2002220100 @default.
• W2571382953 cites W2002558747 @default.
• W2571382953 cites W2003817454 @default.
• W2571382953 cites W2003950615 @default.
• W2571382953 cites W2006389393 @default.
• W2571382953 cites W2011117267 @default.
• W2571382953 cites W2011990652 @default.
• W2571382953 cites W2012767918 @default.
• W2571382953 cites W2013703942 @default.
• W2571382953 cites W2016368845 @default.
• W2571382953 cites W2018289835 @default.
• W2571382953 cites W2021015996 @default.
• W2571382953 cites W2027057178 @default.
• W2571382953 cites W2028128554 @default.
• W2571382953 cites W2029790322 @default.
• W2571382953 cites W2030021883 @default.
• W2571382953 cites W2038986380 @default.
• W2571382953 cites W2045658031 @default.
• W2571382953 cites W2052638392 @default.
• W2571382953 cites W2062821234 @default.
• W2571382953 cites W2062831182 @default.
• W2571382953 cites W2063165608 @default.
• W2571382953 cites W2065139915 @default.
• W2571382953 cites W2074614011 @default.
• W2571382953 cites W2085694096 @default.
• W2571382953 cites W2090784961 @default.
• W2571382953 cites W2094321878 @default.
• W2571382953 cites W2095254076 @default.
• W2571382953 cites W2097944090 @default.
• W2571382953 cites W2100837269 @default.
• W2571382953 cites W2104742709 @default.
• W2571382953 cites W2105839986 @default.
• W2571382953 cites W2110439135 @default.
• W2571382953 cites W2116954610 @default.
• W2571382953 cites W2120569572 @default.
• W2571382953 cites W2120924657 @default.
• W2571382953 cites W2122174240 @default.
• W2571382953 cites W2123618159 @default.
• W2571382953 cites W2128635872 @default.
• W2571382953 cites W2132040510 @default.
• W2571382953 cites W2134758330 @default.
• W2571382953 cites W2136334655 @default.
• W2571382953 cites W2137222786 @default.
• W2571382953 cites W2137959581 @default.
• W2571382953 cites W2147745929 @default.
• W2571382953 cites W2147868143 @default.
• W2571382953 cites W2153584505 @default.
• W2571382953 cites W2161633414 @default.
• W2571382953 cites W2161686892 @default.
• W2571382953 cites W2163041926 @default.
• W2571382953 cites W2163717305 @default.
• W2571382953 cites W2164105530 @default.
• W2571382953 cites W2170776775 @default.
• W2571382953 cites W2171258946 @default.
• W2571382953 cites W2263709143 @default.
• W2571382953 cites W2315104107 @default.
• W2571382953 cites W2319903292 @default.
• W2571382953 cites W2324064463 @default.
• W2571382953 cites W2325565338 @default.
• W2571382953 cites W4249295168 @default.
• W2571382953 cites W4250844377 @default.
• W2571382953 cites W4251587918 @default.
• W2571382953 cites W4293247451 @default.
• W2571382953 doi "https://doi.org/10.1371/journal.pone.0169403" @default.
• W2571382953 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5218550" @default.
• W2571382953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28060882" @default.
• W2571382953 hasPublicationYear "2017" @default.
• W2571382953 type Work @default.
• W2571382953 sameAs 2571382953 @default.
• W2571382953 citedByCount "15" @default.
• W2571382953 countsByYear W25713829532017 @default.
• W2571382953 countsByYear W25713829532018 @default.
• W2571382953 countsByYear W25713829532019 @default.
• W2571382953 countsByYear W25713829532020 @default.
• W2571382953 countsByYear W25713829532021 @default.
• W2571382953 countsByYear W25713829532022 @default.
• W2571382953 crossrefType "journal-article" @default.
• W2571382953 hasAuthorship W2571382953A5005296981 @default.

• next