FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2571837343> ?p ?o ?g. }

• W2571837343 abstract "Abstract Abstract 3934 Background: Mantle cell lymphoma (MCL) is a pre–germinal center neoplasm characterized by cyclin D1 overexpression resulting from translocation of the cyclin D1 gene on 11q13 to the promoter of the immunoglobulin heavy chain locus on 14q32. Since MCL is incurable with standard lymphoma therapies, new treatment approaches are needed that target specific biologic pathways. The bioactive polyphenol curcumin (Curc), derived from the rhizome of Curcuma longa Linn, has been shown to have pleiotropic activities related to its complex chemistry and its influence on multiple signaling pathways including NF-kB, Akt, Nrf2 and pathways involved in metastasis and angiogenesis. Curc has been shown to cause growth arrest and apoptosis of BKS-2 immature B-cell lymphoma by downregulating growth and survival promoting genes (Clin Immunol 1999; 93:152). However, because of poor aqueous solubility Curc has had limited clinical utility, so investigators have explored nanoparticle drug delivery approaches (J Nanobiotech 2007, 5:3, MCT 2010; 9:2255). We reasoned that effective and targeted drug delivery by nanoparticles required appropriate receptors to facilitate binding. We therefore screened lymphoma cell lines for receptors that recognize apolipoprotein (apo) A-1. We hypothesized that a novel discoidal nanoparticle (ND) consisting of apoA-1, phospholipid and Curc (Curc ND) would bind to such receptors to facilitate drug delivery. Methods: We compared biologic activity of free Curc vs. Curc-ND in MCL cell lines expressing receptors for apoA-1. Cell lines were grown and maintained in culture, treated, and apoptosis and cell cycle progression was measured by flow cytometry. Relevant signaling intermediates and presence of apoA-1 receptors were measured by immunoblotting using specific antibodies. Results: Granta and Jeko cells (both MCL cell lines) expressed apoA-1 receptors including class B scavenger receptor (SR-B1) and the ATP-binding cassette transporter of the sub-family G1 (ABCG1). To compare the pro-apoptotic effect of free Curc and Curc-ND, Granta cells were incubated with free Curc, Curc-ND, empty ND, and medium alone (untreated). Compared to medium alone, empty ND had no effect while free Curc (20 μM) induced apoptosis. Curc-ND produced a dose-dependent increase in apoptosis, with ∼70% apoptosis at 20 μM. To investigate the mechanism of Curc-ND induced apoptosis, apoptosis-related proteins were studied in cultured Granta cells. A time-dependent decrease in caspase-9 levels was observed following incubation with Curc-ND or free Curc. The decrease in caspase-9 seen with Curc-ND, however, occurs much earlier (between 2–4 h of incubation) than for free-Curc. Caspase-3 was undetectable after 16 h with either treatment. Loss of this band implies activation of caspase-3, which was confirmed by PARP cleavage, wherein a decrease in the 116 kD band was accompanied by an increase in the 85 kD cleavage product. Unlike free Curc, Curc-ND induced PARP cleavage even at 16 h of incubation, suggesting sustained drug release. Curc-ND downregulated cyclin D1, decreased Akt phosphorylation and enhanced cleavage of caspases-9 and -3, and PARP. In addition, Curc-ND induced G1 cell cycle arrest to a greater extent than free Curc in Granta and Jeko cells (Granta: Control 34% G1, Curc 37% G1, Curc-ND 46% G1; Jeko: Control 39% G1, Curc 49% G1, Curc-ND 54% G1). Conclusion: We have shown that the MCL cell lines Granta and Jeko express apoA-1 receptors, making them likely targets for discoidal nanoscale delivery vehicles stabilized with Apo-A1. These nanodisks, when carrying the polyphenol Curc, can result in increased caspase -dependent apoptosis, cell cycle arrest, downregulation of cyclin-D1 and decreased p-Akt. These data suggest that the pleiotropic polyphenol Curc has cell killing/arrest activity in MCL and that Curc-ND may be a potential therapeutic with drug targeting ability. Disclosures: Forte: Lypro Biosciences: Employment." @default.
• W2571837343 created "2017-01-26" @default.
• W2571837343 creator A5010130956 @default.
• W2571837343 creator A5010703102 @default.
• W2571837343 creator A5037687817 @default.
• W2571837343 creator A5057502768 @default.
• W2571837343 creator A5070528822 @default.
• W2571837343 creator A5079084423 @default.
• W2571837343 date "2010-11-19" @default.
• W2571837343 modified "2023-09-28" @default.
• W2571837343 title "The Bioactive Polyphenol Curcumin (diferuloylmethane) In Human Apolipoprotein A-1 Nanodisks Enhances Apoptosis and G1 Cell Cycle Arrest In Mantle Cell Lymphoma Compared with Free Curcumin" @default.
• W2571837343 doi "https://doi.org/10.1182/blood.v116.21.3934.3934" @default.
• W2571837343 hasPublicationYear "2010" @default.
• W2571837343 type Work @default.
• W2571837343 sameAs 2571837343 @default.
• W2571837343 citedByCount "0" @default.
• W2571837343 crossrefType "journal-article" @default.
• W2571837343 hasAuthorship W2571837343A5010130956 @default.
• W2571837343 hasAuthorship W2571837343A5010703102 @default.
• W2571837343 hasAuthorship W2571837343A5037687817 @default.
• W2571837343 hasAuthorship W2571837343A5057502768 @default.
• W2571837343 hasAuthorship W2571837343A5070528822 @default.
• W2571837343 hasAuthorship W2571837343A5079084423 @default.
• W2571837343 hasConcept C185592680 @default.
• W2571837343 hasConcept C190283241 @default.
• W2571837343 hasConcept C199835354 @default.
• W2571837343 hasConcept C203014093 @default.
• W2571837343 hasConcept C2777525834 @default.
• W2571837343 hasConcept C2778250585 @default.
• W2571837343 hasConcept C2779338263 @default.
• W2571837343 hasConcept C29537977 @default.
• W2571837343 hasConcept C502942594 @default.
• W2571837343 hasConcept C55493867 @default.
• W2571837343 hasConcept C62112901 @default.
• W2571837343 hasConcept C86803240 @default.
• W2571837343 hasConcept C98274493 @default.
• W2571837343 hasConceptScore W2571837343C185592680 @default.
• W2571837343 hasConceptScore W2571837343C190283241 @default.
• W2571837343 hasConceptScore W2571837343C199835354 @default.
• W2571837343 hasConceptScore W2571837343C203014093 @default.
• W2571837343 hasConceptScore W2571837343C2777525834 @default.
• W2571837343 hasConceptScore W2571837343C2778250585 @default.
• W2571837343 hasConceptScore W2571837343C2779338263 @default.
• W2571837343 hasConceptScore W2571837343C29537977 @default.
• W2571837343 hasConceptScore W2571837343C502942594 @default.
• W2571837343 hasConceptScore W2571837343C55493867 @default.
• W2571837343 hasConceptScore W2571837343C62112901 @default.
• W2571837343 hasConceptScore W2571837343C86803240 @default.
• W2571837343 hasConceptScore W2571837343C98274493 @default.
• W2571837343 hasLocation W25718373431 @default.
• W2571837343 hasOpenAccess W2571837343 @default.
• W2571837343 hasPrimaryLocation W25718373431 @default.
• W2571837343 hasRelatedWork W2010858234 @default.
• W2571837343 hasRelatedWork W2023658004 @default.
• W2571837343 hasRelatedWork W2077597241 @default.
• W2571837343 hasRelatedWork W2111820414 @default.
• W2571837343 hasRelatedWork W2116784189 @default.
• W2571837343 hasRelatedWork W2123205017 @default.
• W2571837343 hasRelatedWork W2138464975 @default.
• W2571837343 hasRelatedWork W2146748065 @default.
• W2571837343 hasRelatedWork W2156398530 @default.
• W2571837343 hasRelatedWork W2157528577 @default.
• W2571837343 hasRelatedWork W2335526988 @default.
• W2571837343 hasRelatedWork W2546960435 @default.
• W2571837343 hasRelatedWork W2563815016 @default.
• W2571837343 hasRelatedWork W2564666883 @default.
• W2571837343 hasRelatedWork W2568790842 @default.
• W2571837343 hasRelatedWork W2766456701 @default.
• W2571837343 hasRelatedWork W2890893807 @default.
• W2571837343 hasRelatedWork W2894271670 @default.
• W2571837343 hasRelatedWork W2979418214 @default.
• W2571837343 hasRelatedWork W2985617261 @default.
• W2571837343 isParatext "false" @default.
• W2571837343 isRetracted "false" @default.
• W2571837343 magId "2571837343" @default.
• W2571837343 workType "article" @default.