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- W2571403643 abstract "My first foray into porcine molecular genetics commenced about 27 years ago with a small Australian Research Council grant to develop and exploit DNA markers in the pig for genetic mapping. At that stage, the standard procedure for detection of DNA polymorphism involved digestion of genomic DNA with one or more restriction enzymes, electrophoresis, transfer to a membrane, hybridisation with a radioactively labelled probe (using dangerous radioactive phosphorous) and autoradiographic detection on an X-ray film. The detection of these restriction fragment length polymorphisms (RFLPs) was slow, insensitive, expensive and hazardous, but was an important starting point and enabled initial molecular studies of porcine stress syndrome and early contributions to the international PiGMaP Consortium established in 1991 to develop a comprehensive linkage map for the pig. The Pig Research and Development Corporation, later to become Australian Pork Limited, soon began to fund research in my laboratory, continuing up until 2005, with continuous funding of a postdoctoral position from 1992, initially for Paul Le Tissier and subsequently for Yizhou Chen. This funding went on to enable a multi-institutional program of co-operative research involving Universities of Sydney, Melbourne and New England as well as the industry partner QAF (Bunge) Meat Industries in search of quantitative trait loci (QTL) for important traits involved in growth and productivity, meat quality and immune function." @default.
- W2571403643 created "2017-01-13" @default.
- W2571403643 creator A5065551248 @default.
- W2571403643 date "2012-01-01" @default.
- W2571403643 modified "2023-09-27" @default.
- W2571403643 title "Looking back to move forward - a personal perspective on pig molecular genetics from RFLPs to nextgen sequencing" @default.
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